Advertisement

Australasian Plant Pathology

, Volume 38, Issue 5, pp 461–466 | Cite as

Molecular differentiation of Mycosphaerella species from Musa

  • Y. Jaufeerally-Fakim
  • Dheema Sookun
  • S. Benimadhu
Article
  • 78 Downloads

Abstract

The three Mycosphaerella species M. fijiensis, M. musicola and M. eumusae, that infect bananas, cause major problems to the exploitation of this crop around the world. Their differentiation is crucial to the understanding of their epidemiology and control. M. eumusae was only recognised as a species in 2000, before which the other two were known to occur on Musa. It is now very commonly present in several countries. Bananas being a major source of revenue for low-income countries, they remain an important export commodity for these developing countries. The genetic relatedness of these three species was investigated through sequence analysis of conserved genes, actin, tubulin and histone 3. All confirmed M. eumusae as being different from the other two. Previous data relied on ribosomal sequences. Primers developed by Johanson and Jeger (1993) and which are specific for M. musicola and M. fijiensis, were tested on all three species. M. eumusae had an amplification profile which allowed its differentiation from the other two. The amplified fragments from the three species were sequenced and sequence polymorphism was used for designing new primers. Two new sets of primers, EuY1/EuY5 and EuY2/EuY5, reported here produced profiles that consistently gave the same bands which were specific for the three species and can be used in conventional PCR for their differentiation.

Keywords

Internal Transcribe Spacer Australasian Plant Pathology Actin Gene Conventional Polymerase Chain Reaction Single Spore 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arzanlou M, Abeln ECA, Kema GHJ, Waalwijk C, Carlier J, De Vries I, Guzman M, Crous PW (2007) Molecular diagnostics for the Sigatoka disease complex of banana. Phytopathology 97, 1112–1118. doi: 10.1094/PHYTO-97-9-1112CrossRefPubMedGoogle Scholar
  2. Ayliffe M, Dodds P, Lawrence G (2001) Characterisation of beta-tubulin from Melamspora lini and comparison of fungal beta-tubulin genes. Mycological Research 105, 818–826.CrossRefGoogle Scholar
  3. Blackwell M, Hibbett DS, Taylor JW, Spatafora JW (2006) Research coordination networks: a phylogeny for kingdom Fungi (Deep Hypha). Mycologia 98, 829–837. doi: 10.3852/mycologia.98.6.829CrossRefPubMedGoogle Scholar
  4. Carbone J, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91, 553–556. doi: 10.2307/3761358CrossRefGoogle Scholar
  5. Carlier J, Zapater MF, Lapeyre F, Jones DR, Mourichon X (2000) Septoria leaf spot of banana: a newly discovered disease caused by Mycosphaerella eumusae (Anamorph Septoria eumusae). Phytopathology 90, 884–890. doi: 10.1094/PHYTO.2000.90.8.884CrossRefPubMedGoogle Scholar
  6. Corradi N, Gerrit K, Sanders I (2004) Monophyly of beta-tubulin and H+ATPase gene variants in Glomus intraradices: consequences for molecular evolutionary studies of AM fungal genes. Fungal Genetics and Biology 41, 262–273. doi: 10.1016/j.fgb.2003.11.001CrossRefPubMedGoogle Scholar
  7. Crous PW, Groenewald JZ, Risede J-M, Hywel-Jones NL (2004) Calonectria species and their Cylindrocladium anamorphs: species with sphaeropedunculate vesicles. Studies in Mycology 50, 415–429.Google Scholar
  8. Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard J-F, Guindon S, Lefort V, Lescot M, Claverie J-M, Gascuel O (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research Jul 1; 36 (Web Server Issue), W465–469. Epub 2008 Apr 19. (PubMed)CrossRefPubMedGoogle Scholar
  9. Glass NL, Donaldson G (1995) Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61, 1323–1330.PubMedGoogle Scholar
  10. Johanson A, Jeger MJ (1993) Use of PCR for detection of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka leaf spots in banana and plantain. Mycological Research 97, 670–674. doi: 10.1016/ S0953-7562(09)80145-7CrossRefGoogle Scholar
  11. Johanson A, Templeton MD (1991) Preliminary studies of the use of PCR for random amplification of polymorphic DNA markers (RAPDs) in Mycosphaerella fijiensis and M. musicola. In ‘Proceedings of the tenth reunion meeting of ACORBAT, Villahermosa, Mexico, 2–9 November’.Google Scholar
  12. Johanson A, Crowhurst RN, Rikkerink EH, Fullerton RA, Templeton MD (1994) The use of species-specific DNA probes for the identification of Mycosphaerella fijiensis and M. musicola, the causal agent of Sigatoka disease of banana. Plant Pathology 43, 701–707. doi: 10.1111/j.1365-3059.1994.tb01609.xCrossRefGoogle Scholar
  13. Johanson A, Tushemereirwe WK, Karamura EB (2000) Distribution of Sigatoka leaf spots in Uganda as determined by species-specific polymerase chain reaction (PCR). Proceedings International Symposium On Banana and Plantains. (Eds K Craenen, R Ortiz, EB Karamura, DR Vuylsteke) Acta Horticultura. 540 ISHS, pp. 319–324.Google Scholar
  14. Keeling P (2003) Congruent evidence from a-tubulin and b-tubulin gene phylogenies for a zygomycete origin of microsporidia. Fungal Genetics and Biology 38, 298–309. doi: 10.1016/S1087-1845(02) 00537-6CrossRefPubMedGoogle Scholar
  15. Marín DH, Romero RA, Guzman M, Turner S (2003) Black Sigatoka: an increasing threat to banana cultivation. Plant Disease 87, 208–222. doi: 10.1094/PDIS.2003.87.3.208CrossRefGoogle Scholar
  16. O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS 2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7, 103–116. doi: 10.1006/mpev.1996.0376CrossRefPubMedGoogle Scholar
  17. Qianhong G, Han F, Dai J, Liu H, Guan H, Yu W (2005) Rapid isolation and sequence analysis of the beta-tubulin gene from Porphyra yezoensis (Rhodophyta) Journal of Applied Phycology 17, 1–5. doi: 10.1007/ s10811-005-5950-4CrossRefGoogle Scholar
  18. Surridge AKJ, Viljoen A, Crous PW, Wehner FC (2003) Identification of the pathogen associated with Sigatoka disease of banana in South Africa. Australasian Plant Pathology 32, 27–31. doi: 10.1071/ AP02058CrossRefGoogle Scholar
  19. Zapater MF, Rakotonantoandro A, Cohen S, Carlier J (2004) Polymerase chain reaction-restriction fragment length polymorphism markers for the fungal banana pathogen Mycosphaerella fijiensis. Molecular Ecology Notes 4, 80–82. doi: 10.1046/j.1471-8286.2003.00575.xCrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2009

Authors and Affiliations

  • Y. Jaufeerally-Fakim
    • 1
  • Dheema Sookun
    • 1
  • S. Benimadhu
    • 2
  1. 1.Faculty of AgricultureUniversity of MauritiusMauritius
  2. 2.Agricultural Research and Extension UnitMauritius

Personalised recommendations