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Water-dispersible glycosylated poly (2,5’-thienylene)porphyrin-based nanoparticles for antibacterial photodynamic therapy

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Abstract

Here we report the preparation of water-dispersible glycosylated poly(2,5’-thienylene)porphyrin based nanoparticles by a nanoprecipitation method and demonstrate the application of these nanoparticles in antibacterial photodynamic therapy. The diameter of the nanoparticles is in the range of 50–80 nm and the resulting nanoparticles are stable in water without precipitation at least for a month. They have high singlet oxygen efficiency and display light-triggered biocidal activity against both Gram negative bacteria (Escherichia coli, E. coli) and Gram positive bacteria (Bacillus subtilis, B. subtilis). Upon white light irradiation for 10 min with a flux of 22 mW cm−2 of the E. coli suspension incubated with NPs (18 μg mL−1), a killing efficiency of 99% is achieved, whereas in the dark the effect is recorded as only around 8%.

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Authors and Affiliations

  1. Department of Chemistry, Bilkent University, 06800, Ankara, Turkey

    Rehan Khan, Melis Özkan, Aisan Khaligh & Dönüs Tuncel

  2. Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, 06800, Turkey

    Rehan Khan, Melis Özkan, Aisan Khaligh & Dönüs Tuncel

Authors
  1. Rehan Khan
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  2. Melis Özkan
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  3. Aisan Khaligh
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  4. Dönüs Tuncel
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Corresponding author

Correspondence to Dönüs Tuncel.

Additional information

Electronic supplementary information (ESI) available: Full synthetic scheme for PTTP-Glu-Ac, synthetic procedure for PTTP, 1H, 13C-NMR spectra of PTTP, 1H, 13C-NMR, FT-IR, UV-Vis, PL spectra of PTTP-Glu-Ac, time-dependent decrease of absorbance spectra for DPBF with NPs, minimum inhibitory concentration plots of NPs against E. coli in the dark and under light, plate photographs for NPs against B. subtilis on YTD agar plate in the dark and under light. See DOI: 10.1039/c8pp00470f

These authors contributed equally.

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Khan, R., Özkan, M., Khaligh, A. et al. Water-dispersible glycosylated poly (2,5’-thienylene)porphyrin-based nanoparticles for antibacterial photodynamic therapy. Photochem Photobiol Sci 18, 1147–1155 (2019). https://doi.org/10.1039/c8pp00470f

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  • DOI: https://doi.org/10.1039/c8pp00470f

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