Apidologie

, Volume 42, Issue 1, pp 103–116

The effects of four crop protection products on the morphology and ultrastructure of the hypopharyngeal gland of the European honeybee, Apis mellifera

  • Kevin Heylen
  • Bruno Gobin
  • Lutgarde Arckens
  • Roger Huybrechts
  • Johan Billen
Original Article

DOI: 10.1051/apido/2010043

Cite this article as:
Heylen, K., Gobin, B., Arckens, L. et al. Apidologie (2011) 42: 103. doi:10.1051/apido/2010043

Abstract

This study describes the impact of sublethal doses of 4 pesticides on size and morphology of the honeybee worker’s hypopharyngeal glands. This gland plays an essential role in brood care by young workers, and thus colony growth. Contaminating 7 day old caged bees, we sampled after 1 day and 1 week for Captan-, Imidacloprid- and Indoxacarb-treated bees and after 1, 2, 3, 4, 5 and 7 days for Fenoxycarb-treated bees. The glands’ acini 1 week post-treatment were all significantly smaller in treated bees. However, lightand electron microscopy showed hardly any difference between controls and gland cells, treated with Captan, Imidacloprid or Indoxacarb. Yet upon Fenoxycarb-treatment, acini showed a decrease in size, a granular texture and unorganized cytoplasm more quickly than normal. Indeed, after only 7 days, Fenoxycarb-treated glands displayed features typical of the onset of foraging in older bees.

Keywords

crop protection products hypopharyngeal gland honeybee histology 

Copyright information

© INRA, DIB-AGIB and Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Kevin Heylen
    • 1
    • 2
    • 3
  • Bruno Gobin
    • 4
    • 5
  • Lutgarde Arckens
    • 1
  • Roger Huybrechts
    • 3
  • Johan Billen
    • 2
  1. 1.Laboratory of Neuroplasticity and NeuroproteomicsKatholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Laboratory of EntomologyKatholieke Universiteit LeuvenLeuvenBelgium
  3. 3.Laboratory of Insect Physiology and Molecular EthologyKatholieke Universiteit LeuvenLeuvenBelgium
  4. 4.Zoology Department PcFruitSint-TruidenBelgium
  5. 5.Research Centre for Ornamental PlantsDestelbergenBelgium

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