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Anomalous diffusion of TePixD and identification of the photoreaction product

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Abstract

TePixD is a blue-light sensor protein from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TePixD Tll0078). Although the photochemistry has been examined, so far the photoproduct remains unknown. We have measured the diffusion coefficient (D) of TePixD in the dark by dynamic light scattering and have discovered a very peculiar diffusion property; the decamer oligomer has a larger D than that of the pentamer. Furthermore, D of the pentamer was found to be very close to that of the TePixD decamer photoreaction product. In order to investigate this reaction further, elution profiles of size-exclusion chromatography were measured under dark and illuminated conditions at low (40 µM) and high (1.1 mM) TePixD concentrations. On the basis of these results, we have concluded that the main photoreaction of the TePixD decamer is the dissociation into the pentamer. The secondary structure change associated with this reaction was found to be minor according to circular dichroism analysis.

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Authors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan

    Kunisato Kuroi, Keisuke Tanaka & Masahide Terazima

  2. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo, 153-8902, Japan

    Koji Okajima & Masahiko Ikeuchi

  3. Research Institute for Advanced Science and Technology, Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan

    Koji Okajima & Satoru Tokutomi

Authors
  1. Kunisato Kuroi
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  2. Keisuke Tanaka
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  3. Koji Okajima
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  4. Masahiko Ikeuchi
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  5. Satoru Tokutomi
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  6. Masahide Terazima
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Correspondence to Masahide Terazima.

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Kuroi, K., Tanaka, K., Okajima, K. et al. Anomalous diffusion of TePixD and identification of the photoreaction product. Photochem Photobiol Sci 12, 1180–1186 (2013). https://doi.org/10.1039/c3pp25434h

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  • DOI: https://doi.org/10.1039/c3pp25434h

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