Abstract
One of the main diagnostic problems in thyroid pathology is to distinguish between follicular adenoma and follicular carcinoma. Thorough sampling of the nodule’s capsule is recommended in order to identify capsular invasion. However, during the hardening of the tissue, by the usual fixatives the capsule shrinks and rolls downwards and sometimes the capsule separates from the remaining tissue. The present work evaluates the use of “Lymph Node Revealing Solution” (LNRS) for the rapid fixation of different thyroid lesions as compared to that of formalin. Fifty-one unselected consecutive cases of thyroid nodules, which included various benign and malignant lesions, were examined. Each specimen was cut in two equal parts; one was fixed in LNRS, the other in formalin. Fixation in LNRS for 2 hours gave adequate results in sectioning and staining of the tissue, and excellent immunostains. Its advantage over formalin is the conservation of the natural relationship between the capsule and the rest of the tissue, on the same plane, as well as the short time required for the final diagnosis.
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Koren R, Halpern M, Klein B, et al: A new rapid technique for the fixation of lymph nodes. Cell Vision 3:437–438, 1996.
Mc Marcus JFA, Mowry RW: Staining methods, histologic and histochemical. Harper & Brothers, New York, 1960, pp 19–21.
Lilie: Histologic technic and practical histochemistry. 3rd edition. Mc Grow-Hill Book Company, New York, 1965, pp 48–53.
Luna LG (ed): Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd edition. McGraw-Hill Book Company, New York, pp 1968, 4–5.
Hsu SM, Raine L, Fanger H: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580, 1981.
Bugis SP, Young JEM, Archibald SD, et al: Diagnostic accuracy of fine-needle aspiration biopsy versus frozen section in solitary nodules. Am J Surg 152:411–416, 1986.
Rosai J: Ackerman’s surgical pathology. Thyroid gland frozen section. 8th edition. Mosby, St. Louis, 1996, pp 545–546.
Prophet EB (ed): Laboratory methods in histotechnology: Armed Forces Institute of Pathology, Washington DC. 1992.
Noguchi M, Furuya S, Takeuchi T, et al: Modified formalin and methanol fixation methods for molecular biological and morphological analyses. Pathol Int 47:685–691, 1997.
Horbin RW: Problems and artifacts of microwave accelerated procedures in neurohistotechnology and resolution. Methods 15:101–106, 1998.
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Rath-Wolfson, L., Koren, R., Yaniv, E. et al. A New Rapid Technique for the Fixation of Thyroid Gland Surgical Specimens. Pathol. Oncol. Res. 5, 70–72 (1999). https://doi.org/10.1053/paor.1999.0070
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DOI: https://doi.org/10.1053/paor.1999.0070