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Detergent effect on Cytochrome bb559 electron paramagnetic resonance signals in the photosystem II reaction centre

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Abstract

The detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1-D2-Cyt b559 complex preparations. Various n-dodecyl-ß-D-maltoside concentrations from 0 to 0.2% (w/v) were used to stabilise the D1-D2-Cyt b559 complexes. Low spin heme EPR spectra were obtained but the gz feature positions changed depending on the detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (E′m = +123-150 mV) in all the D1-D2-Cyt b559 complex preparations indicating that detergent does not affect this property of the protein in those conditions. A similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein.

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References

  1. O. Nanba, K. Satoh, Isolation of a photosystem II reaction center consisting of D1 and D2 polypeptides and cytochrome b559, Proc. Natl. Acad. Sci. USA, 1987, 84, 109–112.

    Article  CAS  Google Scholar 

  2. X. S. Tang, K. Fushimi, K. Satoh, D1-D2 complex of the photosystem II reaction center from spinach. Isolation and partial characterization, FEBS Lett., 1990, 273, 257–260.

    Article  CAS  Google Scholar 

  3. W. R. Widger, W. A. Cramer, M. Hermodson, D. Meyer, M. Gullifor, Purification and partial amino acid sequence of the chloroplast cytochrome b-559, J. Biol. Chem., 1984, 259, 3870–3876.

    Article  CAS  Google Scholar 

  4. W. A. Cramer, J. Whitmarsh, Photosynthetic cytochromes, Annu. Rev. Plant Physiol., 1977, 28, 133–172.

    Article  CAS  Google Scholar 

  5. J. Whitmarsh, and H. B. Pakrasi, Form and function of cytochrome b559, in Oxygenic Photosysnthesis: The Light Reactions, ed. D. R. Ort and C. F. Yocum, Kluwer Academic Publishers, Dordrecht, The Netherlands. 1996, pp. 249–264.

    Google Scholar 

  6. D. H. Stewart, G. W. Brudvig, Cytochrome b559 of photosystem II, Biochim. Biophys. Acta, 1998, 1367, 63–87.

    Article  CAS  Google Scholar 

  7. A. Zouni, H.-T. Witt, J. Kern, P. Fromme, N. Krausse, W. Saenger, P. Orth, Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution, Nature, 2001, 409, 739–743.

    Article  CAS  Google Scholar 

  8. P. R. Rich, D. S. Bendall, The redox potentials of the b-type cytochromes of higher plant chloroplasts, Biochim. Biophys. Acta, 1980, 591, 153–161.

    Article  CAS  Google Scholar 

  9. J. M. Ortega, M. Hervás, M. Losada, Redox and acid-base characterization of cytochrome b559 in photosystem II particles, Eur. J. Biochem., 1988, 171, 449–455.

    Article  CAS  Google Scholar 

  10. O. Kaminskaya, J. Kurreck, K. D. Irrgang, G. Renger, V. A. Shuvalov, Redox and spectral properties of cytochrome b559 in different preparations of photosystem II, Biochemistry, 1999, 38, 16223–16235.

    Article  CAS  Google Scholar 

  11. N. Mizusawa, T. Yamashita, M. Miyao, Restoration of the high-potential form of cytochrome b559 of photosystem II occurs via a two-step mechanism under illumination in the presence of manganese ions, Biochim. Biophys. Acta, 1999, 1410, 273–286.

    Article  CAS  Google Scholar 

  12. M. Roncel, J. M. Ortega, M. Losada, Factors determining the special redox properties of photosynthetic cytochrome b559, Eur. J. Biochem., 2001, 268, 4961–4968.

    Article  CAS  Google Scholar 

  13. J. Bergstrom, T. Vanngard, EPR signals and orientation of cytochromes in the spinach chloroplast thylakoid membrane, Biochim. Biophys. Acta, 1982, 682, 452–456.

    Article  Google Scholar 

  14. L. K. Thompson, A.-F. Miller, C. A. Buser, J. C. de Paula, G. W. Brudvig, Characterization of the multiple forms of cytochrome b559 in photosystem II, Biochemistry, 1989, 28, 8048–8056.

    Article  CAS  Google Scholar 

  15. C. Berthomieu, A. Boussac, W. Mäntele, J. Breton, E. Nabedryk, Molecular changes following oxidoreduction of cytochrome b559 characterized by Fourier transform infrared difference spectroscopy and electron paramagnetic resonance: photooxidation in photosystem II and electrochemistry of isolated cytochrome b559 and iron protoporphyrin IX-bisimidazole model compounds, Biochemistry, 1992, 31, 11460–11471.

    Article  CAS  Google Scholar 

  16. G. T. Babcock, W. R. Widger, W. A. Cramer, W. A. Oertling, J. G. Metz, Axial ligands of chloroplast cytochrome b559: Identification and requirement for a heme-cross-linked polypeptide structure, Biochemistry, 1985, 24, 3638–3645.

