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Photodynamic inactivation of Escherichia coli by Ru(II) complexes

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Abstract

[Ru(II)(bpy)2(dppn)]2+ (bpy = 2,2′-bipyridine, dppn = 4,5,9,16-tetraazadibenzo[a,c]naphthacene) was found to be able to photoinactivate Gram-negative Escherichia coli efficiently, showing the potential of transition-metal complexes as photosensitizers in the field of photodynamic antimicrobial chemotherapy (PACT).

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References

  1. H. C. Neu, The crisis in antibiotic resistance, Science, 1992, 257, 1064.

    Article  CAS  PubMed  Google Scholar 

  2. M. Wainwright, Photodynamic antimicrobial chemotherapy (PACT), J. Antimicrob. Chemother., 1998, 42, 13

    Article  CAS  PubMed  Google Scholar 

  3. S. Noimark, C. W. Dunnill, M. Wilson and I. P. Parkin, The role of surfaces in catheter-associated infections, Chem. Soc. Rev., 2009, 38, 3435.

    Article  CAS  PubMed  Google Scholar 

  4. M. R. Hamblin and T. Hasan, Photodynamic therapy: a new antimicrobial approach to infectious disease?, Photochem. Photobiol. Sci., 2004, 3, 436.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Z. Malik, H. Ladan and Y. Nitzan, Photodynamic inactivation of Gram-negative bacteria: problems and possible solutions, J. Photochem. Photobiol., B, 1992, 14, 262

    Article  CAS  Google Scholar 

  6. R. Bonnett, D. G. Buckley, T. Burrow, A. B. B. Galia, B. Saville and S. P. Songca, Photobactericidal materials based on porphyrins and phthalocyanines, J. Mater. Chem., 1993, 3, 323.

    Article  CAS  Google Scholar 

  7. M. Merchat, G. Bertoloni, P. Giacomoni, A. Villanueva and G. Jori, Meso-substituted cationic porphyrins as efficient photosensitizers of gram-positive and gram-negative bacteria, J. Photochem. Photobiol., B, 1996, 32, 153

    Article  CAS  Google Scholar 

  8. M. R. Hamblin, D. A. O’Donnell, N. Murthy, K. Rajagopalan, N. Michaud, M. E. Sherwood and T. Hasan, Polycationic photosensitizer conjugates: effects of chain length and Gram classification on the photodynamic inactivation of bacteria, J. Antimicrob. Chemother., 2002, 49, 941

    Article  CAS  PubMed  Google Scholar 

  9. B. Hager, W. S. L. Strauss and H. Falk, Cationic hypericin derivatives as novel agents with photobactericidal activity: synthesis and photodynamic inactivation of propionibacterium acnes, Photochem. Photobiol., 2009, 85, 1201

    Article  CAS  PubMed  Google Scholar 

  10. D. O. Frimannsson, M. Grossi, J. Murtagh, F. Paradisi and D. F. O’Shea, Light induced antimicrobial properties of a brominated boron difluoride (BF2) chelated tetraarylazadipyrromethene, J. Med. Chem., 2010, 53, 7337.

    Article  CAS  PubMed  Google Scholar 

  11. K. E. Erkkila, D. T. Odom and J. K. Barton, Recognition and reaction of metallointercalators with DNA, Chem. Rev., 1999, 99, 2777

    Article  CAS  PubMed  Google Scholar 

  12. W. H. Ang and P. J. Dyson, Classical and non-classical ruthenium-based anticancer drugs: Towards targeted Chemotherapy, Eur. J. Inorg. Chem., 2006, 4003

    Google Scholar 

  13. B. M. Zeglis, V. C. Pierre and J. K. Barton, Metallointercalators and metalloinsertors, Chem. Commun., 2007, 4565.

    Google Scholar 

  14. H. Y. Mei and J. K. Barton, Tris(tetramethylphenanthroline) ruthenium(II): a chiral probe that cleaves A-DNA conformations, Proc. Natl. Acad. Sci. U. S. A., 1988, 85, 1339

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Y. Liu, R. Hammitt, D. A. Lutterman, R. P. Thummel and C. Turro, Marked differences in light-switch behavior of Ru(II) complexes possessing a tridentate DNA intercalating ligand, Inorg. Chem., 2007, 46, 6011

    Article  PubMed  Google Scholar 

  16. Y. Liu, R. Hammitt, D. A. Lutterman, R. P. Thummel and C. Turro, Ru(II) complexes of new tridentate ligands: unexpected high yield of sensitized 1O2, Inorg. Chem., 2009, 48, 375

    Article  CAS  PubMed  Google Scholar 

  17. Q. X. Zhou, F. Yang, W. H. Lei, J. R. Chen, C. Li, Y. J. Hou, X. C. Ai, J. P. Zhang, X. S. Wang and B. W. Zhang, Ruthenium(II) terpyridyl complexes exhibiting DNA photocleavage: The role of the substituent on monodentate ligand, J. Phys. Chem. B, 2009, 113, 11521

    Article  CAS  PubMed  Google Scholar 

  18. Y. Sun, L. E. Joyce, N. M. Dickson and C. Turro, Efficient DNA photocleavage by [Ru(bpy)2(dppn)]2+ with visible light, Chem. Commun., 2010, 46, 2426.

