Skip to main content

Actual reproductive conflict during emergency queen rearing in Apis florea

Abstract

Unequal relatedness among workers in polyandrous honey bee colonies provides the potential for reproductive conflict during emergency queen rearing. Adult workers can increase their inclusive fitness by selectively rearing their full-sisters as queens. We investigated the paternity of emergency queens in two colonies of Apis florea using five microsatellite loci. In colony 1 there was no significant difference between the proportions of queens and workers in each patriline (P = 0.48). In contrast, the relative frequency of patrilines in colony 2 differed significantly between queens and workers (P = 0.03). More than a quarter of the queens reared in this colony were of a single patriline, suggesting that larvae were selected for rearing as queens non-randomly.

This is a preview of subscription content, access via your institution.

References

  • Beekman M., Ratnieks F.L.W. (2003) Power over reproduction in the social Hymenoptera, Philos. Trans. R. Soc. Lond. B. 358, 1741–1753.

    Article  Google Scholar 

  • Châline N., Arnold G., Papin C., Ratnieks F.L.W. (2003) Patriline differences in emergency queen rearing in the honey bee Apis mellifera, Insectes Soc. 50, 234–236.

    Article  Google Scholar 

  • Châline N., Martin S.J., Ratnieks F.L.W. (2005) Absence of nepotism towards imprisoned young queens during swarming of the honey bee, Behav. Ecol. 16, 403–409.

    Article  Google Scholar 

  • Estoup A., Solignac M., Cornuet J.-M. (1994) Precise assessment of the number of patrilines and of genetic relatedness in honey bee colonies, Proc. R. Soc. Lond. B 258, 1–7.

    Article  CAS  Google Scholar 

  • Estoup A., Solignac M., Harry M., Cornuet J.-M. (1993) Characterization of (GT)n and (CT)n microsatellites in two insect species: Apis mellifera and Bombus terrestris, Nucleic Acids Res. 21, 1427–1431.

    PubMed  Article  CAS  Google Scholar 

  • Hamilton W.D. (1964) The genetical evolution of social behaviour. I & II, J. Theor. Biol. 7, 1–52.

    PubMed  Article  CAS  Google Scholar 

  • Higgs J.S., Wattanachaiyingcharoen W., Oldroyd B.P. (2009) A scientific note on a genetically-determined colour morph of the dwarf honey bee, Apis andreniformis (Smith, 1858), Apidologie 40, 513–514.

    Article  Google Scholar 

  • Hughes W.O.H., Boomsma J.J. (2008) Genetic royal cheats in leaf-cutting ant societies, Proc. Nat. Acad. Sci. USA 105, 5150–5153.

    PubMed  Article  CAS  Google Scholar 

  • Koyama S., Harano K.-i., Hiroto T., Satoh T., Obara Y. (2007) Rearing of candidate queens by honeybee, Apis mellifera, workers (Hymenoptera: Apidae) is independent of genetic relatedness, Appl. Entomol. Zool. 42, 541–547.

    Article  Google Scholar 

  • Koyama S., Takagi T., Martin S.J., Yoshida T., Takahashi J. (2009) Absence of reproductive conflict during queen rearing in Apis cerana, Insectes Soc. 56, 171–175.

    Article  Google Scholar 

  • Nielsen R., Tarpy D.R., Reeve H.K. (2003) Estimating effective paternity number and the effective number of alleles in a population, Mol. Ecol. 12, 3157–3164.

    PubMed  Article  Google Scholar 

  • Oldroyd B.P., Clifton M.J., Wongsiri S., Rinderer T.E., Sylvester H.A., Crozier R.H. (1997) Polyandry in the genus Apis, particularly Apis andreniformis, Behav. Ecol. Sociobiol. 40, 17–26.

    Article  Google Scholar 

  • Oldroyd B.P., Gloag R.S., Even N., Wattanachaiyingcharoen W., Beekman M. (2008) Nest site selection in the open-nesting honeybee Apis florea, Behav. Ecol. Sociobiol. 62, 1643–1653.

