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Australasian Plant Pathology

, Volume 39, Issue 2, pp 184–191 | Cite as

Genome sequences and phylogenetic placement of two isolates of Bean common mosaic virus from Macroptilium atropurpureum in north-west Australia

  • M. Saqib
  • S. Nouri
  • B. Cayford
  • R. A. C. Jones
  • M. G. K. Jones
Article

Abstract

In the Ord River irrigation area near Kununurra in the Kimberley region of Western Australia, symptoms suggestive of Bean common mosaic virus (BCMV) were found in a common leguminous weed, Macroptilium atropurpureum (siratro). Virus from one M. atropurpureum plant and an isolate from Phaseolus vulgaris (common bean) identified previously as BCMV based on genome fragment sequencing and host range, were both transmitted to Nicotiana benthamiana, Chenopodium amaranticolor and C. quinoa by sap inoculation, giving symptoms typical of BCMV.Reciprocal inoculations with infective N. benthamiana sap indicated that both source plants contained the same virus species and reproduced field symptoms consistent with BCMV in both original hosts. Complete (10 054 nucleotide) and partial (∼8400 nucleotide) sequences were determined for two sub-isolates MS1 and NWA-1, respectively, using reverse transcription—polymerase chain reaction assays. Both sub-isolates were from a N. benthamiana plant containing a BCMV isolate originally from a single M. atropurpureum plant. Their nucleotide sequences were aligned with those of four complete and three partial BCMVgenome sequences, and their coat protein amino acid sequences were aligned with those of 17 other BCMV isolates. The results from the genomic and coat protein analyses show that the two M. atropurpureum sub-isolates were closely related to BCMV isolates reported previously from North and South America.

