Australasian Plant Pathology

, Volume 34, Issue 3, pp 287–292 | Cite as

Occurrence of bacterial soft rot of onion plants caused by Burkholderia gladioli pv. alliicola in Korea

  • C. J. Lee
  • J. T. Lee
  • J. H. Kwor
  • B. C. Kim
  • W. ParkEmail author


In the summer of 1999, disease symptoms of unknown origin were observed on onion bulbs in the fields and storage houses in Korea. Water soaked and very pale brown lesions appeared on the internal scales of diseased onion bulbs; they enlarged and extended to external scales with an associated sour smell. Bacterial soft rotting was more common than scale discoloration. This symptom was more pronounced at the neck end. From these lesions, we isolated two bacterial strains (designated CH1, CH2). Inoculation of bacterial isolates into onion bulbs yielded characteristic water-soaked symptoms which were distinguishable from those of the soft rot well known to onion growers. Inoculation of onion leaves and seed stalks produced white, oblong, slightly sunken and elongated spots. These symptoms started to appear 48 h after inoculation and leaves were completely killed within a week. Results of Gram stain, staining of flagella and biochemical tests identified these isolates as Burkholderia gladioli pv. alliicola. This was confirmed by pathogenicity, physiological and biochemical characteristics, an analysis of the 16S rRNA gene sequences and fatty acids profile. This is the first report of this bacterium in Korea.

