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Australasian Plant Pathology

, Volume 31, Issue 3, pp 231–239 | Cite as

A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia

  • Lee A. McMichael
  • Denis M. PersleyEmail author
  • John E. Thomas
Article

Abstract

A previously undescribed tospovirus infecting tomato, capsicum and chilli in Queensland was characterised. The virus reacted with antibodies to serogroup IV tospoviruses, and the complete nucleotide sequence of its nucleocapsid protein gene indicated that it was a new tospovirus species. Sequence identities at the nucleotide and amino acid levels were <85% with recognised members of serogroup IV The virus infected test plants in five families and, importantly, overcame the Sw-5 resistance gene in tomato and TSWV resistance in Capsicum chinense accessions PI 152225, PI 159236 and AVRDC 00943. The name Capsicum chlorosis virus (CaCV) is proposed for this virus.

Additional keywords

surveys Tomato spotted wilt virus 

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References

  1. Bezerra IC, Resende R de O, Pozzer L, Nagata T, Kormelink R, De Avila AC (1999) Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology 89, 823–830.CrossRefPubMedGoogle Scholar
  2. Brittlebank CC (1919) Tomato diseases. Journal of the Department of Agriculture, Victoria 17, 231–235.Google Scholar
  3. Cho JJ, Custer DM, Brommonschenkel SH, Tanksley SD (1996) Conventional breeding: host-plant resistance and the use of molecular markers to develop resistance to tomato spot wilt virus in vegetables. Acta Horticulturae 431, 367–378.Google Scholar
  4. Cho JJ, Mau RFL, German TL, Hartmann RW, Yudin LS, Gonsalves D, Prowidenti R (1989) A multidisciplinary-approach to management of tomato spotted wilt virus in Hawaii. Plant Disease 73, 375–383.CrossRefGoogle Scholar
  5. De Avila AC, de Haan P, Kormelink R, Resende R de O, Goldbach RW, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequence. Journal of General Virology 74, 153–159.CrossRefPubMedGoogle Scholar
  6. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12, 387–395.CrossRefPubMedGoogle Scholar
  7. Gibbs A, McKenzie A (1997) A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods 63, 9–16.CrossRefPubMedGoogle Scholar
  8. Gibbs A, Mackenzie A, Blanchfield A, Cross P, Wilson C, Kitajima E, Nightingale M, Clements M (2000) Viruses of orchids in Australia: their identification, biology and control. The Australian Orchid Review June/July, 2000: 10–21.Google Scholar
  9. Goldbach R, Kuo G (1996) Introduction. Proceedings International symposium on tospoviruses and thrips of floral and vegetable crops. Taiwan Agricultural Research Institute, Taichung, Taiwan. Nov 7–10, 1995. Acta Horticulturae 431, 21–26.Google Scholar
  10. Hill MF, Moran JR (1996) The incidence of tomato spotted wilt tospovirus (TSWV) in Australian nursery plants. Australasian Plant Pathology 25, 114–119.CrossRefGoogle Scholar
  11. Jain RK, Pappu HR, Pappu SS, Krishna Reddy M, Vani A (1998) Watermelon bud necrosis tospovirus is a distinct virus species belonging to serogroup IV. Archives of Virology 143, 1637–1644.CrossRefPubMedGoogle Scholar
  12. Jones RAC, Cook D (2000) Proactive research and extension avoids damaging outbreak of WFT and TSWV in West Australian vegetable crops. WA Grower, June 2000: 2–3.Google Scholar
  13. Latham LJ, Jones RAC (1996) Tomato spotted wilt virus and its management. Journal of Agriculture-Western Australia 37, 86–91.Google Scholar
  14. Latham LJ, Jones RAC (1997) Occurrence of tomato spotted wilt tospovirus in native flora, weeds and horticultural crops. Australian Journal of Agricultural Research 48, 359–369.CrossRefGoogle Scholar
  15. Latham LJ, Jones RAC (1998) Selection of resistance breaking strains of tomato spotted wilt tospovirus. Annals of Applied Biology 133, 385–402.CrossRefGoogle Scholar
  16. McMichael LA, Persley DM, Thomas JE (2000) The first record of tospovirus serotype IV in Australia. Australasian Plant Pathology 29, 149.CrossRefGoogle Scholar
  17. Moury B, Palloix A, Gebre Selassie K, Marchoux G (1997) Hypersensitive resistance to tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains. Euphytica 94, 45–52.CrossRefGoogle Scholar
  18. Mumford RA, Barker I, Wood KR (1996) The biology of tospoviruses. Annals of Applied Biology 128, 159–183.CrossRefGoogle Scholar
  19. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence analysis. Proceedings of the National Academy of Sciences of the USA 85, 2444–2448.CrossRefPubMedGoogle Scholar
  20. Prins M, Goldbach R (1998) The emerging problem of tospovirus infection and non-conventional methods of control. Trends in Microbiology 6, 31–35.CrossRefPubMedGoogle Scholar
  21. Qiu W, Moyer JW (1999) Tomato spotted wilt tospovirus adapts to the TSWV N gene-derived resistance by genome reassortment. Phytopathology 89, 575–582.CrossRefPubMedGoogle Scholar
  22. Samuel G, Bald JG, Pittman HA (1930) Investigations on “spotted wilt” of tomatoes. Council for Scientific and Industrial Research Bulletin, Australia. Bulletin 44.Google Scholar
  23. Stevens MR, Scott SJ, Gergerich RC (1992) Inheritance of a gene for resistance to tomato spotted wilt virus from Lycopersicon peruvianum. Euphytica 59, 9–17.Google Scholar
  24. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–1680.CrossRefPubMedGoogle Scholar
  25. Wilson CR (1998) Incidence of weed reservoirs and vectors of tomato spotted wilt tospovirus on southern Tasmanian lettuce farms. Plant Pathology 47, 171–176.CrossRefGoogle Scholar
  26. Yeh SD, Chang TF (1995) Nucleotide sequence of the N gene of watermelon silver mottle virus, a proposed new member of the genus tospovirus. Phytopathology 85, 58–64.CrossRefGoogle Scholar
  27. Yeh SD, Sun IJ, Ho HM, Chang TF (1996) Molecular cloning and nucleotide sequence analysis of the S RNA of watermelon silver mottle tospovirus. Acta Horticulturae 431, 244–260.Google Scholar

Copyright information

© Australasian Plant Pathology Society 2002

Authors and Affiliations

  • Lee A. McMichael
    • 1
  • Denis M. Persley
    • 1
    Email author
  • John E. Thomas
    • 1
  1. 1.Department of Primary IndustriesAgency for Food and Fibre Sciences (Queensland Horticultural Institute)IndooroopillyAustralia

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