Keywords

Australasian Plant Pathology Soybean Mosaic Virus Bean Common Mosaic Virus Coat Protein Gene Sequence Kimberley Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Akin HM, Sudarsono (1997) Characterisation of Peanut stripe virus (PStV) isolates originating from various provinces in Indonesia. Indonesian Journal of Tropical Agriculture 8, 13–20.Google Scholar
  2. Asensio-S-Manzanera MC, Asencio C, Singh SP (2006) Gamete selection for resistance to common and halo bacterial blight in dry bean intergene pool population. Crop Science 46, 131–135.CrossRefGoogle Scholar
  3. Berger PH, Wyatt SD, Shiel PJ, Silbernagel MJ, Druffel K, Mink GI (1997) Phylogenetic analysis of the Potyviridae with emphasis on legume-infecting potyviruses. Archives of Virology 142, 1979–1999. doi:10.1007/s007050050216CrossRefPubMedGoogle Scholar
  4. Bruun-Rasmussen M, Madsen CT, Johansen E, Albrechtsen M (2008) Revised sequence of Foxtail mosaic virus reveals a triple gene block structure similar to Potato virus X. Archives of Virology 153, 223–226. doi:10.1007/s00705-007-1057-3CrossRefPubMedGoogle Scholar
  5. Cassidy B, Sherwood JL, Nelson RS (1993) Cloning of the capsid protein gene from a blotch isolate of Peanut stripe virus. Archives of Virology 128, 287–297. doi:10.1007/BF01309440CrossRefPubMedGoogle Scholar
  6. Chen J, Chen J, Adams MJ (2001) A universal PCR primer to detect members of the Potyviridae and it use to examine the taxonomic status of several members of the family. Archives of Virology 146, 757–766. doi:10.1007/ s007050170144CrossRefPubMedGoogle Scholar
  7. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. The Journal of General Virology 34, 475–483. doi:10.1099/0022-1317-34-3-475CrossRefPubMedGoogle Scholar
  8. Collmer CW, Marston MF, Albert SM, Bajaj S, Maville HA, Ruuska SE, Vesely EJ, Kyle MM (1996) The nucleotide sequence of the coat protein gene and 3’ untranslated region of Azuki bean mosaic potyvirus, a member of the Bean common mosaic virus subgroup. Molecular Plant-Microbe Interactions 9, 758–761.CrossRefPubMedGoogle Scholar
  9. Davis RI, Thomas JE, McMichael LA, Dietzgen RG, Callaghan B, James AP, Gunua TG, Rahamma S (2002) Plant virus surveys on the island of New Guinea and adjacent regions of northern Australia. Australasian Plant Pathology 31, 385–390. doi:10.1071/AP02047CrossRefGoogle Scholar
  10. Drijfhout E, Morales FJ (2005) Bean common mosaic. In ‘Compendium of bean diseases’. (Eds HF Schwartz, JR Steadman, R Hall, RL Forster) pp. 62–63. (APS Press: St Paul, MN)Google Scholar
  11. Espert SM, Drewes SI, Burghardt AD (2007) Phylogeny of Macroptilium (Leguminosae): morphological, biochemical and molecular evidence. Cladistics 23, 119–129. doi:10.1111/j.1096-0031.2006.00140.xCrossRefGoogle Scholar
  12. Flasinski S, Gunasinghe UB, Gonzales RA, Cassidy BG (1996) The cDNA sequence and infectious transcripts of Peanut stripe virus. Gene 171, 299–300. doi:10.1016/0378-1119(96)00010-8CrossRefPubMedGoogle Scholar
  13. Gibbs AJ, Mackenzie AM, Wei K-J, Gibbs MJ (2008) The potyviruses of Australia. Archives of Virology 153, 1411–1420. doi:10.1007/s00705-008-0134-6CrossRefPubMedGoogle Scholar
  14. Ha CV, Coombs S, Revill PA, Harding RM, Vu MT, Dale JL (2008) Identification and sequence analysis of potyviruses infecting crops in Vietnam. Archives of Virology 153, 45–60. doi:10.1007/s00705-007-1067-1CrossRefPubMedGoogle Scholar
  15. Hall TA (1999) BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
  16. Harlan JR (1971) Agricultural origins: centers and noncenters. Science 174, 468–474. doi:10.1126/science.174.4008.468CrossRefPubMedGoogle Scholar
  17. Hu JS, Ferreria S, Wang M, Borth WB, Mink G, Jordan R (1995) Purification, host range, serology, and partial sequencing of dendrobium mosaic potyvirus, a new member of the Bean common mosaic virus subgroup. Phytopathology 85, 542–546. doi:10.1094/Phyto-85-542CrossRefGoogle Scholar
  18. Hutton EM (1962) Siratro, a tropical pasture legume bred from Phaseolus atropurpureus. Australian Journal of Experimental Agriculture and Animal Husbandry 2, 117–125. doi:10.1071/EA9620117CrossRefGoogle Scholar
  19. Khan JA, Lohuis D, Goldbach R, Dijkstra J (1993) Sequence data to settle the taxonomic position of Bean common mosaic virus and Blackeye cowpea mosaic virus isolates. The Journal of General Virology 74, 2243–2249. doi:10.1099/0022-1317-74-10-2243CrossRefPubMedGoogle Scholar
  20. Larsen RC, Micklas PN, Druffel KL, Wyatt SD (2005) NL-3Kstrain is a stable and naturally occurring interspecific recombinant derived from Bean common mosaic necrosis virus and Bean common mosaic virus. Phytopathology 95, 1037–1042. doi:10.1094/PHYTO-95-1037CrossRefPubMedGoogle Scholar
  21. Liu J, Peng X, Li L, Mang K (1993) Cloning of coat protein gene of Soybean mosaic virus and its expression in Escherichia coli. Chinese Journal of Biotechnology 9, 143–149.PubMedGoogle Scholar
  22. McKern NM, Shukla DD, Barnett OW, Vetten HJ, Dijkstra J, Whittaker LW, Ward CW (1992) Coat protein-properties suggest that Azuki bean mosaic virus, Blackeye cowpea mosaic-virus, Peanut stripe virus, and 3 isolates from soybean are all strains of the same potyvirus. Intervirology 33, 121–134.PubMedGoogle Scholar
  23. Melgarejo TA, Lehtonen MT, Fribourg CE, Rannali M, Valkonen JPT (2007) Strains of BCMV and BCMNV characterized from lima bean plants affected by deforming mosaic disease in Peru. Archives of Virology 152, 1941–1949. doi:10.1007/s00705-007-1008-zCrossRefPubMedGoogle Scholar
  24. Moghal SM, Francki RIB (1976) Towards a system for the identification and classification of potyviruses. I. Serology and amino acid composition of six distinct viruses. Virology 73, 350–362. doi:10.1016/0042-6822(76) 90396-2CrossRefPubMedGoogle Scholar
  25. Morales FJ, Bos L (1988) Bean common mosaic virus CMI/AAB descriptions. Plant Viruses 21, 6.Google Scholar
  26. Persley DM, McMichael L, Spence D (2001) Detection of Peanut stripe virus in post-entry quarantine in Queensland. Australasian Plant Pathology 30, 377. doi:10.1071/AP01051CrossRefGoogle Scholar
  27. Reddick D, Stewart VB (1919) Transmission of the virus of Bean common mosaic in seed and observations on the thermal death point of seed and virus. Phytopathology 9, 445–450.Google Scholar
  28. Regatieri LJ, Gaspar JO, Belintani P, Yuki VA (2008) Identification and characterization of a potyvirus isolated from siratro plants. Journal of Phytopathology 156, 214–216. doi:10.1111/j.1439-0434.2007.01342.xCrossRefGoogle Scholar
  29. Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F (1989) Nucleotide sequence of Potato virus Y (N strain) genomic RNA. The Journal of General Virology 70, 935–947. doi:10.1099/0022-1317-70-4-935CrossRefPubMedGoogle Scholar
  30. Saqib M, Jones RAC, Cayford B, Jones MGK (2005) First report of Bean common mosaic potyvirus in Western Australia. Plant Pathology 54, 563. doi:10.1111/j.1365-3059.2005.01232.xCrossRefGoogle Scholar
  31. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599. doi:10.1093/molbev/ msm092CrossRefPubMedGoogle Scholar
  32. Torrance L, Pead MT (1986) The application of monoclonal antibodies to routine tests for two plant viruses. In ‘Development and application in virus testing’. (Eds RAC Jones, L Torrance) pp. 103–118. (Lavenham Press Ltd: Suffolk)Google Scholar
  33. Yates RJ, Howieson JG, Nandasena KG, O’Hara GW (2004) Root-nodule bacteria from indigenous legumes in the north-west of Western Australia and their interaction with exotic legumes. Soil Biology & Biochemistry 36, 1319–1329. doi:10.1016/j.soilbio.2004.04.013CrossRefGoogle Scholar
  34. Zheng H, Chen J, Chen J, Adams MJ, Hou M (2002) Bean common mosaic virus isolates causing different symptoms in asparagus bean in China differ greatly in the 5’-parts of their genomes. Archives of Virology 147, 1257–1262. doi:10.1007/s00705-002-0805-7CrossRefPubMedGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2010

Authors and Affiliations

  • M. Saqib
    • 1
  • S. Nouri
    • 1
  • B. Cayford
    • 1
  • R. A. C. Jones
    • 2
  • M. G. K. Jones
    • 1
  1. 1.Plant Biotechnology Research Group, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and BiotechnologyMurdoch UniversityPerthAustralia
  2. 2.Agricultural Research Western AustraliaDepartment of Agriculture and FoodSouth PerthAustralia

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