Australasian Plant Pathology

, Volume 36, Issue 2, pp 149–156 | Cite as

Genetic variation in Australian isolates of the grapevine pathogen Eutypa lata

Article

Abstract

Eutypa lata is an ascomycete fungus that causes eutypa dieback of grapevines, a disease which threatens vine productivity and longevity throughout the world. We assessed genetic variation among 35 isolates received as E. lata from Australia, Europe, California and South Africa using restriction fragment length polymorphism analysis. A subset of 10 isolates previously analysed for secondary metabolite production was also analysed using randomly amplified polymorphic DNA markers. Molecular analyses showed a high level of genetic diversity, with each isolate having a unique haplotype. Phylogenetic analysis did not allow separation of isolates based on geographic location or host species from which isolateswere obtained. Therewas no apparent correlation betweenDNAprofile and secondary metabolite production by E. lata. The results of this study showed a degree of genetic diversity similar to that reported for European and North American populations of E. lata, and are consistent with the hypothesis that the sole means of dispersal of the pathogen is by ascospores.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Carter M (1991) ‘The status of Eutypa lata as a pathogen.’ (CAB International: Wallingford, UK)Google Scholar
  2. Carter M, Bolay A, English H, Rumbos I (1985) Variation in the pathogenicity of Eutypa lata (= E. armeniacae). Australian Journal of Botany 33, 361–366. doi: 10.1071/BT9850361CrossRefGoogle Scholar
  3. Cortesi P, Milgroom MG (2001) Outcrossing and diversity of vegetative compatibility types in populations of Eutypa lata from grapevines. Journal of Plant Pathology 83, 79–86.Google Scholar
  4. Creaser M, Wicks T (2002) Short-term effects of remedial surgery to restore productivity to eutypa dieback-affected grapevines. Australian & New Zealand Grapegrower & Winemaker 461a, 73–75.Google Scholar
  5. DeScenzo R, Engel S, Gomez J, Munkvold G, Weller J, Irelan N (1999) Genetic analysis of Eutypa strains from California supports the presence of two pathogenic species. Phytopathology 89, 884–893.CrossRefPubMedGoogle Scholar
  6. Duthie J, Munkvold G, Marois J, Grant S, Chellemi D (1991) Relationship between age of vineyard and incidence of Eutypa dieback. Phytopathology 81, 1183.Google Scholar
  7. Egel D, Cotty P, Elias K (1994) Relationships among isolates of Aspergillus sect. flavi that vary in aflatoxin production. Phytopathology 84, 906–912.CrossRefGoogle Scholar
  8. Felsenstein J (1985) Confidence limits in phylogenies: an approach using the bootstrap. Evolution 39, 783–791. doi: 10.2307/2408678CrossRefGoogle Scholar
  9. Felsenstein J (1995) ‘Phylip (Phylogeny Inference Package) version 3.5c.’ (Department of Genetics, University ofWashington, Seattle, Distributed by the author)Google Scholar
  10. Hughes G, Munkvold GP, Samita S (1998) Application of the logistic-normal-binomial distribution to the analysis of Eutypadieback disease incidence. International Journal of Pest Management 44, 35–42. doi: 10.1080/096708798228509CrossRefGoogle Scholar
  11. Ju YM, Glawe DA, Rogers JD (1991) Conidial germination in Eutypa armeniacae and selected other species of Diatrypaceae: implications for the systematics and biology of diatrypaceous fungi. Mycotaxon 41, 311–320.Google Scholar
  12. Lardner R, Stummer B, Sosnowski M, Scott E (2005) Molecular identification and detection of Eutypa lata in grapevine. Mycological Research 109, 799–808. doi: 10.1017/S0953756205002893CrossRefPubMedGoogle Scholar
  13. Lardner R, Mahoney N, Zanker TP, Molyneux RJ, Scott ES (2006) Secondary metabolite production by the fungal pathogen Eutypa lata: analysis of extracts from grapevine cultures and detection of those metabolites in planta. Australian Journal of Grape and Wine Research 12, 107–114.CrossRefGoogle Scholar
  14. Link W, Dixens C, Singh M, Schwall M, Melchinger A (1995) Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD markers. Theoretical and Applied Genetics 90, 27–32. doi: 10.1007/BF00220992CrossRefGoogle Scholar
