Advertisement

Australasian Plant Pathology

, Volume 37, Issue 6, pp 592–599 | Cite as

Characterisation of Phytophthora infestans populations from Vietnam

  • V. H. LeEmail author
  • X. T. Ngo
  • M. B. Brurberg
  • A. Hermansen
Article

Abstract

In 2002–03, 597 Phytophthora infestans isolates were collected from potato and tomato crops in nine provinces of Vietnam. A representative set of isolates were characterised for mating type, metalaxyl sensitivity, virulence, mitochondrial DNA (mt DNA) haplotype and RG57-fingerprint. All of the 294 tested isolates were of mating type A1. Metalaxyl-resistant isolates were found on both the potato and tomato hosts, and dominated the population in Lam Dong province (south of Vietnam). Nine known virulence genes were found among 27 tested isolates, although virulence to resistance gene R5 was not found. Among the 95 isolates tested for mtDNA haplotype, all were Ib type. Of these, all 34 isolates fingerprinted showed US-1-like genotype. Mating type, mtDNA haplotype and RG57-fingerprint data from this study indicate that the P. infestans population on tomato and potato from Vietnam still consist of the ‘old’ population.

Additional keyword

DNA fingerprinting 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abad ZG, Abad JA (1997) Another look at the origin of late blight of potatoes, tomatoes, and pear melon in the Andes of South America. Plant Pathology 81, 682–688.Google Scholar
  2. Andrivon D, Béasse C, Laurent C (1994) Characterization of isolates of Phytophthora infestans collected in northwestern France from 1988 to 1992. Plant Pathology 43, 471–478. doi: 10.1111/j.1365-3059.1994. tb01580.xCrossRefGoogle Scholar
  3. Caten CE, Jinks JL (1968) Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Canadian Journal of Botany 46, 329–348.CrossRefGoogle Scholar
  4. Church GM, Gilbert W (1984) Genomic sequencing. Proceedings of the National Academy of Sciences of the United States of America 81, 1991–1995. doi: 10.1073/pnas.81.7.1991CrossRefPubMedGoogle Scholar
  5. Dao HC (2004) Potato in Vietnam. In ‘Proceedings of the regional workshop on potato late blight for East and Southeast Asia and the Pacific’. pp. 34–39. (Yezin Agricultural University: Myanmar) Available at: http://gilb.cip.cgiar.org/archive/article/archive/2005/november/article/regional-workshop-on-potato-late-blight-for-east-andsoutheast-asia-and-the-pacific/?cHash=bc60048ff4 [Verified 17 August 2008]Google Scholar
  6. Deahl KL, Cooke LR, Black LL, Wang TC, Perez FM, Moravec BC, Quinn M, Jones RW (2002) Population changes in Phytophthora infestans in Taiwan associated with the appearance of resistance to metalaxyl. Pest Management Science 58, 951–958. doi: 10.1002/ps.559CrossRefPubMedGoogle Scholar
  7. Drenth A, Tas ICQ, Govers F (1994) DNA fingerprinting un-covers a new sexually reproducing population of Phytophthora infestans in the Netherlands. European Journal of Plant Pathology 100, 97–107. doi: 10.1007/BF01876244CrossRefGoogle Scholar
  8. Erselius LJ, Vega-Sánchez ME, Rodriquez AM, Bastidas O, Hohl HR, Ojiambo PS, Mukalazi J, Vermeulen T, Fry WE, Forbes GA (1998) Host specificity of Phytophthora infestans on tomato and potato in Uganda and Kenya. CIP Program Report 1997–1998, pp. 49–55.Google Scholar
  9. Forbes GA, Goodwin SB, Drenth A, Oyarzun P, Ordoñez ME, Fry WE (1998) A global marker database for Phytophthora infestans. Plant Disease 82, 811–818. doi: 10.1094/PDIS.1998.82.7.811CrossRefGoogle Scholar
  10. Fry WE, Goodwin SB (1997) Re-emergence of potato and tomato late blight in the United States. Plant Disease 81, 1349–1357. doi: 10.1094/PDIS. 1997.81.12.1349CrossRefGoogle Scholar
  11. Fry WE, Goodwin SB, Dyer AT, Matuszak JM, Drenth A, Spielman LJ, Milgroom MG (1992) Population genetics and intercontinental migrations of Phytophthora infestans. Annual Review of Phytopathology 30, 107–129. doi: 10.1146/annurev.py.30.090192. 000543CrossRefGoogle Scholar
  12. Fry WE, Goodwin SB, Dyer AT, Matuszak JM, Drenth A, et al. (1993) Historical and recent migrations of Phytophthora infestans; chronology, pathways and implications. Plant Disease 77, 653–661.CrossRefGoogle Scholar
  13. Gisi U, Cohen Y (1996) Resistance to phenylamide fungicides: A case study with Phytophthora infestans involving mating type and race structure. Annual Review of Phytopathology 34, 549–572. doi: 10.1146/annurev. phyto.34.1.549CrossRefPubMedGoogle Scholar
  14. Gómez-Alpizar L, Carbone I, Ristaino JB (2007) An Andean origin of Phytophthora infestans inferred from mitochondrial and nuclear gene genealogies. Proceedings of the National Academy of Sciences of the United States of America 104, 3306–3311. doi: 10.1073/pnas. 0611479104CrossRefPubMedGoogle Scholar
  15. Goodwin SB (1997) The population genetics of Phytophthora. Phytopathology 87, 462–473.CrossRefPubMedGoogle Scholar
