Abstract
To identify the evolutionary lineage of honey bee colonies in Turkey, the mtDNA of 244 colonies from 20 locations was analyzed. Several polymorphic restriction sites showed that they belonged to the Mediterranean C lineage. DraI digestion of the CoxI—CoxII intergenic region produced four fragment patterns, one first seen in this study. From 37 colonies from 16 different locations in Turkey and two colonies from Iran, the intergenic region was sequenced. Previously, from among all honey bee populations of the C lineage, eight haplotypes had been described from this mtDNA region, three of which were found here. In addition, eight new haplotypes were found. A deletion in one of these haplotypes accounts for the novel DraI RFLP pattern. Most of the novel haplotypes were in a subgroup of lineage C, yet none of these had been found in previous studies of Turkish honey bees. The geographical distribution of some haplotypes suggests that they may be characteristic of subspecies native to Turkey.
Zusammenfassung
Die mitochondriale DNA (mtDNA) von 244 Honigbienenvölkern aus 20 Regionen der Türkei wurde hinsichtlich der folgenden Restriktionsprofile untersucht: (1) XbaI in der intergenen Region der Cytochromoxidase-Untereinheiten I und II (CoxI–CoxII), die einenTeil der CoxI Gene enthält; (2) BglII im Cytochrom-b Gen (Cyt-b); und (3) EcoRI im Gen der grossen ribosomalen RNA Untereinheit (lrRNA). Jeweils eine BglII, EcoRI und XbaI Schnittstelle lagen in den Cyt-b, lrRNA and CoxI Genen. Eine HincII Schnittstelle fehlte im CoxI Gen. Diese Polymorphismen sind charakteristisch für die mediterrane Linie (C) der Unterarten der Honigbiene. Für die intergene Region CoxI–CoxII fanden wir auch einenVerdau mittels DraI, wobei die vier Restriktionsfragmentmuster aus Längenunterschieden von eins bis zwei Basenpaaren in zwei der vier Fragmente resultierten. Drei dieser Muster waren bereits für A. m. ligustica, A. m. carnica und A. m. caucasica beschrieben worden und eine für Bienen aus der Türkei. Des weiteren fanden wir ein neues in dieser Untersuchung erstmals beschriebenes Muster.
Für Bienen von 37 Völkern aus 16 unterschiedlichen Regionen der Türkei und von zwei Völkern aus dem westlichyen Iran wurde die CoxI–CoxII intergene Region sequenziert. Die Sequenzen zeigten eine hohe Ähnlichkeit mit veröffentlichten Sequenzen von A. m. caucasica und A. m. anatoliaca. Vo n den bisher für Populationen der C-Linie beschriebenen acht Haplotypen in dieser mtDNA-Region fanden wir in unserer Untersuchung drei. Ausserdem fanden wir acht neue Haplotypen. Eine Deletion in einem dieser Haplotypen charakterisiert das neue DraI RFLP-Muster. Die geographische Verteilung der Haplotypen last darauf schliessen, dass sie entweder für A. m. anatoliaca oder A. m. meda charakteristisch sind.
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Manuscript editor: Walter S. Sheppard
The department where the study was conducted: University of Ankara, Faculty of Agriculture, Biometry & Genetics, 06110 Ankara-Turkey.
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Özdil, F., Yildiz, M.A. & Hall, H.G. Molecular characterization of Turkish honey bee populations (Apis mellifera) inferred from mitochondrial DNA RFLP and sequence results. Apidologie 40, 570–576 (2009). https://doi.org/10.1051/apido/2009032
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DOI: https://doi.org/10.1051/apido/2009032