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Photocatalytic inactivation of bacteriophages by TiO2-coated glass plates under low-intensity, long-wavelength UV irradiation

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Abstract

The International Organization for Standardization (ISO) was used to evaluate antibacterial activity by titanium dioxide (TiO2) photocatalysis since 2006. We evaluated photocatalytic inactivation of Qβ and T4 bacteriophages induced by low-intensity, long-wavelength ultraviolet A (UVA; 0.1 mW cm−2 and 0.001 mW cm−2) irradiation on a TiO2-coated glass plate using the ISO methodology. The results indicated that both bacteriophages were inactivated at 0.001 mW cm−2 UVA. The intensity of UV light, including long-wavelength light (UVA), is very low in an actual indoor environment. Thus, TiO2 photocatalysis can be beneficial for inactivating viruses in an indoor environment. Experiments using qPCR and bovine serum albumin degradation assume that viral inactivation is caused by outer viral protein disorder and not by viral RNA reduction by reactive oxygen species produced during TiO2 photocatalysis. Furthermore, we showed that the ISO methodology for standard testing of antibacterial activity by TiO2 photocatalysis can be applied to assess antiviral activity.

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Authors and Affiliations

  1. Photocatalyst Group, Kanagawa Academy of Science and Technology, KSP West Building, 614, 3-2-1, Sakado, Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan

    Hitoshi Ishiguro, Ryuichi Nakano, Yanyan Yao, Jitsuo Kajioka, Akira Fujishima & Yoshinobu Kubota

  2. Department of Urology, Yokohama City University Graduate School of Medicine, 3-9, Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan

    Hitoshi Ishiguro, Yanyan Yao & Yoshinobu Kubota

  3. Kitasato Research Center of Environment Sciences, 1-15-1, Kitasato, Sagamihara, Kanagawa, 228-8555, Japan

    Ryuichi Nakano

  4. Department of Applied Chemistry, Faculty of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Kayano Sunada, Masafumi Minoshima & Kazuhito Hashimoto

  5. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan

    Kazuhito Hashimoto

Authors
  1. Hitoshi Ishiguro
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  2. Ryuichi Nakano
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  3. Yanyan Yao
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  4. Jitsuo Kajioka
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  5. Akira Fujishima
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  6. Kayano Sunada
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  7. Masafumi Minoshima
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  8. Kazuhito Hashimoto
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  9. Yoshinobu Kubota
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Correspondence to Hitoshi Ishiguro.

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Ishiguro, H., Nakano, R., Yao, Y. et al. Photocatalytic inactivation of bacteriophages by TiO2-coated glass plates under low-intensity, long-wavelength UV irradiation. Photochem Photobiol Sci 10, 1825–1829 (2011). https://doi.org/10.1039/c1pp05192j

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  • DOI: https://doi.org/10.1039/c1pp05192j

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