Australasian Plant Pathology

, Volume 32, Issue 1, pp 1–7 | Cite as

The efficacy of phosphite a pp lied after inoculation on the colonisation of Banksia brownii sterns by Phytophthora cinnamomi

  • S. R. Barrett
  • B. L. Shearer
  • G. E. St J. Hardy


Low-volume phosphite application 2 days after stem inoculation significantly (P < 0.05) reduced colonisation of Banksia brownii by Phytophthora cinnamomi in a glasshouse trial at all phosphite application rates when compared with the control. There was a greater reduction in colonisation at rates of 24 and 96 kg/ha of phosphite, compared with the lowest rate of 12 kg/ha. The relationship between application rate and disease control was non-linear and suggested an optimum dose-response relationship. Foliar phytotoxicity at harvest, 9 days post-inoculation, was minimal at all application rates, although in planta phosphite concentrations were high. Growth of P. cinnamomi was not halted at any application rate at the time of harvest, but this may be due to the very high susceptibility of B. brownii to P. cinnamomi, the high virulence of the isolate used and the ideal temperature for growth of P. cinnamomi. The study suggests that low volume phosphite application to Phytophthora-infested plant communities may control the disease in individuals of plant species in the early stages of infection as well as protecting individuals that have avoided infection. Further studies on a range of native species are necessary to verify whether phosphite, applied post-infection, is effective in increasing plant survival.

