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In vitro sensing of Cu+ through a green fluorescence rise of pyranine

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Abstract

Pyranine as a new class of fluorescent chemosensor for the Cu+ ion is reported. The probe is capable of discriminating ranges of cations from the Cu+ ion, even in competing environment. The dye displayed a rapid fluorescence response (t1/2 = 1.66 min) towards the Cu+ ion, and the micromolar detection limit enabled the detection of the ion in environmental samples. The observed stoichiometry of complexation between pyranine and Cu+ was 2: 1. Interestingly, the sensing characteristic was specific to only neutral pH. A metal-to-ligand charge-transfer (MLCT)-based mechanism of sensing was proposed based on electron spin resonance (EPR), Raman spectroscopic and cyclic voltammetric studies.

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Authors and Affiliations

  1. Department of Chemistry, Indian Institute of Science Education and Research, Pune, India

    Tanmoy Saha, Abhigyan Sengupta, Partha Hazra & Pinaki Talukdar

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  1. Tanmoy Saha
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  2. Abhigyan Sengupta
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Correspondence to Pinaki Talukdar.

Additional information

† Electronic supplementary information (ESI) available: Additional experimental procedures, fluorescence spectra and cyclic voltammetry. See DOI: 10.1039/c4pp00097h

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Saha, T., Sengupta, A., Hazra, P. et al. In vitro sensing of Cu+ through a green fluorescence rise of pyranine. Photochem Photobiol Sci 13, 1427–1433 (2014). https://doi.org/10.1039/c4pp00097h

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  • DOI: https://doi.org/10.1039/c4pp00097h

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