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Inoculation of seven pine species or hybrid seedlings with Korean isolates of pinewood nematode under greenhouse conditions

Inoculation sous serre de semis de sept espèces ou hybrides de pin par des isolats coréens du nématode du pin

Abstract

  • • Seedlings of seven pine species or hybrids, Pinus densiflora, Pinus thunbergii, Pinus virginiana, Pinus echinata, Pinus koraiensis, Pinus thunbergii × Pinus massoniana and Pinus × rigitaeda, were inoculated in a greenhouse with 100, 1 000 and 10 000 pathogenic nematodes, Bursaphelenchus xylophilus, to compare initial symptom development and to evaluate susceptibility to pinewood nematodes.

  • • Genetic types were significantly different in the starting day of needle discoloration (χ2 = 43.48; P < 0.001) and the period from nematode inoculation to seedling death (χ2 = 32.85; P > 0.001), but treatment groups were not statistically different in the days from inoculation to seedling death in any of the pine species.

  • • There was no relationship between seedling mortality and the number of nematodes recovered from infected seedlings. Only eight of the nematode-inoculated seedlings remained alive by day 83 after inoculation, including four seedlings each of P. echinata and P. × rigitaeda grown from bulked seed collections.

  • • Seedlings from the bulk lots were likely more genetically diverse than those from the single-source lots. A broader genetic representation of pine species or hybrids is suggested for screening for planting stocks resistant to B. xylophilus.

Résumé

  • • Des semis de sept espèces ou hybrides de pin, Pinus densiflora, Pinus thunbergii, Pinus virginiana, Pinus echinata, Pinus koraiensis, Pinus thunbergii × massoniana, et Pinus × rigitaeda ont été inoculés sous serre par 100, 1000 ou 10 000 nématodes pathogènes Bursaphelenchus xylophilus, pour comparer le développement des symptômes à leur début et évaluer la sensibilité de ces essences.

  • • Des différences significatives ont été trouvées entre types génétiques pour la date de début de décoloration des aiguilles (χ2 = 43.48; P < 0.0001), ainsi que pour le délai entre l’inoculation et la mort des plants (χ2 = 32.85; P < 0.0001), mais pas entre traitements, quelle que soit l’espèce.

  • • Aucune relation n’a été observée entre la mortalité des semis et le nombre de nématodes récupérés des semis infestés. Seulement huit plants inoculés étaient encore vivants après 83 jours, dont quatre de P. echinata et de P. × rigitaeda issus des collections de graines en vrac.

  • • Les semis des lots de vrac étaient probablement génétiquement plus variés que ceux des lots de source unique. Une plus large représentation des espèces et des hybrides serait nécessaire pour un screening des souches de plantations résistantes à B. xylophilus.

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Correspondence to Do-Hyung Lee.

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Woo, KS., Lee, DH., Koo, YB. et al. Inoculation of seven pine species or hybrid seedlings with Korean isolates of pinewood nematode under greenhouse conditions. Ann. For. Sci. 65, 811 (2008). https://doi.org/10.1051/forest:2008072

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Keywords

  • pinewood nematode
  • susceptibility
  • inoculation
  • Bursaphelenchus xylophilus
  • mortality

Mots-clés

  • nématode du pin
  • sensibilité
  • inoculation
  • Bursaphelenchus xylophilus
  • mortalité