Skip to main content
SpringerLink
Log in

Genetic variation in natural populations of Abies nephrolepis Max. in South Korea

Variabilité génétique dans des populations naturelles de Abies nephrolepis Max. en Coréee du Sud

Annals of Forest Science volume 65, page 302 (2008)Cite this article

Abstract

Abies nephrolepis Max. is a fir species occurring in Northeast China, the extreme southeast of Russia and Korea. In Korea, A. nephrolepis is one of only three native fir species growing in high, mountainous areas with a disjunct distribution. We investigated genetic variation in A. nephrolepis by examining 24 ISSR polymorphic loci in 248 individuals representing eight natural populations in Korea. The level of intra-population genetic diversity (H e = 0.240) was similar to or slightly lower than that of plants with similar ecological traits and life history. Most of the genetic diversity was allocated among individuals within populations (G ST = 0.082, ΦST = 0.041) and the number of migrants per generation (N m ) was 5.56. Nei’s genetic distances were small (D = 0.027) and unrelated to geographic distances between populations. Implications for the conservation of genetic variation in A. nephrolepis in South Korea were discussed.

Résumé

Abies nephrolepis Max. est un sapin présent dans le Nord-Est de la Chine, l’extrême Sud-Est de la Russie et en Corée. En Corée, A. nephrolepis est une des trois espèces autochtones de sapin poussant dans des zones disjointes en haute montagne. Nous avons étudié la variabilité génétique chez A. nephrolepis grâce à 24 locus polymorphes ISSR sur un échantillon de 248 individus représentant huit populations naturelles en Corée. Le niveau de diversité génétique intra-population obtenu (H e = 0,240) était semblable ou légèrement plus faible que celui obtenu pour des plantes avec des caractères écologiques ou une histoire semblables. La plus grande part de la diversité génétique était représentée par la variabilité entre individus dans populations (G ST = 0,082, ΦST= 0,041) et le nombre de migrants par génération (N m) était de 5,56. Les distances génétiques de Nei étaient faibles (D = 0,027) et sans relation avec les distances géographiques entre populations. Les implications pour la conservation de la diversité génétique de A. nephrolepis en Corée du Sud sont discutées.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. Aagaard J.E., Krutovskii K.V., Strauss S.H., RAPDs and allozymes exhibit similar levels of diversity and differentiation among populations and races of Douglas-fir, Heredity 81 (1998) 69–78.

    Article  CAS  Google Scholar 

  2. Achere V., Favre J.M., Besnard G., Jeandorz S., Genomic organization of molecular differentiation in Norway spruce (Picea abies), Mol. Ecol. 14 (2005) 3191–3201.

    Article  PubMed  CAS  Google Scholar 

  3. Bohonak A.J., IBD (Isolation By Distance): a program for analyses of isolation by distance, J. Hered. 93 (2002) 153–154.

    Article  PubMed  CAS  Google Scholar 

  4. Chang C.S., Jeon J.I., Hyun J.O., An analysis of morphological variation in Abies koreana Wilson and A. nephrolepis (Traut.) Maxim, of Korea (Pinaceae) and their phylogenetic problems, J. Korean For. Soc. 86 (1997) 378–390 (in Korean).

    Google Scholar 

  5. Diaz V., Muniz L.M., Ferrer E., Random amplified polymorphic DNA and amplified fragment length polymorphism assessment of genetic variation in Nicaraguan populations of Pinus oocarpa, Mol. Ecol. 10 (2001) 2593–2603.

    Article  PubMed  CAS  Google Scholar 

  6. Excoffier L., Smouse P.E., Quattro J.M., Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data, Genetics 131 (1992) 179–191.

    Google Scholar 

  7. Excoffier L., Laval G., Schneider S., Arlequin ver. 3.0: An integrated software package for population genetics data analysis, Evol. Bioinform. Online 1 (2005) 47–50.

    CAS  Google Scholar 

  8. Farjon A., Rushforth K.D., A classification of Abies Mill. (Pinaceae), Notes of the Royal Botanic Garden Edinburgh 46 (1989) 59–79.

