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The role of duplex stability for wavelength-shifting fluorescent DNA probes: energy transfer vs. exciton interactions in DNA “traffic lights”

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Abstract

Exciton interactions between thiazole orange and thiazole red as nucleotide substitutes in DNA hairpins interfere with efficient energy transfer and fluorescence color change as readout. This interference can be tuned by two structural parameters that control the hairpin duplex stability.

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Authors and Affiliations

  1. Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany

    Sebastian Barrois, Samantha Wörner & Hans-Achim Wagenknecht

Authors
  1. Sebastian Barrois
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  2. Samantha Wörner
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  3. Hans-Achim Wagenknecht
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Correspondence to Hans-Achim Wagenknecht.

Additional information

Electronic supplementary information (ESI) available: Details of synthesis, DNA preparation and purification, and spectroscopic measurements. See DOI: 10.1039/c4pp00153b

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Barrois, S., Wörner, S. & Wagenknecht, HA. The role of duplex stability for wavelength-shifting fluorescent DNA probes: energy transfer vs. exciton interactions in DNA “traffic lights”. Photochem Photobiol Sci 13, 1126–1129 (2014). https://doi.org/10.1039/c4pp00153b

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  • DOI: https://doi.org/10.1039/c4pp00153b

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