Australasian Plant Pathology

, Volume 37, Issue 1, pp 87–94 | Cite as

Effects of multiple applications of chemical elicitors on Botrytis cinerea infecting Geraldton waxflower

  • S.-Q. Dinh
  • D. C. Joyce
  • D. E. Irving
  • A. H. Wearing
Article

Abstract

Geraldton waxflower is susceptible to postharvest floral abscission caused by Botrytis cinerea infection. Pot- and field-grown waxflower cv. Mullering Brook plants and/or their harvested sprigs were spray treated with three known host plant defence elicitors, methyl jasmonate (MeJA), benzothiadiazole (BTH) or silicon (Si), and challenged with Botrytis cinerea after harvest. The efficacy of multiple MeJA, MeJA combined with BTH or Si and combined preand postharvest MeJA treatments in suppressing B. cinerea disease and floral abscission was assessed. Preharvest foliar applications of MeJA (1000 μM; two or four times), MeJA combined with BTH (150 mg/L) and MeJA combined with Si (1500 mg SiO2/L) generally did not suppress B. cinerea development and floral abscission from harvested sprigs. MeJA plus Si applied to pot-grown plants slightly and significantly (P<0.05) increased disease incidence and severity and flower fall. However, application of a MeJA postharvest (1000 μM) spray or pre- (1000 μM) plus postharvest (500 μM) MeJA sprays, significantly (P<0.05) reduced B. cinerea incidence and severity on sprigs from field-grown plants. Overall, the preharvest plus postharvest 1000 μMMeJA spray treatment consistently and significantly (P<0.05) suppressed B. cinerea infection on flowers from both pot- and field-grown plants. However, there was little or no impact of these treatments on floral abscission. Thus, multiple applications of chemical elicitors do not show promise as an approach to controlling B. cinerea-induced postharvest floral abscission in Geraldton waxflower.

