Use of optical density as a measure of Claviceps africana conidial suspension concentration
- 60 Downloads
Sorghum ergot, caused by Claviceps africana, has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana, as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems.
Additional keywordscolorimeter inoculum concentration light transmittance Sorghum bicolor
Unable to display preview. Download preview PDF.
- Aberkane A, Cuenca-Estrella M, Gomez-Lopez A, Petrikkou E, Mellado E, Monzón A, Rodriguez-Tudela JL (2002) Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi. Journal of Antimicrobial Chemotherapy 50, 719–722. doi: 10.1093/jac/dkf187CrossRefPubMedGoogle Scholar
- Bhuiyan SA (2001) The biology and control of ergot (Claviceps africana) in sorghum. PhD Thesis, University of Queensland, Gatton Campus, Gatton, Australia.Google Scholar
- Blaney BJ, Kopinski J, Murray SA, McLennan S, Moss R, Downing J, Dingle J (2001) Research on the toxicity of sorghum ergot and its alkaloids. In ‘Proceedings of the 4th Australian Sorghum Conference’. (Eds AK Borrell, RG Henzell) (CD-ROM) (Range Media Pty Ltd.: Toowoomba, Qld)Google Scholar
- Genstat (2000) ‘Genstat for Windows. Release 4.2.’ 5th edn. (VSN International Ltd.: Oxford)Google Scholar
- McCullagh P, Neider JA (1989) ‘Generalized linear models.’ 2nd edn. (Chapman and Hall: London)Google Scholar
- Nagarajan K, Saraswathi V (1975) Production of “honeydew” like secretions in the culture of Sphacelia sorghi. Indian Phytopathology 28, 110.Google Scholar
- Ryley MJ, Persley DM, Jordan DR, Henzell RG (2002) Status of Sorghum and Pearl Millet Diseases in Australia. In ‘Sorghum and millets diseases’. (Ed. JF Leslie) pp. 441–448. (Iowa State University Press: Ames, IA)Google Scholar