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Determination of pyruvic acid concentration using a bioluminescence system from Photobacterium leiognathi

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Abstract

A novel, highly sensitive and selective bacterial luminescence method for the detection of pyruvic acid (PA) is reported here. This method is based on a reaction system catalyzed by lactate dehydrogenase (LDH) with the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence system in vitro. The reduced nicotinamide adenine dinucleotide (NADH) involved in the LDH reaction system could be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration was exhibited within the range of 0.00014–0.001 mol l-1, and the pyruvic acid detection limit was found to be 8.537 × 10-5 mol l-1. This method was successfully applied to the detection of PA in quail serum with a good recovery of over 70%.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

    Guanhua Xuan, Xiaodong Lu, Jingxue Wang, Hong Lin & Huihui Liu

Authors
  1. Guanhua Xuan
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  2. Xiaodong Lu
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  3. Jingxue Wang
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  4. Hong Lin
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  5. Huihui Liu
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Correspondence to Jingxue Wang.

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Xuan, G., Lu, X., Wang, J. et al. Determination of pyruvic acid concentration using a bioluminescence system from Photobacterium leiognathi. Photochem Photobiol Sci 14, 1163–1168 (2015). https://doi.org/10.1039/c5pp00118h

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