Additional keywords

Chamelaucium uncinatum grey mould 

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References

  1. Beasley DR, Joyce DC (2002) A review of the flower characteristics of Geraldton waxflower and factors influencing their abscission from harvested stems. Australian Journal of Experimental Agriculture 42, 519–525. doi: 10.1071/EA01122CrossRefGoogle Scholar
  2. Beasley DR, Joyce D, Coates LM, Wearing AH (2001) Saprophytic microorganisms with potential for biological control of Botrytis cinerea on Geraldton waxflower flowers. Australian Journal of Experimental Agriculture 41, 697–703. doi: 10.1071/EA00112CrossRefGoogle Scholar
  3. Bokshi AI, Morris SC, McConchie RM, Deverall BJ (2006) Pre-harvest application of 2,6-dichloroisonicotinic acid, β-aminobutyric acid or benzothiadiazole to control post-harvest storage diseases of melons by inducing systemic acquired resistance (SAR). Journal of Horticultural Science & Biotechnology 81, 700–706.Google Scholar
  4. Bowen P, Menzies J, Ehret D, Samuels L, Glass ADM (1992) Soluble silicon sprays inhibit powdery mildew development on grape leaves. Journal of the American Society for Horticultural Science 117, 906–912.Google Scholar
  5. Chang P-FL, Xu Y, Narasimhan ML, Cheah KT, D’Urzo MP, Damsz B, Kononowicz AK, Abad L, Hasegawa PM, Bressan RA (1997) Induction of pathogen resistance and pathogenesis related genes in tobacco by a heat stable Trichoderma mycelial extract and plant signal messengers. Physiologia Plantarum 100, 341–352. doi: 10.1111/j.1399-3054.1997.tb04792.xCrossRefGoogle Scholar
  6. Conrath U, Beckers GJM, Flors V, Garcia-Agustin P, Jakab G, et al (2006) Priming: getting ready for battle. Molecular Plant-Microbe Interactions 19, 1062–1071. doi: 10.1094/MPMI-19-1062CrossRefPubMedGoogle Scholar
  7. Dann EK, Deverall BJ (2000) Activation of systemic disease resistance in pea by an avirulent bacterium or a benzothiadiazole, but not by a fungal leaf spot pathogen. Plant Pathology 49, 324–332. doi: 10.1046/j.1365-3059.2000.00457.xCrossRefGoogle Scholar
  8. Dann EK, Muir S (2002) Peas grown in media with elevated plant-available silicon levels have higher activities of chitinase and β-1,3-glucanase, are less susceptible to a fungal leaf spot pathogen and accumulate more foliar silicon. Australasian Plant Pathology 31, 9–13. doi: 10.1071/AP01047CrossRefGoogle Scholar
  9. Darras AI, Terry LA, Joyce DC (2005) Methyl jasmonate vapour treatment suppresses specking caused by Botrytis cinerea on cut Freesia hybrida L. flowers. Postharvest Biology and Technology 38, 175–182. doi: 10.1016/j.postharvbio.2005.06.011CrossRefGoogle Scholar
  10. Darras AI, Joyce DC, Terry LA (2006) Acibenzolar-S-methyl and methyl jasmonate treatments of glasshouse-grownfreesias suppress post-harvest petal specking caused by Botrytis cinerea. Journal of Horticultural Science & Biotechnology 81, 1043–1051.Google Scholar
  11. Desmond OJ, Edgar CI, Manners JM, Maclean DJ, Schenk PM, Kazan K (2006) Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiological and Molecular Plant Pathology 67, 171–179. doi: 10.1016/j.pmpp.2005.12.007CrossRefGoogle Scholar
  12. Díaz J, ten Have A, van Kan JAL (2002) The role of ethylene and wound signalling in resistance of tomato to Botrytis cinerea. Plant Physiology 129, 1341–1351. doi: 10.1104/pp.001453CrossRefPubMedGoogle Scholar
  13. Dinh S-Q, Joyce DC (2007) Prospects for cut-flower postharvest disease management with host defence elicitors. Stewart Postharvest Review 3, 1–11.CrossRefGoogle Scholar
  14. Dinh S-Q, Joyce DC, Irving DE, Wearing AH (2007) Field applications of three different classes of known host plant defence elicitors did not suppress Botrytis cinerea infecting Geraldton waxflower. Australasian Plant Pathology 36, 142–148. doi: 10.1071/AP07001CrossRefGoogle Scholar
  15. Epstein E (1999) Silicon. Annual Review of Plant Physiology and Plant Molecular Biology 50, 641–664. doi: 10.1146/annurev.arplant.50.1.641CrossRefPubMedGoogle Scholar
  16. Eyre JX, Joyce DC, Faragher J, Franz PR (2006) Effects of postharvest methyl jasmonate treatments against Botrytis cinerea on Geraldton waxflower (Chamelaucium uncinatum). Australian Journal of Experimental Agriculture 46, 717–723. doi: 10.1071/EA04250CrossRefGoogle Scholar
  17. Fauteux F, Remus-Borel W, Menzies JG, Bélanger RR (2005) Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiology Letters 249, 1–6.CrossRefPubMedGoogle Scholar
  18. Fawe A, Menzies JG, Chérif M, Bélanger RR (2001) Silicon and disease resistance in dicotyledons. In ‘Silicon in agriculture’. (Eds LE Datnoff, GH Snyder, GH Korndörfer) pp. 159–169. (Elsevier Science B.V: Amsterdam)CrossRefGoogle Scholar
  19. Friedrich L, Lawton K, Ruess W, Masner P, Specker N, et al (1996) A benzothiadiazole derivative induces systemic acquired resistance in tobacco. The Plant Journal 10, 61–70. doi: 10.1046/j.1365-313X. 1996.10010061.xCrossRefGoogle Scholar
  20. Hudgins JW, Franceschi VR (2004) Methyl jasmonate induced ethylene production is responsible for conifer phloem defense responses and reprogramming of stem cambial zone for traumatic resin duct formation. Plant Physiology 135, 2134–2149. doi: 10.1104/pp.103.037929CrossRefPubMedGoogle Scholar
