Australasian Plant Pathology

, Volume 32, Issue 3, pp 361–366 | Cite as

Ceratocystis fimbriata infecting Eucalyptus grandis in Uruguay

  • I. BarnesEmail author
  • J. Roux
  • B. D. Wingfield
  • M. O’Neill
  • M. J. Wingfield


Uruguay has a rapidly growing forestry industry consisting mainly of exotic Pinus and Eucalyptus spp. Recently, there have been reports of individual E. grandis trees wilting and dying rapidly in plantations. The aim of this investigation was to survey the dying E. grandis in the Rivera area of Uruguay and to determine the cause of the Eucalyptus wilt. Sap-staining symptoms were observed on recently pruned E. grandis. Discs of discoloured wood were cut from these pruned trees and from the stems of dying trees. These disks were stored in a moist environment to induce fungal sporulation. Ascomata, typical of a Ceratocystis sp., were found covering the edges of the wood where streaking symptoms occurred. Morphologically, the fungus resembles C. fimbriata. The internal transcribed spacer regions of the ribosomal RNA operon of the Ceratocystis sp. were amplified and sequenced. Sequence data confirmed placement of this fungus amongst other isolates of C. fimbriata. Furthermore, the sequence data showed that the Uruguay isolates are most closely related to those from diseased Eucalyptus spp. in Brazil, Congo and Uganda. C. fimbriata is a well-known pathogen of many woody plants and could constitute a serious threat to intensively managed E. grandis in Uruguay where the fungus was not previously known. The relationship between the pruning of E. grandis and infection by C. fimbriata will, in future, need to be evaluated.


Australasian Plant Pathology Internal Transcribe Space Region Australasian Plant Pathology Society Tree Bisection Reconnection Pruning Wound 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Australasian Plant Pathology Society 2003

Authors and Affiliations

  • I. Barnes
    • 1
    Email author
  • J. Roux
    • 1
  • B. D. Wingfield
    • 2
  • M. O’Neill
    • 3
  • M. J. Wingfield
    • 1
  1. 1.Deparrment of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.Department of GeneticsUniversity of PretoriaPretoriaSouth Africa
  3. 3.Uruguaya S. A. Forestry CompanyRiveraUruguay

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