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  1. Aberton MJ, Wilson BA, Cahill MJ (1999) The use of potassium phosphonate to control Phytophthora cinnamomi in native vegetation at Anglesea, Victoria. Australasian Plant Pathology 28, 225–234.CrossRefGoogle Scholar
  2. Afek U, Sztejnberg A (1989) Effects of fosetyl-Al and phosphorous acid on scoparone, a phytoalexin associated with resistance of citrus to Phytophthora citrophthora. Phytopathology 79, 736–739.CrossRefGoogle Scholar
  3. Allen DG, Jeffrey RC (1990) ‘Report of investigation no. 7. Methods for analysis of phosphorous in Western Australian soils.’ (Chemistry Centre of Western Australia: Perth)Google Scholar
  4. Ali Z, Guest DI (1998) Potassium phosphonate controls root rot of Xanthorrhoea australis and X. minor caused by Phytophthora cinnamomi. Australasian Plant Pathology 27, 40–44.CrossRefGoogle Scholar
  5. Anderson RD, Guest DI (1990) The control of black pod, canker and seedling blight of cocoa, caused by Phytophthora palmivora, with potassium phosphonate. Australasian Plant Pathology 19, 127–129.CrossRefGoogle Scholar
  6. Barchietto T, Saindrenan P, Bompeix G (1992) Physiological responses of Phytophthora citroph thora to a subinhibitory concentration of phosphonate. Pesticide Biochemistry and Physiology 42, 151–166.CrossRefGoogle Scholar
  7. Barrett S (2001) ‘Phytotoxic effects of phosphite in native plant communities in southern Western Australia.’ PhD Thesis, Murdoch University.Google Scholar
  8. Cohen Y, Coffey MD (1986) Systemic fungicides and the control of Oomycetes. Annual Review of Phytopathology 24, 311–338.CrossRefGoogle Scholar
  9. Dolan TE, Coffey MD (1988) Correlative in vitro and in vivo behaviour of mutant strains of Phytophthora palmivora expressing different resistance to phosphorous acid and fosetyl-Na. Phytopathology 78, 974–978.CrossRefGoogle Scholar
  10. Dunstan RH, Smillie RH, Grant BR (1990) The effects of sub-toxic levels of phosphonate on the metabolism and potential virulence factors of Phytophthora palmivora. Physiological and Molecular Plant Pathology 36, 205–220.CrossRefGoogle Scholar
  11. D’Arcy-Lameta A, Bompeix G, Reymond V, Soulie M-C (1989) Phosphonate uptake and distribution in healthy and infected leaves of tomato and cowpea. Plant Physiological Biochemistry 27, 919–924.Google Scholar
  12. De Boer RF, Greenhalgh FC (1990) Efficacy of potassium phosphonate in controlling Phytophthora root rot of subterranean clover and ornamental plants in Victoria. Australasian Plant Pathology 19, 124–125.CrossRefGoogle Scholar
  13. El-Hamalawi ZA, Menge JA (1995) Methods of fosetyl-Al application and phosphonate levels in avocado tissue needed to control stem canker caused by Phytophthora citricola. Plant Disease 79, 770–778.CrossRefGoogle Scholar
  14. Fenn ME, Coffey MD (1984) Studies on the in vitro and in vivo antifungal activity of fosetyl-Al and phosphorous acid. Phytopathology 74, 606–611.CrossRefGoogle Scholar
  15. Grant BR, Dunstan RH, Griffith JM, Niere JO, Smillie RH (1990) The mechanism of phosphonic (phosphorous) acid action in Phytophthora. Australasian Plant Pathology 19, 115–119.CrossRefGoogle Scholar
  16. Greenhalgh FC, de Boer RF, Merriman PR, Hepworth G, Keane PJ (1994) Control of Phytophthora root rot of irrigated subterranean clover with potassium phosphonate in Victoria, Australia. Plant Pathology 43, 1009–1019.CrossRefGoogle Scholar
  17. Griffith JM, Smillie RH, Niere JO, Grant BR (1989a) Effect of phosphate on the toxicity of phosphite in Phytophthora palmivora. Archives of Microbiology 152, 425–429.CrossRefGoogle Scholar
  18. Griffith JM, Akins LA, Grant BR (1989b) Properties of the phosphate and phosphite transport systems of Phytophthora palmivora. Archives of Microbiology 152, 430–436.CrossRefGoogle Scholar
  19. Groussal J, Delrot S, Caruhel P, Bonnemain J-L (1986) Design of an improved exudation method for phloem sap collection and its use for the study of phloem mobility of pesticides. Physiologie Vegetale 24, 123–133.Google Scholar
  20. Guest DI (1986) Evidence from light microscopy of living tissues that fosetyl-Al modifies the defence response in tobacco seedlings following inoculation by Phytophthora nicotianae var. nicotianae. Physiological and Molecular Plant Pathology 29, 251–261.CrossRefGoogle Scholar
  21. Guest D, Bompeix G (1990) The complex mode of action of phosphonates. Australasian Plant Pathology 19, 113–114.CrossRefGoogle Scholar
  22. Guest D, Grant BR (1991) The complex mode of action of phosphonates as antifungal agents. Biological Review 66, 159–187.CrossRefGoogle Scholar
  23. Holderness M (1990) Efficacy of neutralised phosphonic acid (phosphorous acid) against Phytophthora palmivora pod rot and canker of cocoa. Australasian Plant Pathology 19, 130–131.CrossRefGoogle Scholar
  24. Jackson T (1997) ‘Response of clonal Eucalyptus marginata plants to foliar applications of phosphite and the mode of phosphite action in controlling Phytophthora cinnamomi in P. cinnamomi resistant clonal jarrah.’ Report for the Neville Stanley Studentship in conjunction with Alcoa of Australia and Murdoch University.Google Scholar
  25. Jackson TJ, Burgess T, Colquhoun I, Hardy GE St J (2000) Action of the fungicide phosphite on Eucalyptus marginata inoculated with Phytophthora cinnamomi. Plant Pathology 49, 147–154.CrossRefGoogle Scholar
  26. Komorek B, Shearer BL, Smith B, Fairman RG (1994) ‘The control of Phytophthora in native plant communities.’ Annual Report to the Australian Nature Conservation Agency. (Department of Conservation and Land Management: Perth)Google Scholar
  27. Komorek B, Shearer BL, Smith B, Fairman RG (1997) The control of Phytophthora in native plant communities. In ‘Control of Phytophthora and Diplodina canker in Western Australia. Final Report to the Threatened Species and Communities, Biodiversity Group, Environment Australia’. (Ed. D Murray) pp. 1–59. (Department of Conservation and Land Management: Perth)Google Scholar
