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Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium

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Abstract

The bis-retinoid N-retinylidene-N-retinylethanolamine (A2E) is one of the major components of lipofuscin, a fluorescent material that accumulates with age in the lysosomes of the retinal pigment epithelium (RPE) of the human eye. Lipofuscin, as well as A2E, exhibit a range of cytotoxic properties, which are thought to contribute to the pathogenesis of degenerative diseases of the retina such as Age-related Macular Degeneration. Consistent with such a pathogenic role, high levels of lipofuscin fluorescence are found in the central area of the human RPE, and decline toward the periphery. Recent reports have however suggested a surprising incongruence between the distributions of lipofuscin and A2E in the human RPE, with A2E levels being lowest in the central area and increasing toward the periphery. To appraise such a possibility, we have quantified the levels of A2E in the central and peripheral RPE areas of 10 eyes from 6 human donors (ages 75–91 years) with HPLC and UV/VIS spectroscopy. The levels of A2E in the central area were on average 3–6 times lower than in peripheral areas of the same eye. Furthermore, continuous accumulation of selected ions (CASI) imaging mass spectrometry showed the presence of A2E in the central RPE, and at lower intensities than in the periphery. We have therefore corroborated that in human RPE the levels of A2E are lower in the central area compared to the periphery. We conclude that the levels of A2E cannot by themselves provide an explanation for the higher lipofuscin fluorescence found in the central area of the human RPE.

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Abbreviations

A2E:

N-Retinylidene-N-retinylethanolamine

AMD:

Age-related macular degeneration

AUC:

Area under the curve

RPE:

Retinal pigment epithelium

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Authors and Affiliations

  1. Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA

    Leopold Adler IV, Nicholas P. Boyer, Zsolt Ablonczy, Rosalie K. Crouch & Yiannis Koutalos

  2. Mass Spectrometry Research Center, Vanderbilt School of Medicine, Nashville, TN, USA

    David M. Anderson, Jeffrey M. Spraggins & Kevin L. Schey

  3. Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA

    Anne Hanneken

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  1. Leopold Adler IV
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  2. Nicholas P. Boyer
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  9. Yiannis Koutalos
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Correspondence to Yiannis Koutalos.

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Adler, L., Boyer, N.P., Anderson, D.M. et al. Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium. Photochem Photobiol Sci 14, 1983–1990 (2015). https://doi.org/10.1039/c5pp00156k

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