  15. Mahoney N, Lardner R, Molyneux R, Scott E, Smith L, Schoch T (2003) Phenolic metabolite profiles of the grapevine pathogen Eutypa lata. Phytochemistry 64, 475–484. doi: 10.1016/S0031-9422(03)00337-6CrossRefPubMedGoogle Scholar
  16. Mostert L, Crous P, Kang J-C, Phillips A (2001) Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia 93, 146–167.CrossRefGoogle Scholar
  17. Mulé G, Logrieco A, Stea G, Bottalico A (1997) Clustering of trichothecene-producing Fusarium strains determined from 28S ribosomal DNA sequences. Applied and Environmental Microbiology 63, 1843–1846.PubMedGoogle Scholar
  18. Munkvold G, Duthie J, Marois J (1994) Reductions in yield and vegetative growth of grapevines due to eutypa dieback. Phytopathology 84, 186–192.CrossRefGoogle Scholar
  19. Nei M, Li W (1979) A mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 76, 5269–5273. doi: 10.1073/pnas.76.10.5269CrossRefGoogle Scholar
  20. Péros J, Berger G (1999) Diversity within natural progenies of the grapevine dieback fungus Eutypa lata. Current Genetics 36, 301–309. doi: 10.1007/s002940050504CrossRefPubMedGoogle Scholar
  21. Péros JP, Berger G (2003) Genetic structure and variation in aggressiveness in European and Australian populations of the grapevine dieback fungus Eutypa lata. European Journal of Plant Pathology 109, 909–919. doi: 10.1023/B:EJPP.0000003648.10264.62CrossRefGoogle Scholar
  22. Péros J, Larignon P (1998) Confirmation of random mating and indication for gene flow in the grapevine dieback fungus, Eutypa lata. Vitis 37, 97–98.Google Scholar
  23. Péros JP, This P, Confuron Y, Chacon H (1996) Comparison by isozyme and RAPD analysis of some isolates of the grapevine dieback fungus, Eutypa lata. American Journal of Enology and Viticulture 47, 49–56.Google Scholar
  24. Péros J, Berger G, Lahogue F (1997) Variation in pathogenicity and genetic structure in the Eutypa lata population of a single vineyard. Phytopathology 87, 799–806.CrossRefPubMedGoogle Scholar
  25. Péros J, Jamaux-Despreaux I, Berger G, Gerba D (1999) The potential importance of diversity in Eutypa lata and co-colonising fungi in explaining variation in development of grapevine dieback. Mycological Research 103, 1385–1390. doi: 10.1017/S0953756299008291CrossRefGoogle Scholar
  26. Rogers S, Bendich A (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology 5, 69–76. doi: 10.1007/BF00020088CrossRefGoogle Scholar
  27. Sambrook J, Fritsch E, Maniatis T (1989) ‘Molecular cloning: a laboratory manual.’ (Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY)Google Scholar
  28. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503–517. doi: 10.1016/S0022-2836(75)80083-0CrossRefPubMedGoogle Scholar
  29. Stadler M, Wollweber H, Muhlbauer A, Asakawa Y, Hashimoto T, Rogers J, Ju Y, Wetzstein H, Tichy H (2001) Molecular chemotaxonomy of Daldinia and other Xylariaceae. Mycological Research 105, 1191–1205.CrossRefGoogle Scholar
  30. Tran-Dinh N, Pitt J, Carter D (1999) Molecular genotype analysis of natural toxigenic and nontoxigenic isolates of Aspergillus flavus and A. parasiticus. Mycological Research 103, 1485–1490. doi: 10.1017/S0953756299008710CrossRefGoogle Scholar
  31. Trouillas F, Gubler W (2004) Identification and characterization of Eutypa leptoplaca, a new pathogen of grapevine in Northern California. Mycological Research 108, 1195–1204. doi: 10.1017/ S0953756204000863CrossRefPubMedGoogle Scholar
  32. Van de Peer Y, De Wachter R (1994) TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Computer Applications in the Biosciences 10, 569–570.PubMedGoogle Scholar
  33. Vogel H (1964) Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98, 435–446. doi: 10.1086/282338CrossRefGoogle Scholar
  34. Wicks T, Hall B (1997) Eutypa dieback: a serious disease. Australian Grapegrower and Winemaker 405, 61–62.Google Scholar

Copyright information

© Australasian Plant Pathology Society 2007

Authors and Affiliations

  1. 1.Cooperative Research Centre for ViticultureGlen OsmondAustralia
  2. 2.School of Agriculture, Food and WineUniversity of AdelaideGlen OsmondAustralia

Personalised recommendations