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Suppression of contact hypersensitivity after repeated exposures of humans to low doses of solar simulated radiation

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Abstract

Although it is generally recognised that UV radiation (UVR) can induce suppression of contact hypersensitivity (CHS) in human subjects, most protocols to date have not tested the effect of low daily doses of solar simulated radiation (SSR). In the present study, healthy individuals, divided into four groups each consisting of approximately 34 subjects, were whole-body irradiated with 1.2 standard erythema doses of SSR for 2, 10 or 30 consecutive days, or were unirradiated. They were sensitised with diphenylocyclopropenone (DPCP) on one exposed body site 24 h after the final UVR. The occurrence and severity of the primary allergic response were noted, and both parameters were shown to be significantly lowered in the group irradiated for 30 days compared with the unirradiated group. Elicitation of CHS was undertaken 3 weeks after the sensitisation, using a range of concentrations of DPCP on a UV-protected body site. The extent of the CHS at 48 h was assessed by the clinical score, by an erythema meter and by histological examination of a biopsy taken from the site challenged with one selected concentration of DPCP. Although erythema and pigmentation did not differ between the groups, a significant negative correlation was found between the clinical CHS score and the number of days of UV exposure, at the lowest challenge dose of DPCP. In addition a significant negative correlation was revealed between the intensity of spongiosis (intraepidermal oedema and vesicles, as evaluated by histology) and the number of days of UV exposure. Thus small daily doses of SSR induce suppression of CHS in human subjects and the effect is cumulative, indicating that there is no adaptation to the immunomodulating effects of UVR, at least over the test period of 30 days.

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References

  1. J. Garseen, H. Van Loveren, Effects of ultraviolet exposure on the immune system, Crit. Rev. Immunol., 2001, 21, 359–397.

    Google Scholar 

  2. G. I. Harrison, A. R. Young, Ultraviolet radiation-induced erythema in human skin, Methods, 2002, 28, 14–19.

    Article  CAS  Google Scholar 

  3. M. S. Duthie, I. Kimber I, M. Norval, The effects of ultraviolet radiation on the human immune system, Br. J. Dermatol., 1999, 140, 995–1009.

    Article  CAS  Google Scholar 

  4. F. R. de Gruijl, J. Longstreth, M. Norval, A. P. Cullen, H. Slaper, M. L. Kripke, Y. Takizawa, J. van der Leun, Health effects from stratospheric ozone depletion and interactions with climate change, Photochem. Photobiol. Sci., 2003, 2, 16–28.

    Article  Google Scholar 

  5. S. Kodali, S. Beissert and R. D. Granstein, Physiology and pathology of skin photoimmunology, in Skin Immune System, ed. J. D. Bos, CRC Press, Boca Raton, FL, 3rd edn., 2004, pp. 457–474.

  6. G. J. Clydesdale, G. W. Dandie, H. K. Muller, Ultraviolet light induced injury: immunological and inflammatory effects, Immunol. Cell Biol., 2001, 79, 547–568.

    Article  CAS  Google Scholar 

  7. D. L. Damian, G. M. Halliday, Measurement of ultraviolet radiation-induced suppression of recall contact and delayed-type hypersensitivity in humans, Methods, 2002, 28, 34–45.

    Article  CAS  Google Scholar 

  8. B. L. Diffey, Sources and measurement of ultraviolet radiation, Methods, 2002, 28, 4–13.

    Article  CAS  Google Scholar 

  9. D. A. Kelly, S. L. Walker, J. M. McGregor, A. R. Young, A single exposure of solar simulated radiation suppresses contact hypersensitivity responses both locally and systemically in humans: quantitative studies with high frequency ultrasound, J. Photochem. Photobiol., B, 1998, 44, 130–142.

    Article  CAS  Google Scholar 

  10. D. L. Damian, R. S. Barnetson, G. M. Halliday, Effects of low-dose ultraviolet radiation on in vivo human cutaneous recall responses, Australas. J. Dermatol., 2001, 42, 161–167.

    Article  CAS  Google Scholar 

  11. D. A. Kelly, P. T. Seed, A. R. Young, S. L. Walker, A commercial sunscreen’s protection against ultraviolet radiation-induced immunosuppression is more than 50% lower than protection against sunburn in humans, J. Invest. Dermatol., 2003, 120, 65–71.

    Article  CAS  Google Scholar 

  12. E. D. Baron, A. Fourtanier, D. Compan, C. Medaisko, K. D. Cooper, S. R. Stevens, High ultraviolet A protection affords greater immune protection confirming that ultraviolet A contributes to photoimmunosuppression in humans, J. Invest. Dermatol., 2003, 121, 869–875.

    Article  CAS  Google Scholar 

  13. P. Wolf, C. Hoffmann, F. Quehenberger, S. Grinschgl, H. Kerl, Immune protection factors of chemical sunscreens measured in the local contact hypersensitivity model in humans, J. Invest. Dermatol., 2003, 121, 1080–1087.

    Article  CAS  Google Scholar 

  14. D. A. Kelly, A. R. Young, J. M. McGregor, P. T. Seed, C. S. Potten, S. L. Walker, Sensitivity to sunburn is associated with susceptibility to ultraviolet radiation-induced suppression of cutaneous cell-mediated immunity, J. Exp. Med., 2000, 191, 561–566.

