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Field effect of P fertilization on N2 fixation rate of Ulex europaeus

Effet in situ de la fertilisation en phosphore sur le taux de fixation de l’azote atmosphérique d’Ulex europaeus

Annals of Forest Science volume 64, pages 875–881 (2007)Cite this article

Abstract

European gorse (Ulex europaeus L.) N2 fixation rate (%Ndfa) was studied in a maritime pine (Pinus pinaster Aït.) oligotrophic forest. Fertilization field trials were carried out on 5 sites with various inputs of phosphorus (0–240 kg P2O5·ha−1). Seven to ten years after pine planting, gorse were sampled to evaluate the effect of P fertilization on gorse %Ndfa, determined using the 15N natural abundance method. One of the prerequisites of this method is the existence of a significant difference between the 15N/14N ratios in the atmospheric N reference and in the stand soil N references. This prerequisite was satisfied for 80 of 120 cases. The average %Ndfa was high (70 ± 3%) but with high local variability. No significant difference in %Ndfa was detected among P treatments. Nitrogen concentration of gorse was significantly higher in the highest dose treatments compared to the control.

Résumé

Le taux de fixation de l’azote atmosphérique (%Ndfa) de l’ajonc d’Europe (Ulex europaeus L.) a été étudié dans une forêt oligotrophe de pins maritimes. Des essais de fertilisation ont été établis avec plusieurs niveaux d’apport en phosphore (0–240 kg P2O5·ha−1). Sept à dix ans après la plantation de pins, les ajoncs ont été échantillonnés afin d’évaluer l’effet de la fertilisation en phosphore sur le %Ndfa, calculé par la méthode de l’abondance naturelle en 15N. Cette méthode nécessite notamment une différence significative entre les rapports 15N/14N de la référence atmosphérique et de la référence du sol des peuplements. Cette condition était satisfaite dans 80 cas sur 120. Le %Ndfa moyen était élevé (70 ± 3 %) mais avec une grande variabilité locale. Aucune différence des %Ndfa n’a été détectée entre les traitements. Les teneurs en azote des ajoncs étaient significativement plus élevées pour les doses maximales que pour les témoins.

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References

  1. Almeida J.P.F., Hartwig U.A., Frehner M., Nösberger J., Lüscher A., Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L.), J. Exp. Bot. 51 (2000) 1289–1297.

    Article  PubMed  CAS  Google Scholar 

  2. Amanuel G., Kühne R.F., Tanner D.G.,. Biological nitrogen fixation in faba bean (Vicia faba L.) in the Ethiopian highlands as affected by P fertilization and inoculation, Biol. Fertil. Soils 32 (2000) 353–359.

    Article  Google Scholar 

  3. Augusto L., Crampon N., Saur E., Bakker M.R., Jordan-Meille L., Pellerin S., de Lavaissière C., Trichet P., High rates of N2-fixation of Ulex species in the understory of Maritime pine stands (Pinus pinaster) and potential effect of P-fertilisation, Can. J. For. Res. 35 (2005) 1183–1192.

    Article  CAS  Google Scholar 

  4. Badarneh D.M.D., Magnitude of nitrogen fixation by lentil at different rates of phosphorus using 15N technique, J. Agron. Crop Sci. 175 (1995) 7–14.

    Article  Google Scholar 

  5. Boddey R.M., Peoples M.B., Palmer B., Dart P.J., Use of the 15N natural abundance technique to quantify biological nitrogen fixation by woody perennials, Nutr. Cycl. Agroecosys. 57 (2000) 235–270.

    Article  Google Scholar 

  6. Campillo R., Urquiaga S., Undurraga P., Pino I., Boddey R.M., Strategies to optimise biological nitrogen fixation in legume/grass pastures in the southern region of Chile, Plant Soil 273 (2005) 57–67.

    Article  CAS  Google Scholar 

  7. Cheaïb A., Mollier A., Thunot S., Lambrot C., Pellerin S., Loustau D., Interactive effects of phosphorus and light availability on early growth of maritime pine seedlings, Ann. For. Sci. 62 (2005) 575–583.

    Article  Google Scholar 

  8. Clements D.R., Peterson D., Prasad R., The biology of Canadian weeds — 112 — Ulex europaeus L., Can. J. Plant. Sci. 81 (2001) 325–337.

    Article  Google Scholar 

  9. Danso S.K.A., Hardarson G., Zapata F., Misconceptions and practical problems in the use of 15N soil enrichment techniques for estimating N2 fixation, Plant Soil 152 (1993) 25–52.

    Article  Google Scholar 

  10. Elabbadi K., Ismaili M., Materon L.A., Competion between Medicago truncutula and wheat for 15N labeled soil nitrogen and influence of phosphorus, Soil Biol. Biochem. 28 (1996) 83–88.

    Article  CAS  Google Scholar 

  11. Fisher R.F., Binkley D., Ecology and management of forest soils, Wiley and sons, New York, 2000.

    Google Scholar 

  12. Gentili F., Huss-Danell K., Local and systemic effects of phosphorus and nitrogen on nodulation and nodule function in Alnus incana, J. Exp. Bot. 54 (2003) 2757–2767.

