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Inoculation techniques for evaluating resistance to Curtobacterium flaccumfaciens pv. flaccumfaciens in mungbean cultivars

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Abstract

Inoculation methods were developed for the purpose of screening a large collection of mungbean germplasm lines for resistance to tan spot caused by Curtobacteriumflaccumfaciens pv. flaccumfaczens. Stem prick and leaf infiltration inoculations were used to induce symptoms of vascular wilt and leaf scorch, respectively. There was variation in reaction to the two different tests within a range of eight cultivars tested. Cv. Green Diamond showed tolerance in both tests. However, there was little correlation between the two tests in disease reaction. Evidence indicates that the leaf scorch symptom is also due to water deprivation rather than to apoplastic pathogen invasion. A stem prick test suitable for screening mungbeans for tolerance to tan spot is described.

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Authors and Affiliations

  1. Cunningham Laboratories, CSIRO Tropical Agriculture, 4067, St. Lucia, Queensland, Australia

    A. Diatloff & B. C. Imrie

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  1. A. Diatloff
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  2. B. C. Imrie
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Correspondence to A. Diatloff.

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Diatloff, A., Imrie, B.C. Inoculation techniques for evaluating resistance to Curtobacterium flaccumfaciens pv. flaccumfaciens in mungbean cultivars . Australasian Plant Pathology 29, 24–28 (2000). https://doi.org/10.1071/AP00004

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  • DOI: https://doi.org/10.1071/AP00004

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