Australasian Plant Pathology

, Volume 34, Issue 2, pp 143–149 | Cite as

Cylindrocladium blight of . Eucalyptus grandis in Colombia

  • Carlos A. Rodas
  • Lorenzo Lombard
  • Marieka GryzenhoinfEmail author
  • Bernard Slippers
  • Michael J. Wingfield


Cylindrocladium leaf blight is one of the most important diseases affecting Eucalyptus grandis plantations in Colombia. Disease symptoms include both leaf blotch and shoot blight and these can lead to severe defoliation. This reduces the productivity of E. grandis in forestry zones with high humidity. The objective of this study was to identify the Cylindrocladium spp. associated with Cylindrocladium leaf blight of E. grandis in three important forestry regions of Colombia. Isolates were obtained from samples collected within these areas and morphology as well as DNA sequence data were used for identification. Results of both morphological comparisons and analysis of [ß-tubulin gene sequences showed that only C. spathulatum was associated with the disease symptoms in the evaluated areas. Evaluation of a Eucalyptus clonal trial showed that clones differ greatly in their susceptibility to infection by C. spathulatum. This presents excellent opportunities for disease avoidance in future.


Australasian Plant Pathology Eucalyptus Plantation Leaf Blight Scoparium Shoot Blight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Blum LEB, Dianese JC (1993) Susceptibility of different Eucalyptus genotypes to artificial leaf-inoculation with Cylindrocladium scopiarum and C. clavatum. European Journal of Forest Pathology 23, 276–280.CrossRefGoogle Scholar
  2. Booth H, Jovanovic T, Old KM, Dudzinski MJ (2000) Climatic mapping to identify high-risk areas for Cylindrocladium quinqueseptatum leaf blight on eucalypts in mainland South East Asia and around the world. Environmental Pollution (Barking, Essex: 1987) 108, 365–372. doi: 10.1016/S0269-7491 (99)00215-8CrossRefGoogle Scholar
  3. Crous PW (2002) ‘Taxonomy and pathology of Cylindrocladium (Calonectria) and allied genera.’ (The American Phytopathological Society: St Paul, MN, USA)Google Scholar
  4. Crous PW, Kang JC (2001) Phylogenetic confirmation of Calonectria spathulata and Cylindrocladium leucothoes based on morphology, and sequence data of the p-tubulin and ITS rRNA genes. Mycoscience 42, 51–57.CrossRefGoogle Scholar
  5. Crous PW, Wingfield MJ (1994) A monograph of Cylindrocladium, including anamorphs of Calonectria. Mycotaxon 51, 341–345.Google Scholar
  6. El-Gholl NE, Kimbrough JW, Barnard EL, Alfieri SA, Schoulties CL (1986) Calonectria spathulata sp. nov. Mycotaxon 16, 115–164.Google Scholar
  7. Glass NL, Donaldson G (1995) Development of primer sets for use with PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61, 1323–1330.PubMedGoogle Scholar
  8. Moller EM, Bahnweg G, Sandermann H, Geiger HH (1992) A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Research 20, 6115–6116.CrossRefPubMedGoogle Scholar
  9. O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS types with a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7, 103–116. doi: 10.1006/mpev. 1996.0376CrossRefPubMedGoogle Scholar
  10. Old KM, Wingfield MJ, Yuan ZQ (2003) ‘A manual of diseases of eucalypts in South-East Asia.’ (Centre for International Forestry Research: Jakarta, Indonesia)Google Scholar
  11. Osorio LF, Wright JW, White T (1995) Breeding strategy for Eucalyptus grandis at Smurfit Carton de Colombia. In ‘Eucalyptus plantations, improving fibre yield and quality’. (Eds BM Pitts, NMG Borralho, JB Reid, RN Cormer, WN Tibbits, CA Raymond) pp. 264–266. (CRC/IUFRO: Hobart, Australia)Google Scholar
  12. Park RF, Keane PJ, Wingfield MJ, Crous PW (2000) Fungal diseases of eucalypt foliage. In ‘Diseases and pathogens of eucalypts’. (Eds PJ Keane, GA Kile, FD Podger, BN Brown) pp. 153–239. (CSIRO Publishing: Collingwood, Australia)Google Scholar
  13. SAS Statistical Software (1990) ‘SAS / STAT User Guide’. Version 6, Fourth Edition. (SAS Institute: Cary, NC, USA)Google Scholar
  14. Schoch CL, Crous PW, Wingfield BD, Wingfield MJ (2001) Phylogenetics of Calonectria based on comparison of P-tubulin DNA sequences. Mycological Research 105, 1045–1052.CrossRefGoogle Scholar
  15. Sharma JK, Mohanan C (1991) ‘Epidemiology and control of diseases of Eucalyptus caused by Cylindrocladium spp. in India. Research report 70.’ (Kerala Forest Research Institute: Peechi, Kerala, India)Google Scholar
  16. Sharma JK, Mohanan C, Florence EJM (1985) ‘Disease survey in nurseries and plantations of forest tree species grown in Kerala. Research report 36.’ (Kerala Forest Research Institute: Peechi, Kerala, India)Google Scholar
  17. Swofford DL (2002) ‘PAUP. Phylogenetic analysis using parsimony.’ Version 4.0bl0. (Sinauer Associates Inc. Publishers: Sunderland, Massachussets, USA)Google Scholar
  18. Turnbull JW (2000) Economic and social importance of eucalypts. In ‘Diseases and pathogens of eucalypts’. (Eds PJ Keane, GA Kile, FD Podger, BN Brown) pp. 1–9. (CSIRO Publishing: Collingwood, Australia)Google Scholar
  19. Wingfield MJ (2003) 2003 Daniel McAlpine Memorial Lecture. Increasing threat of diseases to exotic plantation forests in the Southern Hemisphere: lessons from Cryphonectria canker. Australasian Plant Pathology 32, 133–139. doi: 10.1071/AP03024CrossRefGoogle Scholar
  20. Wingfield MJ, Roux J, Coutinho TA, Govender P, Wingfield BD (2001) Plantation disease and pest management in the next century. Southern African Forestry Journal 190, 67–71.Google Scholar

Copyright information

© Australasian Plant Pathology Society 2005

Authors and Affiliations

  • Carlos A. Rodas
    • 1
  • Lorenzo Lombard
    • 1
  • Marieka Gryzenhoinf
    • 1
    Email author
  • Bernard Slippers
    • 1
  • Michael J. Wingfield
    • 1
  1. 1.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa

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