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Photodynamic inactivation of S. aureus with a water-soluble curcumin salt and an application to cheese decontamination

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Abstract

In this study, the optimal parameters for the photodynamic inactivation (PDI) of Staphylococcus aureus in bacterial suspensions and in cheese were assessed using a water-soluble curcumin salt as the photosensitizer (PS). The in vitro study aimed at finding the optimal concentration and light dose to promote S. aureus photokilling. Four main groups were proposed: CONTROL (L−C−), LIGHT (L+C−), CUR (L−C+) and PDI (L+C+). A fixed light dose (LED, 450 ± 10 nm, 10 J cm−2) was applied using four different PS concentrations (0.75, 1.0, 1.5 and 3.0 mg mL−1). The dose also varied from 10–100 J cm−2 for a fixed concentration. High inactivation rates were observed for all light doses, with a maximum reduction of 7.58 log10at 100 J cm−2 (p ≪ 0.05). Saturation of the PDI effect was observed after a 10 minute illumination time, as well as a slight decrease in the S. aureus population for increasing illumination times in the L+C− group. As an application, the concentration showing the best decontamination performance in vitro (0.75 mg mL–1) was applied to decontaminate cheese in loco. PDI in two types of coalho cheese, a rennet-coagulated cheese commonly consumed in Brazil, was investigated. The results showed no significant inactivation in unpasteurized cheese, but a 4.34 log10 reduction for t > 5 min in pasteurized specimens. In conclusion, the present PDI-catalyzed curcumin photosensitizer inactivated S. aureus at statistically significant levels in vitro, in pasteurized cheese, but not in unpasteurized specimens.

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Authors and Affiliations

  1. Universidade Federal de Alagoas, Instituto de Física, Lourival Melo Mota Ave., s/n., Maceió-AL, 57072-900, Brazil

    Robenilson Ferreira dos Santos, Beliato Santana Campos, Francisco de Assis M. G. Rego Filho, Pedro Valentim dos Santos & Maria Tereza de Araujo

  2. Universidade Federal de Alagoas, Faculdade de Nutrição, Lourival Melo Mota Ave., s/n., Maceió-AL, 57072-900, Brazil

    Juliana de Oliveira Moraes, Amália Luisa Ivo Albuquerque & Maria Cristina Delgado da Silva

  3. Instituto Federal de Alagoas, Campus Piranhas, Sergipe Ave., s/n., Piranhas-AL, 57460-000, Brazil

    Robenilson Ferreira dos Santos & Francisco de Assis M. G. Rego Filho

  4. Instituto Federal de Alagoas, Campus Murici. Conj. Residencial Astolfo Lopes, s/n. Cidade Alta, Murici-AL, 57820-000, Brazil

    Juliana de Oliveira Moraes

  5. Serviço Nacional de Aprendizagem Comercial, Comendador Francisco Amorim Leão Ave., 240A. Pinheiro, Maceió-AL, 57057-780, Brazil

    Amália Luisa Ivo Albuquerque

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  1. Robenilson Ferreira dos Santos
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  2. Beliato Santana Campos
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  3. Francisco de Assis M. G. Rego Filho
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  4. Juliana de Oliveira Moraes
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  6. Maria Cristina Delgado da Silva
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  7. Pedro Valentim dos Santos
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  8. Maria Tereza de Araujo
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Correspondence to Francisco de Assis M. G. Rego Filho.

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Ferreira dos Santos, R., Campos, B.S., Filho, F.M.G.R. et al. Photodynamic inactivation of S. aureus with a water-soluble curcumin salt and an application to cheese decontamination. Photochem Photobiol Sci 18, 2707–2716 (2019). https://doi.org/10.1039/c9pp00196d

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