Abstract
Background, Aims, and Scope
There is an increasing demand for controlled toxicity tests to predict biological effects related to sediment metal contamination. In this context, questions of metal-specific factors, sensitivity of toxicity endpoints, and variability in exposure duration arise. In addition, the choice of the dose metrics for responses is equally important and is related to the applicability of the concept of critical body residue (CBR) in exposure assessments, as well as being the main focus of this study.
Methods
Experiments were conducted to assess toxicity of Cd, Cr, Cu and Pb to the oligochaete worm Lumbriculus variegatus with the aim of determining CBRs for two response metrics. Mortality and feeding activity of worms exposed to sediment-spiked metals were used as end-points in connection with residue analyses from both the organisms and the surrounding media.
Results
LC50 values were 0.3, 1.4, 5.2, and 6.7 mg/L (from 4.7 μmol/L to 128.0 μmol/L), and the order of toxicity, from most toxic to least toxic, was Cu > Cd > Pb>Cr. By relating toxicity to body residue, variability in toxicity among the metals decreased and the order of toxicity was altered. The highest lethal residue value was obtained for Cu (10.8 mmol/kg) and the lowest was obtained for Cd (2.3 mmol/kg). In the 10-d sublethal test, both time and metal exposure were an important source of variation in the feeding activity of worms. The significant treatment effects were observed from worms exposed to Cd or Pb, with the controls yielding the highest feeding rate. However, quantitative changes in the measured endpoint did not correlate with the exposure concentrations or body residues, which remained an order of magnitude lower than in the acute exposures.
Discussion
Both response metrics were able to detect a toxic effect of the metals. However, the ranking of metal toxicity was dependant on the choice of the dose metric used. An attempt to form a causal mortality-mediated link between tissue residues and metal toxicity was successful in water-only exposures. The results also indicated that egestion rate was a sensitive toxicity end point for predicting the effects of sediment contamination.
Conclusions
By relating the biological response with the tissue metal residues, toxicity data was comparable to both environmental media as well as different response metrics and time scales. The results also revealed the importance of metal toxicity ranking on a molar basis and, furthermore, a direct link to the CBR concept was established.
Recommendations and Perspectives
There is a growing demand for methods to assess the effects of contaminated sediments to benthic fauna and whole aquatic ecosystems. Such information is needed for sediment quality guidelines that are currently being developed in many countries and remediation processes. The use of body residues as a dose metric in metal toxicity studies may help to overcome difficulties related to bioavailability issues commonly faced in sediment toxicity studies.
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References
Allen Y, Calow P, Baird DJ (1995): A mechanistic model of contaminant-induced feeding inhibition in Daphnia magna. Environ Toxicol Chem 14, 1625–1630
Berry WJ, Boothman WS, Serbst JR, Edwards PA (2004): Predicting the toxicity of chromium in sediments. Environ Toxicol Chem 23, 2981–2992
Borgmann U (2000): Methods for assessing the toxicological significance of metals in aquatic ecosystems: Bioaccumulation-toxicity relationships, water concentrations, and sediment-spiking approaches. Aquat Ecosyst Health Manag 3, 277–289
Borgmann U, Couillard Y, Doyle P, Dixon DG (2005): Toxicity of sixty-three metals and metalloids to Hyalella azteca at two levels of water hardness. Environ Toxicol Chem 24, 641–652
Conder JM, Seals LD, Lanno RP (2002): Method for determining toxicologically relevant cadmium residues in the earthworm Eisenia fetida. Chemosphere 49, 1–7
Conder JM, Lanno RP (2003): Lethal critical body residues as measures of Cd, Pb, and Zn bioavailability and toxicity in the earthworm Eisenia fetida. J Soils Sediments 3, 13–20
DeCoen WM, Janssen CR (1998): The use of biomarkers in Daphnia magna toxicity testing I. The digestive physiology of daphnids exposed to toxic stress. Hydrobiology 367, 199–209
Crommentuijn T, Doodeman CJAM, Doornekamp A, Van Der Pol JJC, Bedaux JJM, Van Gestel CAM (1994): Lethal body concentrations and accumulation patterns determine time-dependent toxicity of cadmium in soil arthropods. Environ Toxicol Chem 13, 1781–1789
Gerhardt A (2007): Importance of exposure route for behavioural responses in Lumbriculus variegatus Müller (Oligochaeta: Lumbriculida) in short-term exposures to Pb. Env Sci Pollut Res 14(6) 430–434
Jager T, Heugens EHW, Kooijman SALM (2006): Making sense of ecotoxicological test results: Towards application of process-based models. Ecotoxicology 15, 305–314
Kooijman SALM, Bedaux JJM (1996): Analysis of toxicity tests on Daphnia survival and reproduction. Water Res 30, 1711–1723