    Article  CAS  Google Scholar 

  15. B. L. Diffey, C. T. Jansen, F. Urbach, H. C. Wulf, The standard erythema dose: a new photobiological concept, Photodermatol. Photoimmunol. Photomed., 1997, 13, 64–66.

    Article  CAS  Google Scholar 

  16. T. B. Fitzpatrick, The validity and practicality of sun-reactive skin types I through VI, Arch. Dermatol., 1988, 124, 869–871.

    Article  CAS  Google Scholar 

  17. H. C. Wulf, A method and an apparatus for determining an individual’s ability to stand exposure to ultraviolet radiation, patent PCT/DK, 1993, WO 93/16635:1-44.

    Google Scholar 

  18. A. B. Hansen, N. Bech-Thomsen, H. C. Wulf, Erythema after irradiation with ultraviolet B from Philips TL12 and TL01 tubes, Photodermatol. Photoimmunol. Photomed., 1994, 10, 22–25.

    CAS  PubMed  Google Scholar 

  19. C. Tseng, B. Hoffman, I. Kurimoto, T. Shimizu, G. J. Schmieder, J. R. Taylor, J. W. Streilein, Analysis of effects of ultraviolet B radiation on induction of primary allergic reactions, J. Invest. Dermatol., 1992, 98, 871–875.

    Article  CAS  Google Scholar 

  20. V. E. Reeve, Ultraviolet radiation and the contact hypersensitivity reaction in mice, Methods, 2002, 28, 20–24.

    Article  CAS  Google Scholar 

  21. D. L. Damian, R. Sc. Barnetson, G. M. Halliday, Low-dose UVA and UVB have different time courses for suppression of contact hypersensitivity to a recall antigen in humans, J. Invest. Dermatol., 1999, 112, 939–944.

    Article  CAS  Google Scholar 

  22. A. A. Memon, P. S. Friedmann, Studies on the reproducibility of allergic contact dermatitis, Br. J. Dermatol., 1996, 134, 208–214.

    Article  CAS  Google Scholar 

  23. J. Lock-Anderson, N. D. Knudstorp, H. C. Wulf, Facultative skin pigmentation in Caucasians: an objective biological indicator of lifetime exposure to ultraviolet radiation?, Br. J. Dermatol., 1998, 138, 826–832.

    Article  Google Scholar 

  24. K. D. Cooper, L. Oberhelman, T. A. Hamilton, O. Baadsgaard, M. Terhune, G. LeVee, T. Anderson, H. Koren, UV exposure reduces immunization rates and promotes tolerance to epicutaneous antigens in humans: relationship to dose, CD1a-DR+ epidermal macrophage induction and Langerhans cells depletion, Proc. Natl. Acad. Sci. USA, 1992, 89, 8497–8501.

    Article  CAS  Google Scholar 

  25. L. Skov, H. Hansen, J. N. W. N. Barker, J. C. Simon, O. Baadsgaard, Contrasting effects of ultraviolet-A and ultraviolet-B exposure on induction of contact hypersensitivity in human skin, Clin. Exp. Immunol., 1997, 107, 585–588.

    Article  CAS  Google Scholar 

  26. H. Miyauchi, T. Horio, Ultraviolet B-induced local immunosuppression of contact hypersensitivity is modulated by ultraviolet irradiation and hapten application, J. Invest. Dermatol., 1995, 104, 364–369.

    Article  CAS  Google Scholar 

  27. H. Miyauchi-Hashimoto, T. Horio, Suppressive effect of ultraviolet B radiation on contact sensitization in mice. II. Systemic immunosuppression is modulated by ultraviolet irradiation and hapten application, Photodermatol. Photoimmunol. Photomed., 1996, 12, 137–144.

    Article  CAS  Google Scholar 

  28. P. S. Friedmann, The immunology of allergic contact dermatitis: the DNCB story, Adv. Dermatol., 1990, 5, 175–195.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, Medical University of Lodz, Krzemieniecka 5, Lodz, Poland

    Joanna Narbutt, Aleksandra Lesiak, Anna Wozniacka & Anna Sysa-Jedrzejowska

  2. Department of Dermatology, Basildon General Hospital, Essex, England, UK

    Malgorzata Skibinsk

  3. National Institute of Public Health and Environment, Bilthoven, The Netherlands

    Henk van Loveren

  4. Department of Pathomorphology, Medical University of Lodz, Lodz, Poland

    Iwona Lewy-Trenda & Aleksandra Omulecka

  5. Medical Microbiology, University of Edinburgh Medical School, Scotland, UK

    Mary Norval

Authors
  1. Joanna Narbutt
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  2. Aleksandra Lesiak
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  4. Anna Wozniacka
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  5. Henk van Loveren
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  7. Iwona Lewy-Trenda
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Correspondence to Joanna Narbutt.

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Narbutt, J., Lesiak, A., Skibinsk, M. et al. Suppression of contact hypersensitivity after repeated exposures of humans to low doses of solar simulated radiation. Photochem Photobiol Sci 4, 517–522 (2005). https://doi.org/10.1039/b503166d

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  • DOI: https://doi.org/10.1039/b503166d

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