    Article  CAS  Google Scholar 

  19. T. Gianferrara, I. Bratsos and E. Alessio, A categorization of metal anticancer compounds based on their mode of action, Dalton Trans., 2009, 7588.

    Google Scholar 

  20. Q. X. Zhou, W. H. Lei, J. R. Chen, C. Li, Y. J. Hou, X. S. Wang and B. W. Zhang, A new heteroleptic ruthenium(II) polypyridyl complex with long-wavelength absorption and high singlet-oxygen quantum yield, Chem. Eur. J., 2010, 16, 3157

    Article  CAS  PubMed  Google Scholar 

  21. Q. X. Zhou, W. H. Lei, Y. Sun, J. R. Chen, C. Li, Y. J. Hou, X. S. Wang and B. W. Zhang, [Ru(bpy)3-n(dpb)n]2+: Unusual photophysical property and efficient DNA photocleavage activity, Inorg. Chem., 2010, 49, 4729.

    Article  CAS  PubMed  Google Scholar 

  22. C. A. Puckett and J. K. Barton, Methods to explore cellular uptake of ruthenium complexes, J. Am. Chem. Soc., 2007, 129, 46.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. A. A. Abdel-Shafi, P. D. Beer, R. J. Mortimer and F. Wilkinson, Photosensitized generation of singlet oxygen from vinyl linked benzocrown-ether–bipyridyl ruthenium(II) complexes, J. Phys. Chem. A, 2000, 104, 192.

    Article  CAS  Google Scholar 

  24. E. Reddi, M. Ceccon, G. Valduga, G. Jori, J. C. Bommer, F. Elisei, L. Latterini and U. Mazzucato, Photophysical properties and antibacterial activity of meso-substituted cationic porphyrins, Photochem. Photobiol., 2002, 75, 462.

    Article  CAS  PubMed  Google Scholar 

  25. X. Q. Guo, F. N. Castellano, L. Li and J. R. Lakowicz, A longlifetime Ru(II) metal–ligand complex as a membrane probe, Biophys. Chem., 1998, 71, 51

    Article  CAS  PubMed  Google Scholar 

  26. M. Ardhammar, P. Lincoln and B. Nordén, Ligand substituents of ruthenium dipyridophenazine complexes sensitively determine orientation in liposome membrane, J. Phys. Chem. B, 2001, 105, 11363.

    Article  CAS  Google Scholar 

  27. A. E. Friedman, J. C. Chambron, J. P. Sauvage, N. J. Turro and J. K. Barton, A molecular light switch for DNA: Ru(bpy)2(dppz)2+, J. A m. Chem. Soc., 1990, 112, 4960.

    Article  CAS  Google Scholar 

  28. C. A. Strassert, M. Otter, R. Q. Albuquerque, A. Höne, Y. Vida, B. Maier and L. D. Cola, Photoactive hybrid nanomaterial for targeting, labeling, and killing antibiotic-resistant bacteria, Angew. Chem., Int. Ed., 2009, 48, 7928

    Article  CAS  Google Scholar 

  29. C. Xing, Q. Xu, H. Tang, L. Liu and S. Wang, Conjugated polymer/porphyrin complexes for efficient energy transfer and improving light-activated antibacterial activity, J. Am. Chem. Soc., 2009, 131, 13117.

    Article  CAS  PubMed  Google Scholar 

  30. G. Valduga, B. Breda, G. M. Giacometti, G. Jori and E. Reddi, Photosensitization of wild and mutant strains of Escherichia coli by meso-tetra (N-methyl-4-pyridyl)porphine, Biochem. Biophys. Res. Commun., 1999, 256, 84.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China

    Wanhua Lei, Qianxiong Zhou, Guoyu Jiang, Baowen Zhang & Xuesong Wang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Wanhua Lei & Guoyu Jiang

Authors
  1. Wanhua Lei
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  2. Qianxiong Zhou
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  3. Guoyu Jiang
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  4. Baowen Zhang
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  5. Xuesong Wang
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Corresponding author

Correspondence to Xuesong Wang.

Additional information

Electronic supplementary information (ESI) available: Experimental section; Table S1; Fig. S1–S7. See DOI: 10.1039/c0pp00275e

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Lei, W., Zhou, Q., Jiang, G. et al. Photodynamic inactivation of Escherichia coli by Ru(II) complexes. Photochem Photobiol Sci 10, 887–890 (2011). https://doi.org/10.1039/c0pp00275e

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  • DOI: https://doi.org/10.1039/c0pp00275e

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