Characterisation of Phytophthora infestans populations from Vietnam
- 98 Downloads
In 2002–03, 597 Phytophthora infestans isolates were collected from potato and tomato crops in nine provinces of Vietnam. A representative set of isolates were characterised for mating type, metalaxyl sensitivity, virulence, mitochondrial DNA (mt DNA) haplotype and RG57-fingerprint. All of the 294 tested isolates were of mating type A1. Metalaxyl-resistant isolates were found on both the potato and tomato hosts, and dominated the population in Lam Dong province (south of Vietnam). Nine known virulence genes were found among 27 tested isolates, although virulence to resistance gene R5 was not found. Among the 95 isolates tested for mtDNA haplotype, all were Ib type. Of these, all 34 isolates fingerprinted showed US-1-like genotype. Mating type, mtDNA haplotype and RG57-fingerprint data from this study indicate that the P. infestans population on tomato and potato from Vietnam still consist of the ‘old’ population.
Additional keywordDNA fingerprinting
Unable to display preview. Download preview PDF.
- Abad ZG, Abad JA (1997) Another look at the origin of late blight of potatoes, tomatoes, and pear melon in the Andes of South America. Plant Pathology 81, 682–688.Google Scholar
- Dao HC (2004) Potato in Vietnam. In ‘Proceedings of the regional workshop on potato late blight for East and Southeast Asia and the Pacific’. pp. 34–39. (Yezin Agricultural University: Myanmar) Available at: http://gilb.cip.cgiar.org/archive/article/archive/2005/november/article/regional-workshop-on-potato-late-blight-for-east-andsoutheast-asia-and-the-pacific/?cHash=bc60048ff4 [Verified 17 August 2008]Google Scholar
- Erselius LJ, Vega-Sánchez ME, Rodriquez AM, Bastidas O, Hohl HR, Ojiambo PS, Mukalazi J, Vermeulen T, Fry WE, Forbes GA (1998) Host specificity of Phytophthora infestans on tomato and potato in Uganda and Kenya. CIP Program Report 1997–1998, pp. 49–55.Google Scholar
- Gómez-Alpizar L, Carbone I, Ristaino JB (2007) An Andean origin of Phytophthora infestans inferred from mitochondrial and nuclear gene genealogies. Proceedings of the National Academy of Sciences of the United States of America 104, 3306–3311. doi: 10.1073/pnas. 0611479104CrossRefPubMedGoogle Scholar
- Jaime-Garcia R, Trinidad-Correa R, Felix-Gastelum R, Orum TV, Wasmann CC, Nelson MR (2000) Temporal and spatial patterns of genetic structure of Phytophthora infestans from potato and tomato in Del Fuerte Valley. Phytopathology 90, 1188–1195. doi: 10.1094/ PHYTO.2000.90.11.1188CrossRefPubMedGoogle Scholar
- Nguyen VV (1984) A new use for tissue culture and rapid multiplication: Potato production by Vietnamese farmers. Circular of the International Potato Center 12, 7–9.Google Scholar
- Nishimura R, Sato K, Lee WH, Singh UP, Chang T, et al. (1999) Distribution of Phytophthora infestans populations in seven Asian countries. Annals of the Phytopathological Society of Japan 65, 163–170.Google Scholar
- Pham XT (2000) Potato production in Vietnam. Dalat Research Center for FoodCrops, Dalat, Vietnam. Available at: http://www.eseap.cipotato.org/MF-ESEAP/Fl-Library/Pot-Vietnam.pdf [Verified 17 August 2008]Google Scholar
- Pham XT (2002) Breeding potatoes for late blight resistance in Vietnam. In ‘Proceedings of the global initiative on late blight conference.’ (Ed. C Lizárraga) p. 154. (International Potato Center: Lima, Peru)Google Scholar