Abstract
Background
Cadmium (Cd)-induced testicular damage in relation to spermatogenesis has not been well studied. We studied the mechanism of Cd-induced testicular damage in a rat model of subchronic intoxication.
Methods
Male Sprague-Dawley rats were subcutaneously injected with 0.6 mg Cd/kg per day for 6 weeks. The concentration of Cd in urine, serum and testes was measured by using atomic absorption spectrophotometry. Testicular damage was evaluated by counting the spermatogonia (SG) and spermatocytes (SC) on one cut-surface of five seminiferous tubules in stages VII or VIII of spermatogenesis every week. The location of intratesticular cadmium was determined by using oxine-fluorescent cytochemistry.
Results
There were no differences in the testes/bodyweight ratio between the study and control groups. The concentration of Cd in the testes increased more than 100-fold that in serum after week 2, suggesting active testicular Cd accumulation (1–3 mg/g tissue). Cadmium accumulation was detected in SG and SC. The number of SG and SC diminished significantly in the study group (week 2: SG 74%, SC 90%; week 4: SG 47%, SC 75%; week 6: SG 30%, SC 54% of the control, respectively).
Conclusions
Cadmium accumulated in SG and SC, consequently reduced the number of these cells, and disturbed the spermatogenesis in this rat model of subchronic Cd intoxication. Therefore, the number of SG decreased in this rat model of subchronic Cd intoxication.
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References
Friberg L, Kjellstrom T, Nordberg GF. Cadmium. In: Friberg L, Nordberg GF, Vouk VB, eds. Handbook on the Toxicology of Metals, Vol. 2. New York: Elsevier, 1986; 130–184.
Samarawickrama GP. In: Webb M, ed. The Chemistry, Biochemistry and Biology of Cadmium. Amsterdam: Elsevier, 1979; 341–408.
Waalkes MP, Rehm S, Riggs CW et al. Cadmium carcinogenesis in male Wistar rats: dose-response analysis of tumor induction in the prostate and testes and at the injection site. Cancer Res 1988; 48: 4656–4663.
Xu C, Johnson JE, Singh PK et al. In vivo studies of cadmium-induced apoptosis in testicular tissue of the rat and its modulation by a chelating agent. Toxicology 1996; 197: 1–8.
Tanimoto A, Hamada T, Koide O. Cell death and regeneration of renal proximal tubular cells in rats with subchronic cadmium intoxication. Toxicol Pathol 1993; 21: 341–352.
Vesterberg O, Wrangskogh K. Determination of cadmium in urine by graphite-furnace atomic absorption spectroscopy. Clin Chem 1978; 24: 681–685.
Gavrieli Y, Shrman Y, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 1992; 119: 493–501.
Hamada T, Tanimoto A, Iwai S et al. Cytopathological changes induced by cadmium-exposure in canine proximal tubular cells: a cytochemical and ultrastructural study. Nephron 1994; 68: 104–111.
Nolan CV, Shaikh ZA. The vascular endothelium as a target tissue in acute cadmium toxidty. Life Sci 1986; 39: 1403–1409.
Suzuki JS, Kodama N, Molotkov A et al. Isolation and identification of metallothionein isoforms (MT-1 and MT-2) in the rat testis. Biochem J 1998; 334: 695–701.
Matsui H, Takahashi M. A novel quantitative morphometry of germ cells for the histopathological evaluation of rat testicular toxicity. J Toxicol Sci 1999; 24: 17–25.
Lafuente A, Marquez N, Perez-Lorenzo M et al. Pubertal and postpubertal cadmium exposure differentially affects the hypothalamic-pituitary-testicular axis function in the rat. Food Chem Toxicol 2000; 38: 913–923.
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Aoyagi, T., Ishikawa, H., Miyaji, K. et al. Cadmium-induced testicular damage in a rat model of subchronic intoxication. Reprod Med Biol 1, 59–63 (2002). https://doi.org/10.1046/j.1445-5781.2002.00010.x
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DOI: https://doi.org/10.1046/j.1445-5781.2002.00010.x