    Article  PubMed  CAS  Google Scholar 

  13. Giller K.E., Amijee F., Brodrick S.J., Edje O.T., Environmental constraints to nodulation and nitrogen fixation of Phaseolus vulgaris L. in Tanzania. II. Response to N and P fertilizers and inoculation with Rhizobium, African Crop Sci. J. 6 (1998) 171–178.

    Google Scholar 

  14. Gunawardena S.F.B.N., Danso S.K.A., Zapata F., Phosphorus requirements and nitrogen accumulation by three mungbean (Vigna radiata (L.) Welzek) cultivars, Plant Soil 147 (1992) 267–274.

    Article  CAS  Google Scholar 

  15. Högberg P., 15N natural abundance in soil-plant systems, New Phytol. 137 (1997) 179–203.

    Article  Google Scholar 

  16. Laclau J.P., Ranger J., Deleporte P., Nouvellon Y., Saint-André L., Marlet S., Bouillet J.P., Nutrient cycling in a clonal stand of Eucalyptus and an adjacent savanna ecosystem in Congo. 3. Input-ouput budgets and consequences for the sustainability of the plantations, For. Ecol. Manage. 210 (2005) 375–391.

    Article  Google Scholar 

  17. O’Toole P., Cahalane D.G., Farrell E.P., Effects of phosphate fertilizer on biomass production and N2(C2H2) fixation by pot-grown Ulex gallii Planchon in a forest soil, Biol. Fertil. Soils 12 (1991) 177–181.

    Article  Google Scholar 

  18. Rastetter E.B., Vitousek P.M., Field C., Shaver G.R., Herbert D., Agren G.I., Resource optimization and symbiotic nitrogen fixation, Ecosystems 4 (2001) 369–388.

    Article  CAS  Google Scholar 

  19. Ribet J., Drevon J.J., The phosphorus requirement of N2-fixing and urea-fed Acacia mangium, New Phytol. 132 (1996) 383–390.

    Article  CAS  Google Scholar 

  20. Richardson R.G., Hill R.L., The biology of Australian weeds. 34. Ulex europaeus L., Plant Protection Quarterly 13 (1998) 46–58.

    Google Scholar 

  21. Sanginga N., Early growth and N2-fixation of leucaena and gliricidia at different levels of phosphorus application, Fertil. Res. 31 (1992) 165–173.

    Article  CAS  Google Scholar 

  22. Trichet P., Jolivet C., Arrouays D., Loustau D., Bert D., Ranger J., Le maintien de la fertilité des sols forestiers landais dans le cadre de la sylviculture intensive du pin maritime, Étude et Gestion des Sols 6 (1999) 197–214.

    Google Scholar 

  23. Trichet P., Loustau D., Lambrot C., Linder S., Manipulating nutrient and water availability in a maritime pine plantation: effects on growth, production, and biomass at canopy closure, Ann. For. Sci. 64 (2007).

  24. Warren C.R., McGrath J.F., Adams M.A., Differential effects of N, P and K on photosynthesis and partitioning of N in Pinus pinaster needles, Ann. For. Sci. 62 (2005) 1–8.

    Article  Google Scholar 

  25. Watt M.S., Clinton P.W., Whitehead D., Richardson B., Mason E.G., Leckie A.C., Above-ground biomass accumulation and nitrogen fixation of broom (Cytisus scoparius L.) growing with juvenile Pinus radiata on a dryland site, For. Ecol. Manage. 184 (2003) 93–104.

    Article  Google Scholar 

  26. Zeller B., Brechet C., Maurice J.-P, Le Tacon F., 13C and 15N isotopic fractionation in trees, soils and fungi in a natural forest stand and a Norway spruce plantation, Ann. For. Sci. 64 (2007).

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Authors and Affiliations

  1. UMR 1220 TCEM (INRA), BP 81, 33883, Villenave d’Ornon Cedex, France

    Xavier Cavard & Laurent Augusto

  2. UMR 1220 TCEM (ENITAB), 1 cour du Général de Gaulle, BP 201, 33175, Gradignan Cedex, France

    Etienne Saur

  3. UR 1263 EPHYSE (INRA), 69 route d’Arcachon, 33612, Cestas Cedex, France

    Pierre Trichet

Authors
  1. Xavier Cavard
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  2. Laurent Augusto
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  3. Etienne Saur
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  4. Pierre Trichet
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Correspondence to Laurent Augusto.

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Cavard, X., Augusto, L., Saur, E. et al. Field effect of P fertilization on N2 fixation rate of Ulex europaeus . Ann. For. Sci. 64, 875–881 (2007). https://doi.org/10.1051/forest:2007066

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  • DOI: https://doi.org/10.1051/forest:2007066

  • Ulex europaeus
  • symbiotic N2 fixation
  • 15N natural abundance
  • P fertilization
  • Pinus pinaster
  • Ulex europaeus
  • fixation symbiotique de l’azote
  • abondance naturelle en 15N
  • fertilisation en phosphore
  • Pinus pinaster