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Gamma irradiation of dried plant material: implications for the identification of plant pathogens employing molecular techniques

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Abstract

Five pathogen-host leaf tissue combinations were used to determine whether dried plant disease material gamma (g) irradiated at a level of 25 kGy retains diagnostic value when subjected to selected polymerase chain reactionbased assays. Diagnostic amplification of nucleic acids from all anticipated positive samples was successful following g irradiation. Variability in results is discussed in relation to sample handling.

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Authors and Affiliations

  1. Northern Australia Quarantine Strategy (NAQS), Australian Quarantine and Inspection Service (AQIS), Cairns International Airport, Box 96, 4870, Qld, Australia

    L. M. Jones & R. I. Davis

  2. Centre for Tropical Agriculture, Agri-Science Queensland (Department of Employment, Economic Development and Innovation), PO Box 1054, 4880, Mareeba, Qld, Australia

    K. R. E. Grice

Authors
  1. L. M. Jones
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  2. K. R. E. Grice
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  3. R. I. Davis
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Correspondence to L. M. Jones.

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Jones, L.M., Grice, K.R.E. & Davis, R.I. Gamma irradiation of dried plant material: implications for the identification of plant pathogens employing molecular techniques. Australasian Plant Pathol. 39, 358–362 (2010). https://doi.org/10.1071/AP09093

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