    Google Scholar 

  9. Farjon A., Pinaceae: Drawings and descriptions of the genera Abies, Cedrus, Pseudolarix, Keteleeria, Tsuga, Cathaya, Pseudotsuga, Larix and Picea, Königstein: Koeltz Scientific Books, 1990.

    Google Scholar 

  10. Godwin I.D., Aitken E.A.B., Smith L.W., Application of inter simple sequence repeat (ISSR) markers to plant genetics, Electrophoresis 18 (1997) 1524–1528.

    Article  PubMed  CAS  Google Scholar 

  11. Hamrick J.L., Godt M.J.W., Sherman-Broyles S.L., Factors influencing levels of genetic diversity in woody plant species, New For. 6 (1992) 95–124.

    Article  Google Scholar 

  12. Isabel N., Beaulieu J., Bousquet J., Complete congruence between gene diversity estimates derived from genotypic data at enzyme and random amplified polymorphic DNA loci in black spruce, Proc. Natl. Acad. Sci. USA 92 (1995) 6369–6373.

    Article  PubMed  CAS  Google Scholar 

  13. IUCN, 2006 IUCN Red List of Threatened Species. www.iucnredlist.org.

  14. Kim I.S., Hyun J.O., Genetic diversity of Abies koreana Wilson based on RAPD analysis, Korean J. Breed. 32 (2000) 12–18 (in Korean).

    Google Scholar 

  15. Kong W.S., Watts D., The plant geography of korea with an emphasis on the alpine zones, Kluwer Academic Publishers, Netherlands, 1993.

    Google Scholar 

  16. Kong W.S., Vegetation history of the Korean peninsula, Acanet, Seoul, Korea, 2003 (in Korean).

  17. Kwon H.Y., Kim Z.S., I-SSR variation within and among Korean populations in Taxus cuspidata, J. Korean For. Soc. 91 (2002) 654–660.

    Google Scholar 

  18. Ledig F.T., Hodgskiss P.D., Johnson D.R., Genetic diversity and seed production in Santa Lucia fir (Abies bracteata), a relict of the Miocene Broadleaved Evergreen Forest, Conserv. Genet. 7 (2006) 383–398.

    Article  CAS  Google Scholar 

  19. Lee S.W., Kim Y.Y., Hyun J.O., Kim Z.S., Comparison of genetic variation in Pinus densiflora natural populations by allozyme and RAPD analysis, Korean J. Breed. 29 (1997) 72–83 (in Korean).

    Google Scholar 

  20. Lee S.W., Ledig F.T., Johnson D.R., Genetic variation at allozyme and RAPD markers in Pinus longaeva (Pinaceae) of the White Mountains, California, Am. J. Bot. 89 (2002) 566–577.

    Article  PubMed  CAS  Google Scholar 

  21. Lee S.W., Kim Y.M., Kim W.W., Lack of allozyme and ISSR variation in the rare endemic tree species, Berchemia berchemiaefolia (Rhamnaceae) in Korea, Ann. For. Sci. 60 (2003) 357–360.

    Article  Google Scholar 

  22. Lee T.B., Dendrology, 4th ed., Hyang Moon Sa Publishing, Seoul, 1990 (in Korean).

    Google Scholar 

  23. Liu T.S., A monograph of the genus Abies, Taipei: The Department of Forestry College of Agriculture, National Taiwan University, 1971.

  24. Lynch M., Milligan B.G., Analysis of population genetic structure with RAPD markers, Mol. Ecol. 3 (1994) 91–99.

    Article  PubMed  CAS  Google Scholar 

  25. Mantel N., The detection of disease clustering and a generalized regression approach, Cancer Res. 27 (1967) 209–220.

    PubMed  CAS  Google Scholar 

  26. Mariette S., Chagne D., Lezier C., Pastuszka P., Raffln A., Plomion C., Kremer A., Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers, Heredity 86 (2001) 469–479.

