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The development of mating type-specific primers for Mycosphaerella fijiensis, the causal agent of black Sigatoka of banana, and analysis of the frequency of idiomorph types in Mexican populations

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Abstract

A multiplex-PCR method was developed to identify the distribution and frequency of the mating types of the fungal banana leaf pathogen Mycosphaerella fijiensis in Mexico. PCR amplification identified different sized fragments for mat1-1 and mat1-2 genes. The same primers were also used to amplify fragments of the idiomorphs of Mycosphaerella musicola, another important banana pathogen closely-related to M. fijiensis. These were sequenced and were highly similar to M. fijiensis. Single conidial isolates of M. fijiensis were obtained from different fields in regions of Mexico not previously sampled, and from different banana and plantain cultivars, to determine the frequency and distribution of the mating types within natural populations. The multiplex primers and methods used for population analysis in M. fijiensis could also be applied to M. musicola populations andmay also be applicable to determine mating types in other closely related species, for a better understanding of the Mycosphaerella complex of pathogens and saprophytes associated with banana leaves. Additional keywords: epidemiology, fungal hybridisation, genetic mapping, virulence.

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Authors and Affiliations

  1. Centro de investigaciones Regionales ‘Dr Hideyo Noguchi’ Unidad Biomédicas, Universidad Autónoma de Yucatán, Av. Itzaes × 59., Mérida, C.P. 97000, Yucatán, México

    L. Conde-Ferráez

  2. Unidad de Biotecnología, Centro de Investigacion Cientifica de Yucatán, Calle 43 No. 130, Col. Churburná de Hidalgo, Mérida, C.P. 97200, Yucatán, México

    R. Grijalva-Arango, B. B. Canto-Canché & A. C. James

  3. Laboratorio de Biotecnología, Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Autopista Colima-Manzanillo km 40, Ap. Postal 36, C.P. 28100, Colima, México

    G. Manzo-Sánchez

  4. Division Academica, Centro de Investigacion Cientifica de Yucatán, Calle 43 No. 130, Col. Churburná de Hidalgo, Mérida, C.P. 97200, Yucatán, México

    M. I. Canul-Salazar

Authors
  1. L. Conde-Ferráez
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  2. R. Grijalva-Arango
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  3. B. B. Canto-Canché
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  4. G. Manzo-Sánchez
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  5. M. I. Canul-Salazar
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  6. A. C. James
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Correspondence to A. C. James.

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Conde-Ferráez, L., Grijalva-Arango, R., Canto-Canché, B.B. et al. The development of mating type-specific primers for Mycosphaerella fijiensis, the causal agent of black Sigatoka of banana, and analysis of the frequency of idiomorph types in Mexican populations. Australasian Plant Pathol. 39, 217–225 (2010). https://doi.org/10.1071/AP09086

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  • DOI: https://doi.org/10.1071/AP09086

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