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Characterisation of cineraria strain of Tomato yellow ring virus from Iran

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Abstract

A tospovirus isolated from naturally infected cineraria plants in commercial greenhouses in Shiraz, Iran, induced rings and chlorotic and necrotic spots on leaves, growth reduction and death of the plants. The viruswas transmitted by three populations of Thrips tabaci. Antisera were produced against purified virions and viral nucleocapsids and used to study the virus host range and relationships. On the basis of enzyme-linked immunosorbent assay, tomato, anemone, Arum sp., dandelion and pepper were natural hosts of the virus. SDS-PAGE revealed three major structural proteins corresponding to the Tospovirus N, G1 and G2 proteins. Three RNA segments were resolved in a 1% agarose gel with molecular masses of 1.02×106 Da, 1.87×106 Da and 2.86×106 Da, corresponding to the virus S, M and L RNAs, respectively. No serological relationships were detected between the cineraria virus and Tomato spotted wilt virus, Tomato chlorotic spot virus and Groundnut ring spot virus using the virus nucleocapsid IgG in dot immunobinding assays. Further characterisation by nucleotide sequencing of the virus N gene revealed that it was a strain of Tomato yellow ring virus, although the cineraria isolate appears to have a different experimental host range compared to tomato isolates of the virus.

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  1. K. Izadpanah

    Present address: Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran

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  1. Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran

    R. Rasoulpour

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  1. R. Rasoulpour
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  2. K. Izadpanah
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Correspondence to K. Izadpanah.

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Rasoulpour, R., Izadpanah, K. Characterisation of cineraria strain of Tomato yellow ring virus from Iran. Australasian Plant Pathology 36, 286–294 (2007). https://doi.org/10.1071/AP07023

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