    Article  PubMed  CAS  Google Scholar 

  27. Mosseler A., Egger K.N., Huches G.A., Low levels of genetic diversity in red pine confirmed by random amplified polymorphic DNA markers, Can. J. For. Res. 22 (1992) 1332–1337.

    Article  CAS  Google Scholar 

  28. Nei M., Analysis of gene diversity in subdivided populations, Proc. Natl. Acad. Sci. USA 70 (1973) 3321–3323.

    Article  PubMed  CAS  Google Scholar 

  29. Nei M., Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics 89 (1978) 583–590.

    PubMed  CAS  Google Scholar 

  30. Nybom H., Bartish I.V., Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants, Perspec. Plant Ecol. Evol. Syst. 3 (2000) 99–114.

    Google Scholar 

  31. Nybom H., Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants, Mol. Ecol. 13 (2004) 1143–1155.

    Article  PubMed  CAS  Google Scholar 

  32. Shannon C.E., A mathematical theory of communications, Bell System Tech. J. 27 (1948) 379–423.

    Google Scholar 

  33. Suyama Y., Yoshimaru H., Tsumura Y., Molecular phylogenetic position of Japanese Abies (Pinaceae) based on chloroplast DNA sequence, Mol. Phylogenet. Evol. 16 (2000) 271–277.

    Article  PubMed  CAS  Google Scholar 

  34. Szmidt A.E., Wang X.R., Lu M.Z., Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis, Heredity 76 (1996) 412–420.

    Article  CAS  Google Scholar 

  35. Tang S., Dai W., Li M., Zhang Y., Geng Y., Wang L., Zhong Y., Genetic diversity of relictual and endangered plant Abies ziyuanensis (Pinaceae) revealed by AFLP and SSR markers, Genetica (2007) DOI 10.1007/s10709-007-9178-x.

  36. Tsumura Y., Ohba K., Strauss S.H., Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica), Theor. Appl. Genet. 92 (1996) 40–45.

    Article  CAS  Google Scholar 

  37. Wolfe A.D., Liston A., Contributions of PCR-based methods to plant systematics and evolutionary biology, in: Soltis D.E., Soltis P.S., Doyle J.J. (Eds.), Plant molecular systematics II, Kluwer, 1998, pp. 43–86.

  38. Wu J., Krutovskii K.V., Strauss S.H., Nuclear DNA diversity, population differentiation, and phylogenetic relationships in the California closed-cone pines based on RAPD and allozyme markers, Genome 42 (1999) 893–908.

    Article  CAS  Google Scholar 

  39. Xiang Q.P., Xiang Q.Y., Liston A., Zhang X.C., Phylogenetic relationships in Abies (Pinaceae): evidence from PCR-RFLP of the nuclear ribosomal DNA internal transcribed spacer region, Bot. J. Linn. Soc. 145 (2004) 425–435.

    Article  Google Scholar 

  40. Xue X., Wang Y., Korpelainen H., Li C., Assessment of AFLP-based genetic variation in the populations of Picea asperata, Silvae Genet. 54 (2005) 24–30.

    Google Scholar 

  41. Yeh F.C., Yang R.C., Boyle T., POPGENE ver. 1.31 — Microsoft Window-based freeware for population genetic analysis, 1999.

Download references

Author information

Authors and Affiliations

  1. Department of Forest Genetic Resources, Korea Forest Research Institute, 441-350, Suwon, Rep. of Korea

    Lee Seok Woo, Yang Byeong Hoon, Han Sang Don, Song Jung Ho & Lee Jung Joo

Authors
  1. Lee Seok Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yang Byeong Hoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Han Sang Don
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Song Jung Ho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lee Jung Joo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lee Seok Woo.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Woo, L.S., Hoon, Y.B., Don, H.S. et al. Genetic variation in natural populations of Abies nephrolepis Max. in South Korea. Ann. For. Sci. 65, 302 (2008). https://doi.org/10.1051/forest:2008006

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1051/forest:2008006

search

Navigation