  28. Marks GC, Smith IW (1992) Metalaxyl and phosphonate as prophylactic and curative agents against stem infection of Leucodendron caused by Phytophthora cinnamomi. Australian Journal of Experimental Agriculture 32, 255–259.CrossRefGoogle Scholar
  29. McCredie TA, Dixon KW, Sivasithamparam K (1985) Variability in the resistance of Banksia L.f. species to Phytophthora cinnamomi Rands. Australian Journal of Botany 33, 629–637CrossRefGoogle Scholar
  30. Nemestothy GS, Guest DI (1990) Phytoalexin accumulation, phenylalanine ammonia lyase activity and ethylene biosynthesis in fosetyl-Al treated resistant and susceptible cultivars infected with Phytophthora nicotianae var nicotianae. Physiological and Molecular Plant Pathology 37, 207–219.CrossRefGoogle Scholar
  31. O’Gara E, Hardy GE St J, McComb J (1996) The ability of Phytophthora cinnamomi to infect through unwounded and wounded periderm tissue of Eucalyptus marginata. Plant Pathology 45, 955–963.CrossRefGoogle Scholar
  32. Ouimette DG, Coffey MD (1990) Symplastic entry and phloem translocation of phosphonate. Pesticide Biochemistry and Physiology 38, 18–25.CrossRefGoogle Scholar
  33. Pegg KG, Whiley AW, Saranah JB, Glass PJ (1985) Control of Phytophthora root rot of avocado with phosphorous acid. Australasian Plant Pathology 14, 25–29.CrossRefGoogle Scholar
  34. Pilbeam RA, Colquhoun IJ, Shearer B, Hardy GE St J (2000) Phosphite concentration: its effect on phytotoxicity symptoms and colonisation by Phytophthora cinnamomi in three understorey species of Eucalyptus marginata forest. Australasian Plant Pathology 29, 86–95.CrossRefGoogle Scholar
  35. Rohrback KG, Schenk S (1985) Control of pineapple heart rot, caused by Phytophthora parasitica and P. cinnamomi, with metalaxyl, fosetyl-Al, and phosphonic acid. Plant Disease 69, 320–323.Google Scholar
  36. Schutte GC, Bezuidenhout JJ, Kotze JM (1991) Timing of application of phosphonate fungicides using different application methods as determined by means of gas-liquid-chromatography for Phytophthora root rot control of citrus. Phytophylactica 23, 69–71.Google Scholar
  37. Seymour NP, Thompson JP, Fiske ML (1994) Phytotoxicity of fosetyl-Al and phosphonic acid to maize during production of vescicular-arbuscular mycorrhizal inoculum. Plant Disease 78, 441–446.CrossRefGoogle Scholar
  38. Shearer BL, Dillon M (1995) Susceptibility of plant species in Eucalyptus marginata forest to infection by Phytophthora cinnamomi. Australian Journal of Botany 43, 113–134.CrossRefGoogle Scholar
  39. Shearer BL, Fairman RG (1991) Control of Phytophthora species in native communities with phosphorous acid. In ‘Proceedings of the conservation biology in Australia and Oceania conference’, p. 72. (University of Queensland: Brisbane)Google Scholar
  40. Shearer BL, Fairman RG (1997) Foliar application of phosphite delays and reduces the rate of mortality of three Banksia species in communities infested with Phytophthora cinnamomi. In ‘Proceedings of the 11th biennial conference of the Australasian Plant Pathology Society’, p. 180. (Australasian Plant Pathology Society: Perth)Google Scholar
  41. Shearer BL, Shea SR, Degan PM (1987) Temperature-growth relationship of Phytophthora cinnamomi in the secondary phloem of roots of Banksia grandis and Eucalyptus marginata Phytopathology 77, 661–665.CrossRefGoogle Scholar
  42. Smillie R, Grant BR, Guest D (1989) The mode of action of phosphite, evidence for both direct and indirect modes of action on three Phytophthora spp. in plants. Phytopathology 79, 921–926.CrossRefGoogle Scholar
  43. Smith BJ (1994) ‘Effects of phosphonic acid and sodium silicate on lesion development of Phytophthora cinnamomi and histological responses in host species endemic to Western Australia.’ BSc (Hons) Thesis, University of Western Australia, Perth.Google Scholar
  44. Statsoft (1995) ‘Statistica for Windows.’ (Statsoft Inc.: Oklahoma)Google Scholar
  45. Tynan KM, Wilkinson CJ, Holmes JM, Dell B, Colquhoun IJ, McComb JA, Hardy GE St J (2001). The long-term ability of phosphite to control Phytophthora cinnamomi in two native plant communities of Western Australia. Australian Journal ofBotany 49, 761–770.CrossRefGoogle Scholar
  46. Walker GE (1989) Phytotoxicity in mandarins caused by phosphorous acid. Australasian Plant Pathology 18, 57–59.CrossRefGoogle Scholar
  47. Whiley AW, Hargreaves P, Pegg KG, Doogan V, Ruddle L, Saranah JB, Langdon PW (1995) Changing sink strengths influence translocation of phosphonate in avocado (Persea americana Mill.) trees. Australian Journal of Agricultural Research 46, 1079–1090.CrossRefGoogle Scholar
  48. Wicks JT, Hall B (1990) Evaluation of phosphonic (phosphorous) acid for the control of Phytophthora cambivora on almond and cherry in South Australia. Australasian Plant Pathology 19, 132–133.CrossRefGoogle Scholar
  49. Wilkinson C (1997) ‘The efficacy of phosphite in vitro and in planta in controlling the growth of Phytophthora cinnamomi and the production of sporangia and zoospores from lesions in Banksia grandis and Eucalyptus marginata.’ BSc (Hons) Thesis, Murdoch University.Google Scholar
  50. Wilkinson CJ, Holmes JM, Dell B, Tynan KM, McComb JA, Shearer BL, Colquhuon IJ, Hardy GE St J (2001) Ability of phosphite applied in a glasshouse trial to control Phytophthora cinnamomi in five plant species native to Western Australia. Australasian Plant Pathology 30, 343–351.CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society 2003

Authors and Affiliations

  • S. R. Barrett
    • 1
  • B. L. Shearer
    • 1
    • 2
  • G. E. St J. Hardy
    • 2
  1. 1.Department of Conservation and Land ManagementPerthAustralia
  2. 2.School of Biological Science and BiotechnologyMurdoch UniversityPerthAustralia

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