Additional keywords

fatty acids first report 16S rRNA gene 


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  1. Abawi GS, Lorbeer JW (1971) Reaction of selected onion varieties to infection by Fusarium oxysporum f. sp. cepae. Plant Disease Reporter 55, 1000–1004.Google Scholar
  2. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389–3402. doi: 10.1093/nar/25.17.3389CrossRefPubMedGoogle Scholar
  3. Ballard RW, Palleroni NJ, Doudoroff M, Stanier RY (1970) Taxonomy of the aerobic Pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. Journal of General Microbiology 60, 199–214.PubMedGoogle Scholar
  4. Bazzi C (1979) Identification of Pseudomonas cepacia on onion bulbs in Italy. Phytopathology 95, 254–258.CrossRefGoogle Scholar
  5. Burkholder WH (1942) Three bacterial plant pathogens: Phytomonas caryophylli sp. n., Phytomonas alliicola sp. n., and Phytomonas manihotis (Arthaud-Berthet et Bondar) Viegas. Phytopathology 32, 141–149.Google Scholar
  6. Burkholder WH (1950) Sour skin, a bacterial rot of onion bulbs. Phytopathology 40, 115–117.Google Scholar
  7. Choi EH, Lee SE, Yoon KS, Kwon DK, Sohn JK, Park SH, Han MS, Ghim SY (2003) Isolation of nitrogen-fixing bacteria from gramineous crops and measurement of nitrogenase activity. Korean Journal of Microbiology and Biotechnology 31, 18–24.Google Scholar
  8. Cother EJ, Darbyshire B, Brewer J (1976) Pseudomonas aeruginosa: cause of internal brown rot of onion. Phytopathology 66, 828–834.CrossRefGoogle Scholar
  9. Dowson WJ (1957) ‘Plant disease due to bacteria.’ (Cambridge University Press: London)Google Scholar
  10. Head IM, Saunders JR, Pickup RW (1998) Microbial evolution, diversity, and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. Microbial Ecology 35, 1–21. doi: 10.1007/s002489900056CrossRefPubMedGoogle Scholar
  11. Henniger H (1965) Untersuchungen über knollen-, und lagerfäulen der kartoffel. 1. Zur methodik der resistenzprüfung mit erreger der bakteriellen knollennaßfäule (Pectobacterium carotovorum var. atrosepticum (van Hall) Dowson). Züchter 35, 174–180. doi: 10.1007/BF00707347Google Scholar
  12. Hevesi M, Viranyi F (1975) An unknown symptom on onion plants caused by Pseudomonas allicola. Acta Phytopathologica Academiae Scientiarum Hungaricae 10, 281–286.Google Scholar
  13. Irwin RD, Vaughan EK (1972) Bacterial rot of onion and the relation of irrigation water to disease incidence. Phytopathology 62, 1103 [Abstract].Google Scholar
  14. Joffe AZ, Ausher R, Palti J (1972) Distribution and pathogenicity of Fusarium species associated with onion in Israel. Phytopathology Mediterranae 11, 159–162.Google Scholar
  15. Jones LR (1901) A soft rot of carrot and other vegetables caused by Bacillus carotovorus. Vermont Agricultural Experiment Station Reporter 13, 299–332.Google Scholar
  16. Kasmire RF, Cantwell M (1992) Postharvest handling systems: Underground vegetables (roots, tubers and bulbs). In ‘Postharvest technology of horticultural crops’. (Ed. AA Kader) pp. 271–275. (The Regents of the University of California, Division of Agriculture and Natural Resources, University of California)Google Scholar
  17. Kawamoto SO, Lorbeer JW (1964) Selective isolation of soft rot bacteria of onion. Phytopathology 54, 897 [Abstract].Google Scholar
  18. Kawamoto SO, Lorbeer JW (1967) Soft rot bacteria associated with onion decay. Phytopathology 57, 341–342 [Abstract].Google Scholar
  19. Kawamoto SO, Lorbeer JW (1974) Infection of onion eaves by Pseudomonas cepacia. Phytopathology 64, 1440–1445.CrossRefGoogle Scholar
  20. Kishun R, Swarup J (1979) Further studies on bacterial stalk-rot of onion. Indian Phytopathology 32, 389–393.Google Scholar
  21. Klement Z, Farkas GL, Lovrekovich L (1964) Hypersensitive reaction induced by phytopathogenic bacteria in the tobacco leaf. Phytopathology 54, 474–477.Google Scholar
  22. MaCoy DC, Machtmes R (1974) Control of onion white-rot by furrow and root-dip application of fungicides. Plant Disease Reporter 58, 6–9.Google Scholar
  23. Maude RB, Presly AH (1977) Neck rot (Botrytis allii) of bulb onions. 1. Seed-borne infection in relationship to the disease in store and the effect of seed treatment. The Annals of Applied Biology 86, 181–188.CrossRefGoogle Scholar
  24. Moore ERB, Mau M, Arnscheidt A, Bottger EC, Hutson RA, Collins MD, Van de Peer Y, De Wächter R, Timmis KN (1996) The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas sensu stricto and estimation of the natural intrageneric relationships. Systematic and Applied Microbiology 19, 478–492.Google Scholar
  25. Ohuchi A, Ahaswa T, Nishimura J (1983) Two pathogenic bacteria, Erwinia rhapontici (Millard 1924) Burkholder 1948 and Pseudomonas marginalis pv. marginalis (Brown 1918) Stevens 1925, causing a soft rot of onion. Annals of the Phytopathological Society of Japan 49, 619–626.Google Scholar
  26. Palleroni NJ (1984) Genus Pseudomonas. In ‘Bergey’s manual of systematic bacteriology. Vol. 1’. (Eds NR Krieg, JG Hotr) pp. 141–219. (Williams and Wilkins: Baltimore)Google Scholar
  27. Palleroni NJ, Ballard RW, Ralston E, Doudoroff M (1972) Deoxyribonucleic acid homologies among some Pseudomonas species. Journal of Bacteriology 110, 1–11.PubMedGoogle Scholar
  28. Roberts P (1973) New or uncommon plant disease and pests. Plant Pathology 22, 98.CrossRefGoogle Scholar
  29. Sasser M J (1990) Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101, MIDI Inc., Newark, DE.Google Scholar
  30. Semeniuk G, Melthus IE (1943) Botany and plant pathology section. Republic Iowa Agricultural Experiment Station 1942–1943, 125–145.Google Scholar
  31. Stainer RY, Palleroni NJ, Doudoroff M (1966) The aerobic pseuomonads: a taxonomic study. Journal of General Microbiology 43, 159–271.Google Scholar
  32. Starr MP, Burkholder WH (1942) Lipolytic activity of phytopathogenic bacteria determined by means of spirit blue agar and its taxonomic significance. Phytopathology 32, 598–604.Google Scholar
  33. Swarup J, Nagarkoti MS, Saksena HK (1973) A new bacterial stalk-rot of onion caused by Pseudomonas alliicola in India. Indian Journal of Mycology Plant Pathology 3, 187–189.Google Scholar
  34. Tesoriero LA, Fahy PC, Gunn LV (1982) First record of a bacterial rot of onion in Australia caused by Pseudomonas gladioli pv. alliicola and association with internal browning caused by Pseudomonas aeruginosa. Australasian Plant Pathology 11, 56–57.CrossRefGoogle Scholar
  35. Ulrich JM (1975) Pectic enzymes of Pseudomonas cepacea and penetration of polygalacturonase into cells. Physiological Plant Pathology 5, 37–44.CrossRefGoogle Scholar
  36. Vitanov M (1970) Slippery skin of onion caused by Pseudomonas alliicola Burkholder. Gradinanska i Lozarska Nauka 7, 83–89.Google Scholar
  37. Vitanov M (1976) Influence of the harvest date and storage conditions on the slippery skin (Pseudomonas alliicola Burk.) infection of onions bulbs. Gradinanska i Lozarska Nauka 13, 63–71.Google Scholar
  38. Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M (1992) Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiology and Immunology 36, 1251–1275.PubMedGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2005

Authors and Affiliations

  • C. J. Lee
    • 1
  • J. T. Lee
    • 1
  • J. H. Kwor
    • 2
  • B. C. Kim
    • 3
  • W. Park
    • 3
    Email author
  1. 1.Onion Research InstituteGyeongnam Agricultural Research and Extension ServicesChangnyoungKorea
  2. 2.Gyeongnam Agricultural Research and Extension ServicesJinjuKorea
  3. 3.Department of MicrobiologyKyungpook National UniversityDeaguKorea

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