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Apidologie

, Volume 42, Issue 1, pp 29–38 | Cite as

Effects of age, season and genetics on semen and sperm production in Apis mellifera drones

  • John W. Rhodes
  • Steven Harden
  • Robert Spooner-Hart
  • Denis L. AndersonEmail author
  • Gretchen Wheen
Original Article

Abstract

Adult drone honey bees from 4 Australian breeding lines were reared under similar conditions and examined for semen and sperm production when 14, 21 and 35 days old, during spring, summer and autumn. Almost half (40.5%) of all drones examined did not release any semen when manually everted. For those that released semen, the average volume released per drone was 1.09 μL (range 0.72 (±0.04)−1.12 (±0.04) μL) and the average number of sperms in the semen per drone was 3.63 × 106 (range 1.88 (±0.14)−4.11 (±0.17) × 106). The release of semen was dependent on breeding line and age (P < 0.05), but not on the rearing season. The volume of semen released per drone was dependent on season, age, and breeding line (P < 0.05), while the concentration of sperm in the semen was dependent on season and breeding line (P < 0.05). Hence our data indicate that genetics underpins the maturation of drone honey bees as well as the volume of semen they release and the concentration of sperm in that semen.

Keyword

Apis mellifera drones semen production sperm 

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Copyright information

© INRA, DIB-AGIB and Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • John W. Rhodes
    • 1
  • Steven Harden
    • 1
  • Robert Spooner-Hart
    • 2
  • Denis L. Anderson
    • 3
    Email author
  • Gretchen Wheen
    • 4
  1. 1.NSW Department of Primary Industries (DPI)Tamworth Agricultural InstituteCalalaAustralia
  2. 2.Centre for Plant and Food Science, Hawkesbury CampusUniversity of Western SydneyPenrith South DCAustralia
  3. 3.CSIRO EntomologyCanberraAustralia
  4. 4.RichmondAustralia

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