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CCD imaging of basal bioluminescence in larval fireflies: clues on the anatomic origin and evolution of bioluminescence

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Abstract

The anatomic and biochemical origin of beetle bioluminescence is still poorly understood. Through CCD imaging we report that larvae and pupae of the Brazilian fireflies Aspisoma lineatum and Cratomorphus sp emit a continuous weak glow throughout the entire body during all stages. This luminescence is especially developed after feeding, ecdysis and in the pupal stage, gradually disappearing as the cuticle becomes sclerotized and the adult emerges. This weak glow arises from the fat body, which consists of small lobes spread all over the body cavity. According to their pigmentation, these lobes can be divided in whitish and pinkish, and display different luciferase isozymes. Morphological studies suggest that the jelly-like ventral lanterns in the 8th abdominal segment evolved from these white lobes, providing a rationale for the widespread location of lanterns in larvae of different bioluminescent beetles. The biological and biochemical function of this weak diffuse bioluminescence is discussed in the context of the larval life-history.

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References

  1. V. R. Viviani, The origin, diversity and structure function relationships of insect luciferases, Cell. Mol. Life Sci., 2002, 59, 1833–1850.

    Article  CAS  Google Scholar 

  2. M. A. Branham, J. W. Wenzel, The evolution of bioluminescence in Cantharoids (Coleoptera: Elateroidea), Florida Entomol., 2000, 84 4, 565.

    Article  Google Scholar 

  3. J. M. Bassot, Les cellules lumineuses du coleoptere Phengodes>. Extrait de Recherces Biologiques Contemporareus. Imp. Vagner 4 Trim. Nancy, France, 1974.

    Google Scholar 

  4. J. B. Buck, The anatomy and physiology of the light organs in fireflies, Ann. N. Y. Acad. Sci., 1948, 49, 397–483.

    Article  CAS  Google Scholar 

  5. H. Ghiradella, The anatomy of light production: the fine structure of the firefly lantern, Microscopic Anatomy of Invertebrates, 1998, 11A, 363–381.

    Google Scholar 

  6. M. A. Branham, J. W. Wenzel, The origin of photic behavior and evolution of sexual communication in fireflies, Cladistics, 2003, 23, 1–22.

    Article  Google Scholar 

  7. W. N. Hess, Origin and development of the light organs of Photuris pennsylvanica DeGeer, J. Morphol., 1922, 36, 245–263.

    Article  Google Scholar 

  8. K. N. Smalley, D. E. Tarwater, T. L. Davidson, Localization of fluorescent compounds in the firefly light organ, J. Histochem. Cytochem., 1980, 28, 323–329.

    Article  CAS  Google Scholar 

  9. C. H. Hanna, T. A. Hopkins, J. Buck, Peroxisomes of the firefly, J. Ultrastr. Res., 1976, 57, 150–162.

    Article  CAS  Google Scholar 

  10. L. G. Strause, M. DeLuca, J. F. Case, Biochemical and morfological changes accompanying organ development in the firefly, Photuris pennsylvanica, J. Insect Physiol., 1979, 25, 339–347.

    Article  CAS  Google Scholar 

  11. R. F. Chapmann, The Insects, Cambridge University Press, Cambridge, UK, 1998.

    Book  Google Scholar 

  12. V. R. Viviani, Fireflies (Coleoptera: Lampyridae) from Southeastern Brazil: Habitats, life history, and bioluminescence, Anna. Entomol. Soc. Am., 2001, 94, 129–145.

    Article  Google Scholar 

  13. C. Costa, Genus Pyrophorus 3. Life History, larva and Pupa of Pyrophorus punctatissimus Blanchard (Coleoptera: Elateridae), Papeis Avulsos de Zoologia, 1970, 23, 69–76.

    Google Scholar 

  14. Y. Oba, M. Sato, M. Ojika, S. Inouye, Enzymatic and genetic characterization of firefly luciferase and Drosophila CG6178 as a fatty acyl-CoA synthetase, Biosci., Biotechnol., Biochem., 2005, 69, 819–828.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brazil

    Viviani V. R., F. M. Okawachi, V. Scorsato & F. C. Abdalla

  2. Laboratório de Bioluminescência e Biotecnologia, Departamento de Farmácia, UNISO, Brazil

    Viviani V. R.

Authors
  1. Viviani V. R.
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  2. F. M. Okawachi
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  3. V. Scorsato
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  4. F. C. Abdalla
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Correspondence to Viviani V. R..

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Viviani, V.R., Okawachi, F.M., Scorsato, V. et al. CCD imaging of basal bioluminescence in larval fireflies: clues on the anatomic origin and evolution of bioluminescence. Photochem Photobiol Sci 7, 448–452 (2008). https://doi.org/10.1039/b718016k

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  • DOI: https://doi.org/10.1039/b718016k

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