  16. Goodwin SB, Drenth A, Fry WE (1992 a) Cloning and genetic analysis of two highly polymorphic, moderately respective nuclear DNAs from Phytophthora infestans. Current Genetics 22, 107–115. doi: 10.1007/ BF00351469CrossRefPubMedGoogle Scholar
  17. Goodwin SB, Spielman LJ, Matuzak JM, Bergeron SN, Fry WE (1992b) Clonal diversity and genetic differentiation of Phytophthora infestans populations in northern and central Mexico. Phytopathology 82, 955–961. doi: 10.1094/Phyto-82-955CrossRefGoogle Scholar
  18. Goodwin SB, Cohen BA, Fry WE (1994) Panglobal distribution of a single clonal lineage of the Irish potato famine fungus. Proceedings of the National Academy of Sciences of the United States of America 91, 11591–11595. doi: 10.1073/pnas.91.24.11591CrossRefPubMedGoogle Scholar
  19. Goodwin SB, Sujkowski LS, Fry WE (1996) Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes of Phytophthora infestans in the United States and western Canada. Phytopathology 86, 793–800. doi: 10.1094/Phyto-86-793CrossRefGoogle Scholar
  20. Gotoh K, Askino S, Maeda A, Kondo N, Naito S, Kato M, Ogoshi A (2005) Characterization of some Asian isolates of Phytophthora infestans. Plant Pathology 54, 733–739. doi: 10.1111/j.1365-3059.2005.01286.xCrossRefGoogle Scholar
  21. Griffith GW, Shaw DS (1998) Polymorphismin Phytophthora infestans: Four mitochondrial haplotypes are detected following PCR amplification of DNA from pure cultures or from host lesions. Applied and Environmental Microbiology 64, 4007–4014.PubMedGoogle Scholar
  22. Hermansen A, Hannukkala A, Hafskjold Naerstad R, Brurberg MB (2000) Variation in populations of Phytophthora infestans in Finland and Norway: mating type, metalaxyl resistance and virulence phenotype. Plant Pathology 49, 11–22. doi: 10.1046/j.1365-3059.2000.00426.xCrossRefGoogle Scholar
  23. Hohl HR, Iselin K (1984) Strains of Phytophthora infestans from Switzerland with A2 mating type behaviour. Transactions of the British Mycological Society 83, 529–531.CrossRefGoogle Scholar
  24. Jaime-Garcia R, Trinidad-Correa R, Felix-Gastelum R, Orum TV, Wasmann CC, Nelson MR (2000) Temporal and spatial patterns of genetic structure of Phytophthora infestans from potato and tomato in Del Fuerte Valley. Phytopathology 90, 1188–1195. doi: 10.1094/ PHYTO.2000.90.11.1188CrossRefPubMedGoogle Scholar
  25. Koh YJ, Goodwin SB, Dyer AT, Cohen BA, Ogoshi A, Sato N, Fry WE (1994) Migrations and displacements of Phytophthora infestans populations in east Asian countries. Phytopathology 84, 922–927. doi: 10.1094/Phyto-84-922CrossRefGoogle Scholar
  26. McLeod A, Denman S, Sadie A, Denner FDN (2001) Characterization of South African isolates of Phytophthora infestans. Plant Disease 85, 287–291. doi: 10.1094/PDIS.2001.85.3.287CrossRefGoogle Scholar
  27. Nguyen VV (1984) A new use for tissue culture and rapid multiplication: Potato production by Vietnamese farmers. Circular of the International Potato Center 12, 7–9.Google Scholar
  28. Nishimura R, Sato K, Lee WH, Singh UP, Chang T, et al. (1999) Distribution of Phytophthora infestans populations in seven Asian countries. Annals of the Phytopathological Society of Japan 65, 163–170.Google Scholar
  29. Oyarzun PJ, Pozo A, Ordoñez ME, Doucett K, Forbes GA (1998) Host specificity of Phytophthora infestans on tomato and potato in Ecuador. Phytopathology 88, 265–271. doi: 10.1094/PHYTO.1998.88.3.265CrossRefPubMedGoogle Scholar
  30. Pham XT (2000) Potato production in Vietnam. Dalat Research Center for FoodCrops, Dalat, Vietnam. Available at: http://www.eseap.cipotato.org/MF-ESEAP/Fl-Library/Pot-Vietnam.pdf [Verified 17 August 2008]Google Scholar
  31. Pham XT (2002) Breeding potatoes for late blight resistance in Vietnam. In ‘Proceedings of the global initiative on late blight conference.’ (Ed. C Lizárraga) p. 154. (International Potato Center: Lima, Peru)Google Scholar
  32. Ristaino JB, Groves CT, Parra GR (2001)PCRamplification of the Irish potato famine pathogen from historic specimens. Nature 411, 695–697. doi: 10.1038/35079606CrossRefPubMedGoogle Scholar
  33. Shattock RC (1988) Studies on the inheritance of resistance to metalaxyl in Phytophthora infestans. Plant Pathology 37, 4–11. doi: 10.1111/j.1365-3059.1988.tb02188.xCrossRefGoogle Scholar
  34. Spielman LJ, Drenth A, Davidse LC, Sujkowski LJ, Gu WK, Tooley PW, Fry WE (1991) A second world-wide migration and population displacement of Phytophthora infestans. Plant Pathology 40, 422–430. doi: 10.1111/j.1365-3059.1991.tb02400.xCrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2008

Authors and Affiliations

  • V. H. Le
    • 1
    Email author
  • X. T. Ngo
    • 2
  • M. B. Brurberg
    • 1
  • A. Hermansen
    • 1
  1. 1.Bioforsk — The Norwegian Institute for Agricultural and Environmental ResearchPlant Health and Plant Protection DivisionÅsNorway
  2. 2.Hanoi Agricultural UniversityHanoiVietnam

Personalised recommendations