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Characterization of new fluorescent labels for ultra-high resolution microscopy

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Abstract

Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus offer additional independent fluorescence-based information. However, although providing a useful mechanism for photo-induced switching, the triplet state often appears as a precursor state for photobleaching, which potentially can limit its usefulness. In this work, a set of rhodamine and pyronindyes, modified by substitution of heavy atoms and nitrogen within or close to the central xanthene unit of the dyes, were investigated with respect to their triplet state dynamics and photostabilities, under conditions relevant for ultra-high resolution microscopy. Out of the dyes investigated, in particular the rhodamine and pyronindyes with a sulfur atom replacing the central oxygen atom in the xanthene unit were found to meet the requirements for ultra-high resolution microscopy, combining a prominent triplet state yield with reasonable photostability.

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Authors and Affiliations

  1. Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-10691, Stockholm, Sweden

    Andriy Chmyrov & Jerker Widengren

  2. ATTO-TEC GmbH, Am Eichenhang 50, D-57076, Siegen, Germany

    Jutta Arden-Jacob

  3. Department of Chemistry, University of Siegen, D-57068, Siegen, Germany

    Alexander Zilles & Karl-Heinz Drexhage

Authors
  1. Andriy Chmyrov
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  2. Jutta Arden-Jacob
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  3. Alexander Zilles
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  4. Karl-Heinz Drexhage
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  5. Jerker Widengren
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Chmyrov, A., Arden-Jacob, J., Zilles, A. et al. Characterization of new fluorescent labels for ultra-high resolution microscopy. Photochem Photobiol Sci 7, 1378–1385 (2008). https://doi.org/10.1039/b810991p

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  • DOI: https://doi.org/10.1039/b810991p

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