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Methacrylate monomers lower the level of reduced glutathione and increase the in vitro sensitivity of cells to optical radiation

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Abstract

Induction of cell death by optical radiation in the wavelength range 350–500 nm was significantly increased by commonly used methacrylate monomers, not mediated by absorption of radiation by the methacrylate monomers, but through a mechanism involving rapid reduction in the level of glutathione (GSH).

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References

  1. S. Vainiotalo, A. Zitting, S. Jacobsson, J. Nickels, H. Koskinen, H. Savolainen, Toxicity of polymethylmethacrylate thermodegradation products, Arch. Toxicol., 1984, 55, 137–142.

    Article  CAS  Google Scholar 

  2. E. B. Roll, J. E. Dahl, G. Runningen, E. Morisbak, In vitro cell death induced by irradiation and chemicals relevant for dental applications; dose-response and potentiation effects, Eur. J. Oral Sci., 2004, 112, 273–279.

    Article  CAS  Google Scholar 

  3. T. Christensen, E. Morisbak, H. H. Tønnesen, E. M. Bruzell, In vitro photosensitization initiated by camphorquinone and phenyl propanedione in dental polymeric materials, J. Photochem. Photobiol., B, 2010, 100, 128–134.

    Article  CAS  Google Scholar 

  4. H. Schweikl, G. Spagnuolo, G. Schmalz, Genetic and cellular toxicology of dental resin monomers, J. Dent. Res., 2006, 85, 870–877.

    Article  CAS  Google Scholar 

  5. R. Becher, H. M. Kopperud, R. H. Al, J. T. Samuelsen, E. Morisbak, H. J. Dahlman, E. M. Lilleaas, J. E. Dahl, Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts, Dent. Mater., 2006, 22, 630–640.

    Article  CAS  Google Scholar 

  6. J. T. Samuelsen, J. E. Dahl, S. Karlsson, E. Morisbak, R. Becher, Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK, Dent. Mater., 2007, 23, 34–39.

    Article  CAS  Google Scholar 

  7. R. R Miller, J. A. Ayres, L. W. Rampy, M. J. McKenna, Metabolism of acrylate esters in rat tissue homogenates, Fundam. Appl. Toxicol., 1981, 1, 410–414.

    Article  CAS  Google Scholar 

  8. L. Stanislawski, E. Soheili-Majd, A. Perianin, M. Goldberg, Dental restorative biomaterials induce glutathione depletion in cultured human gingival fibroblast: protective effect of N-acetyl cysteine, J. Biomed. Mater. Res., 2000, 51, 469–474.

    Article  CAS  Google Scholar 

  9. J. Engelmann, G. Leyhausen, D. Leibfritz, W. Geurtsen, Effect of TEGDMA on the intracellular glutathione concentration of human gingival fibroblasts, J. Biomed. Mater. Res., 2002, 63, 746–751.

    Article  CAS  Google Scholar 

  10. T. J. McCarthy, E. P. Hayes, C. S. Schwartz, G. Witz, The Reactivity of selected acrylate esters toward glutathione and deoxyribonucleosides in vitro: Structure–activity relationships, Fundam. Appl. Toxicol., 1994, 22, 543–548.

    Article  CAS  Google Scholar 

  11. S. Fujisawa, Y. Kadoma, Prediction of the reduced glutathione (GSH) reactivity of dental methacrylate monomers using NMR spectra - Relationship between toxicity and GSH reactivity, Dent. Mater. J., 2009, 28, 722–729.

    Article  CAS  Google Scholar 

  12. J. T. Samuelsen, H. M. Kopperud, J. A. Holme, I. S. Dragland, T. Christensen, J. E. Dahl, In vitro toxicity and glutathione-complex formation in cells exposed to 2-hydroxyethyl-methacrylate, J. Biomed. Mater. Res. A, submitted.

  13. J. B Mitchell, A. Russo, The role of glutathione in radiation and drug induced cytotoxicity, Br. J. Cancer, 1987, 55, suppl. VIII, 96–104.

    CAS  Google Scholar 

  14. A. C. Miller, B. W. Henderson, The influence of cellular glutathione content on cell survival following photodynamic treatment in vitro, Radiat. Res., 1986, 107, 83–94.