Leppänen MT, Kukkonen JVK (1998a): Factors affecting feeding rate, reproduction and growth of an oligochaete Lumbriculus variegatus. Hydrobiologia 377, 183–194
Leppänen MT, Kukkonen JVK (1998b): Relationship between reproduction, sediment type, and feeding activity of Lumbriculus variegatus (Muller): Implications for sediment toxicity testing. Environ Toxicol Chem 17, 2196–2202
Lotufo GR, Fleeger JW (1996): Toxicity of sediment-associated pyrene and phenanthrene to Limnodrilus hoffmeisteri (Oligochaeta: Tubificidae). Environ Toxicol Chem 15, 1508–1516
Ma W (2005): Critical body residues (CBRs) for ecotoxicological soil quality assessment: Copper in earthworms. Soil Biol Biochem 37, 561–568
McCarty LS, Mackay D (1993): Enhancing ecotoxicological modeling and assessment. Environ Sci Technol 27, 1719–1727
Meador J (2006): Rationale and procedures for using the tissueresidue approach for toxicity assessment and determination of tissue, water, and sediment quality guidelines for aquatic organisms. Hum Ecol Risk Assess 12, 1018–1073
Milani D, Reynoldson TB, Borgmann U, Kolasa J (2003): The relative sensitivity of four benthic invertebrates to metals in spiked-sediment exposures and application to contaminated field sediment. Environ Toxicol Chem 22, 845–854
Moore DW, Dillon TM, Gamble EW (1995): Long-term storage of sediments: Implications for sediment toxicity testing. Environ Poll 89, 147–154
Norwood WP, Borgmann U, Dixon DG, Wallace A (2003): Effects of metal mixtures on aquatic biota: A review of observations and methods. Hum Ecol Risk Assess 9, 795–811
Nölte J (2003): ICP Emission Spectrometry, A Practical Guide, Wiley-VCH, Weinheim
Penttinen O-P, Kukkonen J, Pellinen J (1996): Preliminary study to compare body residues and sublethal energetic responses in benthic invertebrates exposed to sediment-bound 2,4,5-trichlorophenol. Environ Toxicol Chem 15, 160–166
Penttinen O-P, Kukkonen JVK (1998): Chemical stress and metabolic rate in aquatic invertebrates: Threshold, dose-response, and mode of toxic action. Environ Toxicol Chem 17, 883–890
Penttinen O-P, Kukkonen JVK (2006): Body-residues as dose for sublethal responses in alevins of landlocked salmon (Salmo salar m. sebago): A direct calorimetry study. Environ Toxicol Chem 25, 1088–1093
Penttinen S, Kukkonen J, Oikari A. (1995): The kinetics of cadmium in Daphnia magna as affected by humic substances and water hardness. Ecotoxicol Environ Saf 30, 72–76
Penttinen S, Kostamo A, Kukkonen JVK (1998): Combined effects of dissolved organic material and water hardness on toxicity of cadmium to Daphnia magna. Environ Toxicol Chem 17, 2498–2503
Poynton HC, Varshavsky JR, Chang B, Cavigiolio G, Chan S, Holman P, Loguinov AV, Bauer DJ, Komachi K, Theil EC, Perkins EJ, Hughes O, Vulpe CD (2007): Daphnia magna ecotoxicogenomics provides mechanistic insights into metal toxicity. Environ Sci Technol 41, 1044–1050
Schlemmer G, Radziuk B (1999): Analytical graphite furnace atomic absorption spectrometry, Birkhäuser Verlag, Berlin
Schlemmer G (1996): Graphite furnace AAS for complex samples: Detection limits, precision, long-term stability. At Spectrosc 17, 15–21
Schuler LJ, Landrum PF, Lydy MJ (2007): Response spectrum of fluoranthene and pentachlorobenzene for the fathead minnow (Pimephales promelas). Environ Toxicol Chem 26, 139–148
Shipp E, Grant A (2006): Hydrobia ulvae feeding rates: A novel way to assess sediment toxicity. Environ Toxicol Chem 25, 3246–3252
Simpson SL, Batley GE (2007): Predicting Metal Toxicity in Sediments: A Critique of Current Approaches. Integr Environ Assess Manag 3, 18–31
Schubauer-Berigan MK, Dierkes JR, Monson PD, Ankley GT (1993): pH-dependent toxicity of Cd, Cu, Ni, Pb and Zn to Ceriodaphnia pulex, Pimephales promelas, Hyalella azteca and Lumbriculus variegatus. Environ Toxicol Chem 12, 1261–1266
Vijver MG (2005): The ins and outs of bioaccumulation. Metal bioaccumulation kinetics in soil invertebrates in relation to availability and physiology. PhD thesis, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Vijver MG, Van Gestel CAM, Lanno RP, Van Straalen NM, Peijnenburg WJGM (2004): Internal metal sequestration and its ecotoxicological relevance — A review. Environ Sci Technol 38, 4705–4712
Vijver, MG, van Gestel, CAM, van Straalen, N, Lanno P, Peijnenburg WJGM (2006): Biological significance of metals partitioned to subcellular fractions within earthworms. Environ Toxicol Chem 25, 807–814
Williams PH (2005): Feeding behaviour of Lumbriculus variegates as an ecological indicator of in situ sediment contamination. PhD thesis, University of Stirling, Stirling, UK, 〈http://hdl.handle.net/1893/38〉
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Penttinen, OP., Kilpi-Koski, J., Jokela, M. et al. Importance of dose metrics for lethal and sublethal sediment metal toxicity in the oligochaete worm Lumbriculus variegatus . J Soils Sediments 8, 59–66 (2008). https://doi.org/10.1065/jss2007.12.267
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DOI: https://doi.org/10.1065/jss2007.12.267