  21. Jiang Y, Joyce DC, Terry LA (2001) 1-Methylcyclopropene treatment affects strawberry fruit decay. Postharvest Biology and Technology 23, 227–232. doi: 10.1016/S0925-5214(01)00123-5CrossRefGoogle Scholar
  22. Joyce DC (1988) Postharvest characteristics of Geraldton waxflowers. Journal of the American Society for Horticultural Science 13, 738–742.Google Scholar
  23. Joyce DC (1989) Treatments to prevent flower abscission in Geraldton wax. HortScience 24, 391.Google Scholar
  24. Joyce DC (1993) Postharvest floral organ fall in Geraldton waxflower (Chamelaucium uncinatum Schauer). Australian Journal of Experimental Agriculture 33, 481–487. doi: 10.1071/EA9930481CrossRefGoogle Scholar
  25. Little TM (1985) Analysis of percentage and rating scale data. HortScience 20, 642–644.Google Scholar
  26. Meir S, Droby S, Davidson H, Alsevia S, Cohen L, Horev B, Philosoph-Hadas S (1998) Suppression of Botrytis rot in cut rose flowers by postharvest application of methyl jasmonate. Postharvest Biology and Technology 13, 235–243. doi: 10.1016/S0925-5214(98)00017-9CrossRefGoogle Scholar
  27. Meir S, Droby S, Kochanek B, Salim S, Philosoph-Hadas S (2005) Use of methyl jasmonate for suppression of Botrytis rot in various cultivars of cut rose flowers. Acta Horticulturae 669, 91–98.Google Scholar
  28. Menzies J, Bowen P, Ehret D, Glass ADM (1992) Foliar application of potassium silicate reduce severity of powdery mildew on cucumber, muskmelon, and zucchini squash. Journal of the American Society for Horticultural Science 117, 902–905.Google Scholar
  29. Porat R, Borochov A, Halevy AH (1995) Is jasmonic acid involved in the endogenous regulation of Dendrobium orchid flower senescence? Acta Horticulturae 405, 314–319.Google Scholar
  30. Santamaria M, Thomson CJ, Read ND, Loake GJ (2001) The promoter of a basic PR1-like gene, AtPRB1, from Arabidopsis establishes an organ-specific expression pattern and responsiveness to ethylene and methyl jasmonate. Plant Molecular Biology 47, 641–652. doi: 10.1023/ A:1012410009930CrossRefPubMedGoogle Scholar
  31. Spoel SH, Koornneef A, Claessens SMC, Korzelius JP, Pelt JAV, et al (2003) NPR1 modulates cross-talk between salicylate- and jasmonatedependent defense pathways through a novel function in the cytosol. The Plant Cell 15, 760–770. doi: 10.1105/tpc.009159CrossRefPubMedGoogle Scholar
  32. Suo Y, Leung DWM (2002) BTH-induced accumulation of extracellular proteins and blackspot disease in rose. Biologia Plantarum 45, 273–279. doi: 10.1023/A:1015161110058CrossRefGoogle Scholar
  33. Taylor MN, Joyce DC, Wearing AH, Simons DH (1996) Control of postharvest pathogens of waxflower (Chamelaucium uncinatum). In ‘Fourth national workshop for Australian native flowers’. pp. 146–153. (The University of Western Australia: Perth)Google Scholar
  34. Terry LA, Joyce DC (2004) Elicitors of induced disease resistance in postharvest horticultural crops: a brief review. Postharvest Biology and Technology 32, 1–13. doi: 10.1016/j.postharvbio.2003.09.016CrossRefGoogle Scholar
  35. Thomma BPHJ, Eggermont K, Penninckx IAMA, Mauch-Mani B, Vogelsang R, Cammue BPA, Broekaert WF (1998) Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proceedings of the National Academy of Sciences of the United States of America 95, 15107–15111. doi: 10.1073/pnas.95.25.15107CrossRefPubMedGoogle Scholar
  36. Thomma BPHJ, Eggermont K, Broekaert WF, Cammue BPA (2000) Disease development of several fungi on Arabidopsis can be reduced by treatment with methyl jasmonate. Plant Physiology and Biochemistry 38, 421–427. doi: 10.1016/S0981-9428(00)00756-7CrossRefGoogle Scholar
  37. Tomas A, Wearing AH, Joyce DC (1995) Botrytis cinerea — a causal agent of premature flower drop in packaged Geraldton waxflower. Australasian Plant Pathology 24, 26–28. doi: 10.1071/APP9950026CrossRefGoogle Scholar
  38. Van Loon LC, Bakker PAHM, Pieterse CMJ (1998) Systemic resistance induced by rhizosphere bacteria. Annual Review of Phytopathology 36, 453–483. doi: 10.1146/annurev.phyto.36.1.453CrossRefPubMedGoogle Scholar
  39. Walters D, Walsh D, Newton A, Lyon G (2005) Induced resistance for plant disease control: maximizing the efficacy of resistance elicitors. Phytopathology 95, 1368–1373. doi: 10.1094/PHYTO-95-1368CrossRefPubMedGoogle Scholar
  40. Wilson CL, Solar JM, El Ghaouth A, Wisniewski ME (1997) Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Disease 81, 204–210. doi: 10.1094/ PDIS.1997.81.2.204CrossRefGoogle Scholar
  41. Xu Y, Chang P, Liu D, Narasimhan ML, Raghothama KG, Hasegawa PM, Bressan RA (1994) Plant defense genes are synergistically induced by ethylene and methyl jasmonate. The Plant Cell 6, 1077–1085. doi: 10.2307/3869886CrossRefPubMedGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2008

Authors and Affiliations

  • S.-Q. Dinh
    • 1
  • D. C. Joyce
    • 1
  • D. E. Irving
    • 1
  • A. H. Wearing
    • 1
  1. 1. Centre for Native Floriculture, School of Land, Crop and Food SciencesThe University of QueenslandGattonAustralia

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