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Polyphasic identification of Pseudomonas fuscovaginae causing sheath and glume lesions on rice in Australia

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Abstract

Pathogenic fluorescent pseudomonads associated with sheath brown rot disease symptoms on near-mature rice were characterised by a polyphasic study. Twelve strains of Pseudomonas fuscovaginae from the International Collection of Microorganisms from Plants (ICMP), which represent those strains lodged in several world culture collections, were used for comparison. Two strains, ICMP 9997 and 9999, were considered to have no similarity at all to P. fuscovaginae and a further two strains, ICMP11283 and 11284, were considered, by fatty acid analysis, to be more closely related to P. putida. These two strains were related to P. marginalis according to Biolog, and to P. tolaasii according to 16s and rpoB data. The Australian rice isolates were all identified as P. putida Biotype A by fatty acid analysis or as P. asplenii (4 isolates), P. fuscovaginae (2 isolates) or P. fluorescens (1 isolate) by Biolog. Sequencing of the 16s rRNA gene placed the rice isolates with P. fuscovaginae and P. asplenii, whereas rpoB gene sequence analysis showed a higher similarity to P. fuscovaginae than to P. asplenii. This is the first report of P. fuscovaginae in Australia.

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Author notes

  1. E. J. Cother

    Present address: New South Wales Department of Primary Industries, Agricultural Institute, Forest Road, PO Box 2117, 2800, Orange, NSW, Australia

Authors and Affiliations

  1. New South Wales Department of Primary Industries, Agricultural Institute, Forest Road, PO Box 2117, 2800, Orange, NSW, Australia

    D. H. Noble & R. J. van de Ven

  2. EHGraham Centre for Agricultural Innovation (Charles Sturt University & NSW Department of Primary Industries), PO Box 588, 2678, Wagga Wagga, NSW, Australia

    B. Stodart

  3. New South Wales Department of Primary Industries, Agricultural Institute, 2703, Yanco, NSW, Australia

    R. Reinke

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  1. E. J. Cother
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Correspondence to E. J. Cother.

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Cother, E.J., Stodart, B., Noble, D.H. et al. Polyphasic identification of Pseudomonas fuscovaginae causing sheath and glume lesions on rice in Australia. Australasian Plant Pathology 38, 247–261 (2009). https://doi.org/10.1071/AP08103

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