    Article  Google Scholar 

  • Oldroyd B.P., Rinderer T.E., Buco S.M. (1990) Nepotism in the honey bee, Nature 346, 707–708.

    Article  Google Scholar 

  • Oldroyd B.P., Smolenski A.J., Cornuet J.-M., Wongsiri S., Estoup A., Rinderer T.E., Crozier R.H. (1996) Levels of polyandry and intracolonial genetic relationships in Apis dorsata (Hymenoptera: Apidae), Ann. Entomol. Soc. Am. 89, 276–283.

    Google Scholar 

  • Oldroyd B.P., Wongsiri S. (2006) Asian Honey Bees. Biology, Conservation and Human Interactions, Harvard University Press, Cambridge, Ma.

    Google Scholar 

  • Osborne K.E., Oldroyd B.P. (1999) Possible causes of reproductive dominance during emergency queen rearing by honeybees, Anim. Behav. 58, 267–272.

    PubMed  Article  Google Scholar 

  • Page R.E., Robinson G.E., Fondrk M.K. (1989) Genetic specialists, kin recognition and nepotism in honey-bee colonies, Nature 338, 576–579.

    Article  Google Scholar 

  • Palmer K.A., Oldroyd B.P. (2001) Mating frequency in Apis florea revisited (Hymenoptera: Apidae), Insectes Soc. 48, 40–43.

    Article  Google Scholar 

  • Pamilo P. (1993) Polyandry and allele frequency differences between the sexes in the ant Formica aquilonia, Heredity 70, 472–480.

    Article  Google Scholar 

  • Ratnieks F.L.W., Foster K.R., Wenseleers T. (2006) Conflict resolution in insect societies, Annu. Rev. Entomol. 51, 581–608.

    PubMed  Article  CAS  Google Scholar 

  • Ratnieks F.L.W., Reeve H.K. (1991) The evolution of queen-rearing nepotism in social Hymenoptera: effects of discrimination costs on swarming species, J. Evol. Biol. 4, 93–115.

    Article  Google Scholar 

  • Ratnieks F.L.W., Reeve H.K. (1992) Conflict in single-queen Hymenopteran societies: the structure of conflict, and processes that reduce conflict in advanced eusocial species, J. Theor. Biol. 158, 33–65.

    Article  Google Scholar 

  • Seeley T.D. (1985) Honeybee ecology, Princeton University Press, Princeton.

    Google Scholar 

  • Solignac M., Vautrin D., Loiseau A., Mougel F., Baudry E., Estoup A., Garnery L., Haberl M., Cornuet J.-M. (2003) Five hundred and fifty microsatellite markers for the study of the honeybee (Apis mellifera L.) genome, Mol. Ecol. Notes 3, 307–311.

    Article  CAS  Google Scholar 

  • Tarpy D.R., Gilley D.C., Seeley T.D. (2004) Levels of selection in a social insect: a review of conflict and cooperation during honey bee (Apis mellifera) queen replacement, Behav. Ecol. Sociobiol. 55, 213–523.

    Article  Google Scholar 

  • Tilley C.A., Oldroyd B.P. (1997) Unequal representation of subfamilies among queen and worker brood of queenless honey bee (Apis mellifera) colonies, Anim. Behav. 54, 1483–1490.

    PubMed  Article  Google Scholar 

  • Visscher P.K. (1993) A theoretical analysis of individual interests and intracolony conflict during swarming of honey bee colonies, J. Theor. Biol. 165, 191–212.

    Article  Google Scholar 

  • Walsh P.S., Metzger D.A., Higuchi R. (1991) Chelex (R)100 as a medium for simple extraction of DNA for PCR-based typing from forensic material, Biotechniques 10, 507.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Benjamin P. Oldroyd.

Additional information

Manuscript editor: Klaus Hartfelder

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Nanork, P., Low, P.A., Proft, K.M. et al. Actual reproductive conflict during emergency queen rearing in Apis florea . Apidologie 42, 206 (2011). https://doi.org/10.1051/apido/2010052

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1051/apido/2010052

Keywords

  • Apis florea
  • nepotism
  • emergency queen rearing
  • DNA microsatellites