Notes and references
N. Eevers, J. D. Van Hamme, E. M. Bottos, N. Weyens and J. Vangronsveld, Draft Genome Sequence of Methylobacterium radiotolerans, a DDE-Degrading and Plant Growth-Promoting Strain Isolated from Cucurbita pepo., Genome Announc., 2015, 3, e00488–e00415.
M. Cordovana, A. Deni, M. Kostrzewa, M. Abdalla and S. Ambretti, First report of Methylobacterium radiotolerans bacteraemia identified by MALDI-TOF mass spectrometry, New Microbes New Infect., 2019, 30, 100546.
S. Altschul, T. L. Madden, A. A. Schäffer, J. Zhang, Z. Zhang, W. Miller and D. J. Lipman, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res., 1997, 25, 3389–3402.
G. Gourinchas, S. Etzl and A. Winkler, Bacteriophytochromes – from informative model systems of phytochrome function to powerful tools in cell biology, Curr. Opin. Struct. Biol., 2019, 57, 72–83.
S.-Y. Park and J. R. H. Tame, Seeing the light with BLUF proteins, Biophys. Rev., 2017, 9, 169–176.
A. Losi, K. H. Gardner and A. Möglich, Blue-Light Receptors for Optogenetics, Chem. Rev., 2018, 118, 10659–10709.
T. Kottke, A. Xie, D. S. Larsen and W. D. Hoff, Photoreceptors Take Charge: Emerging Principles for Light Sensing, Annu. Rev. Biophys., 2018, 47, 291–313.
X. Shu, V. Lev-Ram, T. J. Deerinck, Y. Qi, E. B. Ramko, M. W. Davidson, Y. Jin, M. H. Ellisman and R. Y. Tsien, A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms, PLoS Biol., 2011, 9, e1001041.
P. R. Ogilby, Singlet oxygen: there is indeed something new under the sun, Chem. Soc. Rev., 2010, 39, 3181.
J. Torra, C. Lafaye, L. Signor, S. Aumonier, C. Flors, X. Shu, S. Nonell, G. Gotthard and A. Royant, Tailing miniSOG: structural bases of the complex photophysics of a flavinbinding singlet oxygen photosensitizing protein, Sci. Rep., 2019, 9, 2428.
S. Endres, M. Wingen, J. Torra, R. Ruiz-González, T. Polen, G. Bosio, N. L. Bitzenhofer, F. Hilgers, T. Gensch, S. Nonell, K.-E. Jaeger and T. Drepper, An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria., Sci. Rep., 2018, 8, 15021.
M. Westberg, M. Etzerodt and P. R. Ogilby, Rational design of genetically encoded singlet oxygen photosensitizing proteins, Curr. Opin. Struct. Biol., 2019, 57, 56–62.
M. Westberg, M. Bregnhøj, M. Etzerodt and P. R. Ogilby, No Photon Wasted: An Efficient and Selective Singlet Oxygen Photosensitizing Protein, J. Phys. Chem. B, 2017, 121, 9366–9371.
R. Ruiz-González, A. L. Cortajarena, S. H. Mejias, M. Agut, S. Nonell and C. Flors, Singlet Oxygen Generation by the Genetically Encoded Tag miniSOG, J. Am. Chem. Soc., 2013, 135, 9564–9567.
F. M. Pimenta, R. L. Jensen, T. Breitenbach, M. Etzerodt and P. R. Ogilby, Oxygen-Dependent Photochemistry and Photophysics of “MiniSOG,” a Protein-Encased Flavin, Photochem. Photobiol., 2013, 89, 1116–1126.
A. Losi, C. Mandalari and W. Gärtner, The Evolution and Functional Role of Flavin-based Prokaryotic Photoreceptors, Photochem. Photobiol., 2015, 91, 1021–1031.
M. Wingen, K.-E. Jaeger, T. Gensch and T. Drepper, Novel Thermostable Flavin-binding Fluorescent Proteins from Thermophilic Organisms, Photochem. Photobiol., 2017, 93, 849–856.
M. Westberg, M. Bregnhøj, M. Etzerodt and P. R. Ogilby, Temperature Sensitive Singlet Oxygen Photosensitization by LOV-Derived Fluorescent Flavoproteins, J. Phys. Chem. B, 2017, 121, 2561–2574.
B. J. Fritz, K. Matsui, S. Kasai and A. Yoshimura, Triplet lifetimes of some flavins, Photochem. Photobiol., 1987, 45, 539–541.
P. F. Heelis, The photophysical and photochemical properties of flavins (isoalloxazines), Chem. Soc. Rev., 1982, 11, 15.
B. Kopka, K. Magerl, A. Savitsky, M. D. Davari, K. Röllen, M. Bocola, B. Dick, U. Schwaneberg, K.-E. Jaeger and U. Krauss, Electron transfer pathways in a light, oxygen, voltage (LOV) protein devoid of the photoactive cysteine, Sci. Rep., 2017, 7, 13346.
A. S. Halavaty and K. Moffat, N- and C-Terminal Flanking Regions Modulate Light-Induced Signal Transduction in the LOV2 Domain of the Blue Light Sensor Phototropin 1 from Avena sativa, Biochemistry, 2007, 46, 14001–14009.
A. Möglich and K. Moffat, Structural Basis for Light-dependent Signaling in the Dimeric LOV Domain of the Photosensor YtvA, J. Mol. Biol., 2007, 373, 112–126.
M. Westberg, L. Holmegaard, F. M. Pimenta, M. Etzerodt and P. R. Ogilby, Rational Design of an Efficient, Genetically Encodable, Protein-Encased Singlet Oxygen Photosensitizer, J. Am. Chem. Soc., 2015, 137, 1632–1642.
J. Torra, A. Burgos-Caminal, S. Endres, M. Wingen, T. Drepper, T. Gensch, R. Ruiz-González and S. Nonell, Singlet oxygen photosensitisation by the fluorescent protein Pp2FbFP L30 M, a novel derivative of Pseudomonas putida flavin-binding Pp2FbFP, Photochem. Photobiol. Sci., 2015, 14, 280–287.
C. Martí, O. Jürgens, O. Cuenca, M. Casals and S. Nonell, Aromatic ketones as standards for singlet molecular oxygen O2(1Δg) photosensitization. Time-resolved photoacoustic and near-IR emission studies, J. Photochem. Photobiol., A, 1996, 97, 11–18.
A. Losi, E. Polverini, B. Quest and W. Gärtner, First Evidence for Phototropin-Related Blue-Light Receptors in Prokaryotes, Biophys. J., 2002, 82, 2627–2634.
N. Yadav and A. Nath Yadav, Biodiversity and biotechnological applications of novel plant growth promoting methylotrophs, J. Appl. Biotechnol. Bioeng., 2018, 5, 342–344.
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Electronic supplementary information (ESI) available: Fig. S1-S6, materials and methods. See DOI: 10.1039/c9pp00328b
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Consiglieri, E., Xu, Q., Bregnhøj, M. et al. Single mutation in a novel bacterial LOV protein yields a singlet oxygen generator. Photochem Photobiol Sci 18, 2657–2660 (2019). https://doi.org/10.1039/c9pp00328b
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DOI: https://doi.org/10.1039/c9pp00328b