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Development of multiplexed analysis for the photocatalytic activities of nanoparticles in aqueous suspension

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Abstract

A multiplexed assay technique to measure the photocatalytic activity (PCA) of nanoparticles (NPs) in aqueous suspension was developed based on the observation of TiO2 NPs-photocatalytic oxidation rate of NADH by monitoring the fluorescence intensities. 96 sample solutions of a small volume (< 150 μL) could be assayed in a single run without separation of NPs within 15 min. PCA values can be measured with high sensitivity and low experimental uncertainties through the observation at various concentrations of photocatalyst, substrate, aqueous protons and pH buffer ions in a short measurement time.

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Authors and Affiliations

  1. Center for Nanobio Convergence, Korea Research Institute of Standards and Science, 209 Gajeong-Ro, Yuseong-Gu, 305-340, Daejeon, Korea

    No Ah Lee, Soo Jin Kim, Bong-Jae Park & Nam Woong Song

  2. Center for Nano-Characterization, Korea Research Institute of Standards and Science, 305-340, Daejon, Korea

    Hyun Min Park

  3. Molecular/Nano Photochemistry & Photonics Lab., Department of Chemistry, Chungnam National University, 99 Daehak-Ro, Yuseong-Gu, Dae-jeon, 305-764, Korea

    No Ah Lee & Minjoong Yoon

  4. BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology and Nanobiotechnology Major School of Engineering, University of Science and Technology, 111 Gwahang-Ro, Yuseong-Gu, 305-806, Daejeon, Korea

    Bong Hyun Chung

Authors
  1. No Ah Lee
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  2. Soo Jin Kim
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  3. Bong-Jae Park
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  4. Hyun Min Park
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  5. Minjoong Yoon
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  6. Bong Hyun Chung
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  7. Nam Woong Song
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Corresponding author

Correspondence to Minjoong Yoon.

Additional information

Electronic supplementary information (ESI) available: Procedures for material preparation and spectroscopic measurement. Concentration dependence of substrate fluorescence intensity and photocatalytic activity. Photo-oxidation kinetics for the explanation of pH dependent PCA. See 10.1039/c1pp05244f

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Lee, N.A., Kim, S.J., Park, BJ. et al. Development of multiplexed analysis for the photocatalytic activities of nanoparticles in aqueous suspension. Photochem Photobiol Sci 10, 1979–1982 (2011). https://doi.org/10.1039/c1pp05244f

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  • DOI: https://doi.org/10.1039/c1pp05244f

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