Pathology & Oncology Research

, Volume 5, Issue 3, pp 214–217

Expression of membrane type 1-matrix metalloproteinase in laryngeal carcinoma

  • Bo Du 
  • Ping Wang 
  • Xiaofeng Guo 
  • Baodong Du 
Article

Abstract

Membrane type 1-matrix metalloproteinase (MT1-MMP) is a member of the recently identified unique membrane-type subgroup in the matrix metalloproteinase (MMP) family. MT1-MMP has proteolytic activity against components of the extracellular matrix and activates progelatinase A (MMP-2) at the cell surface. In this study, we analyzed the expression of MT1-MMP mRNA in 45 cases of laryngeal carcinoma by RT-PCR and investigated the relationship between MT1-MMP expression and survival in 18 cases. The result showed that the expression of MT1-MMP mRNA was higher in tumor tissue than in corresponding normal tissue. The tumoral expression in clinical stage III was higher than in stage II. The tumoral expression level of MT1-MMP mRNA in patients with lymph node metastasis was signigicantly higher than those with negative lymph nodes. The patients with high expression level showed significantly poorer 5 year survival than those with low expression level. Collectively, our findings suggest that the high level of MT1-MMP expression is closely related to the invasion and metastasis of laryngeal carcinoma, and indicates poorer prognosis.

Keywords

laryngeal carcinoma MT1-MMP invasion metastasis 

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References

  1. 1.
    Matrisian LM: Metalloproteinases and their inhibitors in matrix remodeling. Trends Genet 6:121–125, 1990.PubMedCrossRefGoogle Scholar
  2. 2.
    Woessner JFJ: Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J 5:2145–2154, 1991.PubMedGoogle Scholar
  3. 3.
    Cao J, Sato H, Takino T, et al: The C-terminal region of membrane type matrix metalloproteinase is a functional transmembrane domain required of pro-gelatinase A activation. J Biol Chem 270:801–805, 1995.PubMedCrossRefGoogle Scholar
  4. 4.
    Emonard HP, Remacle AG, Noel AC, et al: Tumor cell surface-associated binding site for the Mr 72.000 type IV collagenase. Cancer Res 52:5845–5848, 1992.PubMedGoogle Scholar
  5. 5.
    Pei D, Weiss S: Transmembrane-deletion mutants of the membrane-type matrix metalloproteinase-1 process proglatianse A and express intrinsic matrix-degrading activity. J Biol Chem 271:9135–9140, 1996.PubMedCrossRefGoogle Scholar
  6. 6.
    Kinoshita T, Sato H, Takino T, et al: Processing of a precursor of 72kDa type IV collagenase/gelatinase A by a recombinant membrane type 1 matrix metalloproteinase. Cancer Res 56:2535–2538, 1996.PubMedGoogle Scholar
  7. 7.
    Sato H, Takino T, Okada Y, et al: A matrix metalloproteinase expressed on the surface of invasive tumor cells. Nature 370:61–65, 1994.PubMedCrossRefGoogle Scholar
  8. 8.
    Bando E, Yonemura Y, Endou Y, et al: Immunohistochemical study of MTMMP tissue status in gastric carcinoma and correlation with survival analyzed by univariate and multivariate analysis. Oncol Rep 5: 1483–1488, 1998.PubMedGoogle Scholar
  9. 9.
    Mori M, Mimori K, Shiraishi T, et al: Analysis of MT1-MMP and MMP2 expression in human gastric cancers. Int J Cancer 74:316–321, 1997.PubMedCrossRefGoogle Scholar
  10. 10.
    YamamotoM, Mahanam S, Sawaya R, et al: Differential expression of membrane-type matrix metalloproteinase and its correlation with gelatinase A activation in human malignant brain tumors in vivo and in vitro. Cancer Res 56:384–392, 1996.PubMedGoogle Scholar
  11. 11.
    Gilles C, Polette M, Piette J, et al: High level of MT1-MMP expression is associated with invasiveness of cervical cancer cells. Int J Cancer 65:209–213, 1996.PubMedCrossRefGoogle Scholar
  12. 12.
    Tokuraku M, Sato H, Shinagawa A, et al: Activation of the precursor of gelatinase A/72 kDa type IV collagenase/MMP-2 in lung carcinoma correlation with the expression of membrane-type matrix metalloproteinase 1 (MT1-MMP) and with lymph node metastasis. Int J Cancer 64: 355–359, 1995.PubMedCrossRefGoogle Scholar
  13. 13.
    Yoshizaki T, Sato H, Maruyama Y, et al: Increased expression of membrane type 1-matrix metalloproteinase in head and neck carcinoma. Cancer 79:139–144, 1977.CrossRefGoogle Scholar
  14. 14.
    Répássy G, Forster-Horváth Cs, Juhász A, et al. Expression of invasion markers CD44v6/v3, nm23 and MMP2 in laryngeal and hypopharyngeal carcinoma. Pathol Oncol Res 4:14–22, 1998.PubMedCrossRefGoogle Scholar

Copyright information

© Arányi Lajos Foundation 1999

Authors and Affiliations

  • Bo Du 
    • 1
  • Ping Wang 
    • 1
  • Xiaofeng Guo 
    • 1
  • Baodong Du 
    • 1
  1. 1.Department of ENT & HN, First Clinical HospitalNorman Bethune University of Medical SciencesChang Chun, Ji LinPR China

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