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Influence of DNA-binding on the photochromic equilibrium of a chromene derivative

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Abstract

The photochromic properties of a chromene derivative, which contains a positively charged substituent, were investigated in the presence of ct DNA. Upon irradiation in the absence of DNA the colored quinoid open form is highly unstable and rapidly reverts back to the initial closed form (bleaching). In the presence of DNA the open form is significantly stabilized, presumably because of the association with the macromolecule. The kinetics of the bleaching reaction of the bound ligand were determined and employed to estimate the binding constant.

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Authors and Affiliations

  1. A.N. Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences, 28 Vavilova St., 119991, Moscow, Russian Federation

    Sergey V. Paramonov & Olga A. Fedorova

  2. Centre Interdisciplinaire de Nanoscience de Marseille (CINaM, CNRS UPR 3118), Aix-Marseille Université, 163 Av. de Luminy, 13288, Marseille, France

    Vladimir Lokshin

  3. Organic Chemistry II, University of Siegen, Adolf-Reichwein-Str. 2, 57068, Siegen, Germany

    Heiko Ihmels

Authors
  1. Sergey V. Paramonov
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  2. Vladimir Lokshin
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  3. Heiko Ihmels
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  4. Olga A. Fedorova
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Correspondence to Sergey V. Paramonov.

Additional information

Electronic supplementary information (ESI) available: Details of the synthesis and kinetic scheme. See DOI: 10.1039/c1pp05094j

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Paramonov, S.V., Lokshin, V., Ihmels, H. et al. Influence of DNA-binding on the photochromic equilibrium of a chromene derivative. Photochem Photobiol Sci 10, 1279–1282 (2011). https://doi.org/10.1039/c1pp05094j

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  • DOI: https://doi.org/10.1039/c1pp05094j

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