Ecological Research

, Volume 18, Issue 4, pp 431–441 | Cite as

Microbial biomass and abundance after forest fire in pine forests in Japan

  • Jhonamie A. MabuhayEmail author
  • Nobukazu Nakagoshi
  • Takao Horikoshi
Original Articles

Microbial characteristics of soil are being evaluated increasingly as sensitive indicators of soil health because of the clear relationship between microbial diversity, soil and plant quality and ecosystem sustainability. This study aimed to determine microbial carbon biomass and microbial abundance after fire to estimate the degree of damage, including the rate of recovery of micro-organisms, in each area. The study also aimed to establish relationships between microbial biomass and microbial abundance and the physico-chemical properties of the soil. The study was conducted in three different study areas in Hiroshima prefecture, one unburned area and two burnt areas (one immediately after and one 2 years after fire). anova showed a significant difference in microbial carbon biomass and microbial abundance among the study areas. Microbial carbon biomass and microbial abundance were highest in the unburned area, followed by the area burnt 2 years ago and lastly by the area studied immediately after fire. Carbon biomass was highly correlated with microbial abundance (r2 = 0.950). Carbon biomass and microbial abundance were shown to be significantly correlated to the soil’s physico-chemical properties, such as pH, moisture content, water-holding capacity and carbon : nitrogen (C : N) ratio. However, the C : N ratio was closely correlated to both carbon biomass and microbial abundance with r2 = 0.705 (P < 0.01) and r2 = 0.560 (P < 0.01), respectively.

Key words

abundance carbon biomass chloroform-fumigation extraction forest fire Hiroshima Japan 


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  1. Alexander M. (1961) Introduction to Soil Microbiology. Wiley, USA.Google Scholar
  2. Angara E. V., Nakagoshi N. & Nehira K. (2000) Twenty-one years post-fire succession in a small watershed on Etajima island, Hiroshima prefecture, southwestern Japan. Journal of International Development and Cooperation 6: 177–196.Google Scholar
  3. Anonymous (2000) Soil texture and particle size determination. Scholar
  4. Bauder J. (1999) When is organic matter built up or used up. Scholar
  5. Brookes P. C., Landman A., Pruden G. & Jenkinson D. S. (1985) Chloroform fumigation and the release of soil nitrogen; A rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biology and Biochemistry 17: 837–842.Google Scholar
  6. Childers G. W., Bancroft K. & Shaffer G. P. (1996) Bioremediation protocol for small scale oil spills in Louisiana marshes. Louisiana Oil Spill Coordinators Office/Office of the Governor Louisiana Oil Spill Research and Development Program OSRADP Technical Report Series 95-004.Google Scholar
  7. DeBano L. F., Eberlein G. E. & Dunn P. H. (1979) Effects of burning on chaparral soils. I. Soil nitrogen. Soil Science Society of America Journal 43: 504–509.Google Scholar
  8. Eldridge C. (1998) The effects of prescribed burns on microbial processes and microbial community composition in the soils of the Cape Cod National Seashore. Scholar
  9. Freckman D. W. (1994) Soil biodiversity: Its importance to ecosystem processes. In: Report from a workshop held at the Natural History Museum London, England. Scholar
  10. Gardeners Supply Company (2002) Building healthy soil: Improve your soil fertility and structure. Scholar
  11. Heal O. W., Struwe S. & Kjollen A. (1994) Diversity of Soil Biota and Ecosystem Function. Cambridge University Press, Cambridge.Google Scholar
  12. Mader S. S. (2001) Biology, 7th edn. The McGraw-Hill Company Inc., New York.Google Scholar
  13. Maier R. M., Pepper I. L. & Gerba C. P. (2000) Environmental Microbiology. Academic Press, San DiegoGoogle Scholar
  14. Munsell A. H. (1976) Munsell Book of Color. Macbeth, Baltimore.Google Scholar
  15. Nakagoshi N. (2001) Forest fire and management in pine forest ecosystems in Japan. Hikobia 13: 301–311.Google Scholar
  16. Oyama M. & Takehara H. (1997) Revised Standard Soil Color Charts, 20th edn. Nihon Shikiken Gigyo, Tokyo.Google Scholar
  17. Tateishi T. & Horikoshi T. (1995) Microbial biomass in the soils of burned and unburned Japanese Red Pine forests in the Setouchi District, Western Japan. Bulletin of the Japanese Society of Microbial Ecology 10: 9–20.Google Scholar
  18. Tateishi T., Horikoshi T., Tsubota H. & Takahashi F. (1989a) Microbial biomass and microflora in the soils at burned and unburned Japanese red pine forests. Bulletin of the Japanese Society of Microbial Ecology 4: 77–87.Google Scholar
  19. Tateishi T., Horikoshi T., Tsubota H. & Takahashi F. (1989b) Application of the chloroform fumigation-incubation method to the estimation of soil microbial biomass in burned and unburned Japanese red pine forests. FEMS Microbiology Ecology 62: 163–172.Google Scholar
  20. Vance E. D., Brookes P. C. & Jenkinson D. S. (1987) An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry 19: 703–707.CrossRefGoogle Scholar
  21. Weaver J. E. & Rowland N. W. (1952) Effects of excessive natural mulch on development, yield and structure of native grassland. The Botanical Gazette 114: 1–19.Google Scholar
  22. Wells C. G., Campbell R. E., DeBano L. F., Lewis C. E., Frederickson R. L., Franklin E. C., Froelich R. C. & Dunn P. H. (1979) Effects of fire on soil. USDA Forest Service General Technical Report Wo-7. USDA Forest Service, Washington.Google Scholar
  23. Wright H. A. & Bailey A. W. (1982) Fire Ecology: United States and Southern Canada. Wiley, New York.Google Scholar

Copyright information

© Ecological Society of Japan 2003

Authors and Affiliations

  • Jhonamie A. Mabuhay
    • 1
    Email author
  • Nobukazu Nakagoshi
    • 1
  • Takao Horikoshi
    • 2
  1. 1.Graduate School for International Development and CooperationHiroshima UniversityKagamiyamaHigashi-HiroshimaJapan
  2. 2.Department of Environmental Sciences, Faculty of Integrated Arts and SciencesHiroshima UniversityHigashi-HiroshimaJapan

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