    Article  CAS  Google Scholar 

  15. J. Volk, G. Leyhausen, S. Dogan, W. Geurtsen, Additive effects of TEGDMA and hydrogenperoxide on the cellular glutathione content of human gingival fibroblasts, Dent. Mater., 2007, 23, 921–926.

    Article  CAS  Google Scholar 

  16. R. M. Tyrrell, M. Pidoux, Endogenous glutathione protects human skin fibroblasts against the cytotoxic action of UVB, UVA and near-visible radiations, Photochem. Photobiol., 1986, 44, 561–564.

    Article  CAS  Google Scholar 

  17. R. K. Clayton, Role of H2O2 in the photooxidative killing of blue-green mutants of Rhodopseudomonas spheroides, J. Bacteriol., 1961, 82, 314–315.

    Article  CAS  Google Scholar 

  18. R. J. Wang, J. D. Stoien, F. Landa, Lethal effect of near-ultraviolet irradiation on mammalian cells in culture, Nature, 1974, 247, 43–45.

    Article  CAS  Google Scholar 

  19. R. J Wang, B. T. Nixon, Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light, In Vitro, 1978, 14, 715–722.

    Article  CAS  Google Scholar 

  20. B. S. Rosenstein, J. M. Ducore, S. W. Cummings, The mechanism of bilirubin-photosensitized DNA strand breakage in human cells exposed to phototherapy light, Mutat. Res., 1983, 112, 397–406.

    CAS  PubMed  Google Scholar 

  21. E. B. Roll, N. Jacobsen, A. Hensten-Pettersen, Health hazards associated with curing light in the dental clinic, Clin. Oral. Invest., 2004, 8, 113–117.

    Google Scholar 

  22. D. O. Quissell, K. A. Barzen, D. C. Gruenert, R. S. Redman, J. M. Camden, J. T. Turner, Development and characterization of SV40 immortalized rat submandibular acinar cell lines, In Vitro Cell. Dev. Biol.: Anim., 1997, 33, 164–173.

    Article  CAS  Google Scholar 

  23. T. Haukvik, E. M. Bruzell, S. Kristensen, H. H. Tønnesen, Photokilling of bacteria by curcumin in different aqueous preparations. Studies on curcumin and curcuminoids XXXVII, Pharmazie, 2009, 64, 666–673.

    CAS  PubMed  Google Scholar 

  24. Y. J. Lee, E. Shacter, Oxidative stress inhibits apoptosis in human lymphoma cells, J. Biol. Chem., 1999, 274, 19792–19798.

    Article  CAS  Google Scholar 

  25. N. S. Kosower, E. M. Kosower, G. L. Newton, H. M. Ranney, Bimane fluorescent labels: labeling of normal human red cells under physiological conditions, Proc. Natl. Acad. Sci. U. S. A., 1979, 76, 3382–3386.

    Article  CAS  Google Scholar 

  26. M. Noda, J. C. Wataha, J. B. Lewis, M. Kaga, P. E. Lockwood, R. L. Messer, H. Sano, Dental adhesive compounds alter glutathione levels but not glutathione redox balance in human THP-1 monocytic cells, J. Biomed. Mater. Res. B Appl. Biomater., 2005, 73, 308–314.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. NIOM - Nordic Institute of Dental Materials, P.O. Box 70, NO-1305, Haslum, Norway

    Terje Christensen & Ellen M. Bruzell

  2. Norwegian Radiation Protection Authority, Section for Non-Ionising Radiation, P.O. Box 55, NO-1332, ØsterÅs, Norway

    Terje Christensen

Authors
  1. Terje Christensen
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  2. Ellen M. Bruzell
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Correspondence to Terje Christensen.

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Christensen, T., Bruzell, E.M. Methacrylate monomers lower the level of reduced glutathione and increase the in vitro sensitivity of cells to optical radiation. Photochem Photobiol Sci 9, 1597–1600 (2010). https://doi.org/10.1039/c0pp00240b

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  • DOI: https://doi.org/10.1039/c0pp00240b

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