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Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging

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Abstract

The fluorescence lifetime image of HeLa cells expressing an enhanced green fluorescent protein (EGFP)-fusion protein changes under stress, which allows noninvasive determination of the status of individual cells.

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Authors and Affiliations

  1. Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo, 060-0812, Japan

    Takakazu Nakabayashi, Issei Nagao, Masataka Kinjo & Nobuhiro Ohta

  2. Faculty of Advanced Life Science, Hokkaido University, Sapporo, 060-0810, Japan

    Masataka Kinjo

  3. Lab of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki, 444-8787, Japan

    Yumiko Aoki & Minoru Tanaka

  4. Division of Biological Science, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan

    Yumiko Aoki

Authors
  1. Takakazu Nakabayashi
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  2. Issei Nagao
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  3. Masataka Kinjo
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  4. Yumiko Aoki
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  5. Minoru Tanaka
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  6. Nobuhiro Ohta
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Corresponding author

Correspondence to Nobuhiro Ohta.

Additional information

Electronic supplementary information (ESI) available: Material and methods. See DOI: 10.1039/b805032e

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Nakabayashi, T., Nagao, I., Kinjo, M. et al. Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging. Photochem Photobiol Sci 7, 671–674 (2008). https://doi.org/10.1039/b805032e

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  • DOI: https://doi.org/10.1039/b805032e

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