Agronomy for Sustainable Development

, Volume 31, Issue 2, pp 349–359 | Cite as

Decrease in rice aroma after application of growth regulators

  • Piebiep Goufo
  • Sugunya Wongpornchai
  • Xiangru Tang
Research Article


Aromatic rices (Oryza sativa L.) compared to the leading varieties are low-yielding, susceptible to lodging and prone to attack by a number of insect pests and diseases. Under these conditions, various agricultural chemicals such as fertilizers, pesticides and growth regulators have been used for their cultivation. Few investigations, however, have examined the influence of these chemicals on rice aroma and flavor. In this study, changes in rice aroma after treatment with gibberellic acid, paclobutrazol, 3-indole acetic acid, and a regulator mixture consisting of paclobutrazol, proline and zinc chloride were for the first time examined using two aromatic rice cultivars. Applications were carried out after 25% of panicles had emerged. We studied 12 odor-active compounds, extracted and identified using static headspace coupled with gas chromatography. At the concentrations tested, all treatments with growth regulators resulted in reduced aroma content that affected overall flavor. In a smelling evaluation, control samples were significantly higher in intensity than treated samples. The difference between the aromas of control and treated samples was largely related to 2-acetyl-1-pyrroline, the major rice aroma compound, and lipid oxidation volatiles. For instance, in the cultivar Guixiangzhan grown during the late season, gibberellic acid treatment decreased the content of 2-acetyl-1-pyrroline by 19%, 3-indole acetic acid by 9%, paclobutrazol by 22%, and the regulator mixture by 21% compared with the control. Similar trends were observed in the Peizaruanxiang cultivar, with decreases ranging from 10 to 24%. Our findings demonstrate that treatments with growth regulators inhibited the metabolic processes associated with the formation of volatile compounds.


aromatic rice paclobutrazol gibberellic acid 3-indole acetic acid 2-acetyl-1-pyrroline lipid-derived volatiles 


