Australasian Plant Pathology

, Volume 32, Issue 3, pp 411–420 | Cite as

Antifungal compounds in Geraldton waxflower tissues

Article

Abstract

Geraldton waxflower is the most economically importantnative Australian cut flower export. Infection of wax flower by Botrytis cinerea can lead to unacceptable levels of flower abscission after harvest. An investigation was conducted into the nature and identities of constitutive antifungal compounds in flowers and leaves. Antifungal activity against B, cinerea (pathogen) and Cladosporium cladosporioides (bioassay organism) was observed in leaf and flower extracts. Leaf tissue contained less antifungal activity than flower tissue. Four antifungal compounds were common to the three different waxflower cultivars studied. Two antifungal compounds were identified as the sesquiterpene, globulol, and the monoterpene, grandinol. At least two unidentified phenolic compounds also demonstrated strong antifungal activity. Notwithstanding general similarities in antifungal profiles, it was evident from TLC bioassays that variations exist in some antifungal compounds between different wax flower cultivars.

Addition al keywords

Botrytis cinerea thin layer chromatography bioassay 

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References

  1. Adikaram NKB, Joyce DC, Terry LA (2002) Biocontrol activity and induced resistance as a possible mode of action of Aureobasidium pullulans against grey mould of’strawberry fruit. Australasian Plant Pathology 31, 223–229.CrossRefGoogle Scholar
  2. Atkinson N (1949) Antibiotics in Australian native plants and fungi. The Medical Journal of Australia 1, 605–610.PubMedGoogle Scholar
  3. Atkinson N, Brice H (1955) Antibacterial substances produced by flowering plants. Australian Journal of Experimental Biology 33, 547–554.CrossRefGoogle Scholar
  4. Beasley DR (2001) ‘Strategies for control of Botrytis cinerea on Geraldton waxflower flowers’: PhD Thesis, The University of Queensland.Google Scholar
  5. Beasley DR, Joyce DC (2002) A review of the flower characteristics of Geraldton waxflower and factors affecting their abscission from harvested stems. Australian Journal of Experimental Agriculture 42, 519- 525.CrossRefGoogle Scholar
  6. Bhaskara Reddy MV, Angers P, Gosselin A, Arul J (1998) Characterization and use of essential oil from Thymus vulgaris against Botrytis cinerea and Rhizopus stolonifer in strawberry fruits. Phytochemistry 47, 1515–1520.CrossRefGoogle Scholar
  7. Brophy JJ, Goldsack RJ, Forster PI (2000) Essential oils of the genus Lophostemon (Myrtaceae). Flavour and Fragrance Journal 15, 17–20.CrossRefGoogle Scholar
  8. Chiba K, Arakawa T, Tada M (1998) Electrochemical synthesis of euglob-G1, -G2, -G3, -G4, -T1 and IIc. Journal of Chemical Society, Perkin Transactions 1, 2939–2942.CrossRefGoogle Scholar
  9. Droby S, Prusky D, Jacoby B, Golman A (1986) Presence of antifungal compounds in the peel of mango fruits and their relation to latent infections of Alternaria alternata. Physiological and Molecular Plant Pathology 29,173–183.CrossRefGoogle Scholar
  10. Egerton-Warburton LM, Ghisalberti EL (1995) Essential oil composition of Chamelaucium uncinatum. Phytochemistry 40, 837–839.CrossRefGoogle Scholar
  11. Egerton-Warburton LM, Ghisalberti EL, Considine JA (1998) Infraspecific variability in the volatile leaf oils of Chamelaucium uncinatum (Myrtaceae). Biochemical Systematics and Ecology 26, 873–888.CrossRefGoogle Scholar
  12. Joyce DC (1993) Postharvest floral organ fall in Geraldton waxflower (Chamelaucium uncinatum Schauer). Australian Journal of Experimental Agriculture 33, 481–487.CrossRefGoogle Scholar
  13. Joyce DC, Johnson GI (1999) Prospects for exploitation of natural disease resistance in harvested horticultural crops. Postharvest News and Information 10, 45N-48N.Google Scholar
  14. Joyce DC, Wearing AH (1996) Fungicides fight flower drop. Australian Horticulture 94, 58–59.Google Scholar
  15. Klarman WL, Stanford J B (1968) Isolation and purification of an antifungal principle from infected soybeans. Life Sciences 7, 1095–1103.CrossRefPubMedGoogle Scholar
  16. Lamers Y, Wijnberg H, de Groot A (2001) (+)- Aromadendrene as a chiral starting material in the synthesis of flavours fragrances and crop protecting agents. http://www.ftns.wau.nl/oc/research/ synthetic_organic_chem/Aromadendrene/aromadendrene.htm Wageningen University (6 November 2001).Google Scholar
  17. Olley CM, Joyce DC, Irving DE (1996) Changes in sugar, protein. respiration, and ethylene in developing and harvested Geraldton waxflower (Chamelaucium uncinatum) flowers. New Zealand Journal of Crop and Horticultural Science 24,143–150.CrossRefGoogle Scholar
  18. Rahalison L, Hamburger M, Hostettmann K, Monod M, Frenz E (1991) A bioautographic agar overlay method for detection of antifungal compounds from higher plants. Phytochemical Analysis 2, 199–203.CrossRefGoogle Scholar
  19. Taylor MN, Joyce DC, Wearing AH, Simons DH (1999) Evaluation of pyrimethanil (Scala) for the control of Botrytis cinerea on harvested Geraldton waxflower. Australian Journal of Experimental Agriculture 39, 639–641.CrossRefGoogle Scholar
  20. Taylor MN, Wearing AH, Joyce DC, Simons DH (1998) Alternaria alternata causes petal blight and flower drop in harvested Geraldton waxflower. Australasian Plant Pathology 27, 207- 210.CrossRefGoogle Scholar
  21. Terry LA, Joyce DC (2000) Suppression of grey mould on strawberry fruit with the chemical plant activator acibenzolar. Pest Management Science 56, 989–992.CrossRefGoogle Scholar
  22. Tomas A, Wearing AH, Joyce DC (1995) Botrytis cinerea, a causal agent of premature flower drop in packaged Geraldton waxflower. Australasian Plant Pathology 24, 26–28.CrossRefGoogle Scholar
  23. Waterman PG, Mole S (1994) ‘Analysis of phenolic plant metabolism.’ p. 146 (Blackwell Scientific Publications: Oxford)Google Scholar
  24. Wedge DE, Nagle DG (2000) A new 2D-TLC bioautography method for the discovery of novel antifungal agents to control plant pathogens. Journal of Natural Products 63, 1050–1054.CrossRefPubMedGoogle Scholar
  25. Wilson CL, Solar JM, El Ghaouth A, Wisniewski ME (1997) Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Disease 81, 204–210.CrossRefGoogle Scholar
  26. Zainuri, Joyce DC, Wearing AH, Coates L, Terry L (2001) Effects of phosphonate and salicylic acid treatments on anthracnose disease development and ripening of ‘Kensington Pride’ mango fruit. Australian Journal of Experimental Agriculture 41, 805–813.CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2003

Authors and Affiliations

  • L. A. Terry
    • 1
  • D. C. Joyce
    • 2
  • B. P. S. Khambay
    • 3
  1. 1.Plant Science Laboratory, Institute of BioScience and TechnologyCranfield University at SilsoeUK
  2. 2.Institute for Horticultural DevelopmentAgriculture VictoriaAustralia
  3. 3.Biological Chemistry DivisionRothamsted ResearchHarpendenUK

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