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  1. Bourgis F., Guyot R., Gherbi H., Tailliez E., Amabile I., Salse J., Lorieux M., Delseny M., Ghesquière A. (2008) Characterization of the major fragrance gene from an aromatic japonica rice and analysis of its diversity in Asian cultivated rice, Theor. Appl. Genet. 117, 353–368.PubMedCrossRefGoogle Scholar
  2. Bradbury L.M.T., Gillies S.A., Brushett D.J., Waters D.L.E., Henry R.J. (2008a). Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice, Plant Mol. Biol. 68, 439–449.PubMedCrossRefGoogle Scholar
  3. Bradbury M.L.T., Henry R.J., Waters D.L.E. (2008b) Flavor development in rice, in: Havkin-Frenkel D., Belanger F.C. (Eds.), Biotechnology in flavor production. Blackwell Publishing Ltd, Oxon, UK, pp. 130–146.CrossRefGoogle Scholar
  4. Buttery R.G., Turnbaugh J.G., Ling L.C. (1988) Contribution of volatiles to rice aroma, J. Agric. Food Chem. 36, 1006–1009.CrossRefGoogle Scholar
  5. Champagne E.T. (2008) Rice aroma and flavor: a literature review, Cereal Chem. 85, 445–454.CrossRefGoogle Scholar
  6. Chen S., Yang Y., Shi W., Ji Q., He F., Zhang Z., Cheng Z., Liu X., Xu M. (2008) Badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance, Plant Cell 20, 1850–1861.PubMedCrossRefGoogle Scholar
  7. Fitzgerald M.A., Hamilton N.R.S., Calingacion M.N., Verhoeven H.A., Butardo V.M. (2008) Is there a second fragrance gene in rice? Plant Biotechnol. J. 6, 416–423.PubMedCrossRefGoogle Scholar
  8. Goufo P., Duan M., Wongpornchai S., Tang X. (2010) Some factors affecting concentration of the aroma compound 2-acetyl-1-pyrroline in two fragrant rice cultivars grown in South China, Front. Agric. China 4, 1–9.CrossRefGoogle Scholar
  9. Huang T.C., Teng C.S., Chang J.L., Chuang H.S., Ho C.T., Wu M.L. (2008) Biosynthetic mechanism of 2-acetyl-1-pyrroline and its relationship with pyrroline-5-carboxylic acid and methylglyoxal in aromatic rice callus, J. Agric. Food Chem. 56, 7399–7404.PubMedCrossRefGoogle Scholar
  10. Juliano B.O., Villareal C.P. (1993) Grain quality evaluation of world rices, International Rice Research Institute, Manila, Philippines.Google Scholar
  11. Lam H.S., Proctor A. (2003) Milled rice oxidation volatiles and odor development, J. Food Sci. 68, 2676–2681.CrossRefGoogle Scholar
  12. Lançon J., Wery J., Rapidel B., Angokaye M., Gérardeaux E., Gaborel C., Ballo D., Fadegnon B. (2007) An improved methodology for integrated crop management systems, Agron. Sustain. Dev. 27, 101–110.CrossRefGoogle Scholar
  13. Loutfi K., Chlyah H. (1998) Vegetative multiplication of date palms from in vitro cultured inflorescences: effect of some growth regulator combinations and organogenetic potential of various cultivars, Agron. Sustain. Dev. 18, 573–580.Google Scholar
  14. Mander L.N. (2003) Twenty years of gibberellins research, Nat. Prod. Rep. 20, 49–69.PubMedCrossRefGoogle Scholar
  15. Maraval I., Mestres C., Pernin K., Ribeyre F., Boulanger R., Guichard E., Gunata Z. (2008) Odor-active compounds in cooked rice cultivars from Camargue (France) analyzed by GC-O and GC-MS, J. Agric. Food Chem. 56, 5291–5298.PubMedCrossRefGoogle Scholar
  16. Petrov M., Danzart M., Giampaoli P., Faure J., Richard H. (1996) Rice aroma analysis: discrimination between a scented and non-scented rice, Sci. Aliments 16, 347–360.Google Scholar
  17. Sawan Z.M., Sakr R.A. (1998) Effect of 1-naphthalene acetic acid concentrations and the number of applications on the yield components, yield and fibre properties of the Egyptian cotton (Gossypium barbadehse L.), Agron. Sustain. Dev. 18, 275–283.Google Scholar
  18. Sriseadka T., Wongpornchai S., Kitsawatpaiboon P. (2006) Rapid method for quantitative analysis of the aroma impact compound, 2-acetyl-1-pyrroline, in fragrant rice using automated headspace gas chromatography, J. Agric. Food Chem. 54, 8183–8189.PubMedCrossRefGoogle Scholar
  19. Sudria C., Palazon J., Cusido R., Bonfill M., Pinol M.T., Morales C. (2001) Effect of benzyladenine and indolebutyric acid on ultrastructure, glands formation, and essential oil accumulation in Lavandula dentata plantlets, Biol. Plant. 44, 1–6.CrossRefGoogle Scholar
  20. Suzuki Y., Ise K., Li C., Honda I., Iwai Y., Matsukura U. (1999) Volatile components in stored rice (Oryza sativa L.) varieties with and without lipoxygenase-3 in seeds, J. Agric. Food Chem. 47, 1119–1124.PubMedCrossRefGoogle Scholar
  21. Widjaja R., Craske J.D., Wootton M. (1996) Comparative studies on volatile components of non-fragrant and fragrant rices, J. Sci. Food Agric. 70, 151–161.CrossRefGoogle Scholar
  22. Wongpornchai S., Dumri K., Jongkaewwattana S., Siri B. (2004) Effects of drying methods and storage time on the aroma and milling quality of rice (Oryza sativa L.) cv. Khao Dawk Mali 105, Food Chem. 87, 407–414.CrossRefGoogle Scholar
  23. Yang D.S., Shewfelt R.L., Lee K.S., Kays S.J. (2008) Comparison of odor-active compounds from six distinctly different rice flavor types, J. Agric. Food Chem. 56, 2780–2787.PubMedCrossRefGoogle Scholar
  24. Yim K.O., Kwon Y.W., Bayer D.E. (1997) Growth responses and allocation of assimilates of rice seedlings by paclobutrazol and gibberellin treatment, J. Plant Growth Regul. 16, 35–41.CrossRefGoogle Scholar
  25. Yong I.K., Ji S.S., Nilda R.B., Tay E.H., Oksoo H., Baik H.C., Sunyo J., Ja O.G. (2003) Antioxidative enzymes offer protection from chilling damage in rice plants, Crop Sci. 43, 2109–2117.CrossRefGoogle Scholar
  26. Yoshihashi T.N., Nguyen T.T.H., Inatomi H. (2002) Precursors of 2- acetyl-l-pyrroline, a potent flavor compound of an aromatic rice variety, J. Agric. Food Chem. 50, 2001–2004.PubMedCrossRefGoogle Scholar

Copyright information

© INRA and Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Piebiep Goufo
    • 1
    • 2
  • Sugunya Wongpornchai
    • 3
  • Xiangru Tang
    • 1
  1. 1.Department of Crop Science and TechnologySouth China Agricultural UniversityGuangzhouP.R. China
  2. 2.Department of BiochemistryUniversity of Yaoundé IYaoundé FSCameroon
  3. 3.Department of ChemistryChiang Mai UniversityChiang MaiThailand

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