    Article  CAS  Google Scholar 

  17. L. I. Krishtalik, G. S Cherepanov, W. A. Cramer, The redox properties of cytochromes b imposed by the membrane electrostatic environment, Biophys. J., 1983, 65, 184–195.

    Article  Google Scholar 

  18. D. A. Berthold, G. T. Babcock, C. F. Yocum, A highly resolved oxygen-evolving photosystem II preparation from spinach thylakoid membranes, FEBS Lett., 1981, 134, 231–234.

    Article  CAS  Google Scholar 

  19. G. Montoya, R. Cases, I. Yruela, R. Picorel, Spectroscopic characterization of two forms of the D1-D2-cytochrome b559 complex from sugar beet, Photochem. Photobiol., 1993, 58, 724–729.

    Article  CAS  Google Scholar 

  20. I. Yruela, P. J. M. van Kan, M. G. Müller, A. R. Holzwarth, Characterization of a D1-D2-cyt b559 complex containing 4 chlorophyll a/2 pheophytin a isolated with the use of MgSO4, FEBS Lett., 1994, 339, 25–30.

    Article  CAS  Google Scholar 

  21. I. Yruela, R. Tomás, M. Alfonso, R. Picorel, Effect of the pH on the absorption spectrum of the isolated D1-D2-cytochrome b559 complex of photosystem II, J. Photochem. Photobiol. B: Biol., 1999, 50, 129–136.

    Article  CAS  Google Scholar 

  22. I. Yruela, E. Torrado, M. Roncel, R. Picorel, Light-induced absorption spectra of the D1-D2-cytochrome b559 complex of photosystem II: effect of methyl viologen concentration, Photosynth. Res., 2001, 67, 199–206.

    Article  CAS  Google Scholar 

  23. C. Eijckelhoff, J. P. Dekker, A routine to determine the chlorophyll a, pheophytin a and ß-carotene contents of isolated Photosystem II reaction center complexes, Photosynth. Res., 1997, 52, 69–73.

    Article  CAS  Google Scholar 

  24. P. J. van Leeuwen, M. C. Nieveen, E. J. van de Meet, J. P. Dekker, H. J. van Gorkom, Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach, Photosynth. Res., 1991, 28, 149–153.

    Article  Google Scholar 

  25. D. Zheleva, B. Hankamer, J. Barber, Heterogeneity and pigment composition of isolated photosystem II reaction centers, Biochemistry, 1996, 35, 15074–15079.

    Article  CAS  Google Scholar 

  26. B. Hankamer, J. Nield, D. Zheleva, E. Boekema, S. Jansson, J. Barber, Isolation and biochemical characterisation of monomeric and dimeric photosystem II complexes from spinach and their relevance to the organisation of photosystem II in vivo, Eur. J. Biochem., 1997, 243, 422–429.

    Article  CAS  Google Scholar 

  27. C. P. S. Taylor, The EPR of low spin heme complexes, Biochim. Biophys. Acta, 1977, 491, 137–149.

    Article  CAS  Google Scholar 

  28. V. A. Shuvalov, R. Fiege, U. Schreiber, F. Lendzian, W. Lubitz, EPR study of cytochrome in the D1-D2-Cyt b559 complex, Biochim. Biophys. Acta, 1995, 1228, 175–180.

    Article  Google Scholar 

  29. B. Gall, H. Scheer, Stabilization of photosystem II reaction centers: influence of bile salt detergents and low pH, FEBS Lett., 1998, 431, 161–166.

    Article  CAS  Google Scholar 

  30. D. F. Ghanotakis, C. F. Yocum, Purification and properties of an oxygen evolving reaction center complex from photosystem II membranes, FEBS Lett., 1986, 197, 244–248.

    Article  CAS  Google Scholar 

  31. R. M. Garavito, S. Ferguson-Miller, Detergents as a tool in membrane biochemistry, J. Biol. Chem., 2001, 276, 32403–32406.

    Article  CAS  Google Scholar 

  32. M. le Maire, P. Champeil, J. V. Möller, Interaction of membrane proteins and lipids with solubilizing detergents, Biochim. Biophys. Acta, 2000, 1508, 86–111.

    Article  Google Scholar 

  33. M. Seibert, Biochemical, biophysical and structural characterization of the isolated Photosystem II reaction center complex, in The Photosynthetic Reaction Center, ed. J. Deisenhofer and J. R. Norris, Academic Press, San Diego, CA, 1993, vol. 1, pp. 319–356.

    Article  CAS  Google Scholar 

  34. G. Montoya, R. Cases, R. Rodríguez, M. Aured, R. Picorel, Detergent-induced reversible denaturation of the photosystem II reaction center: Implications for pigment-protein interactions, Biochemistry, 1994, 33, 11798–11804.

    Article  CAS  Google Scholar 

  35. F. A. Walker, B. H. Huynh, W. R. Scheid, S. R. Osvath, Models of the cytochromes b. Effect of axial ligand plane orientation on the EPR and Mössbauer spectra of low-spin ferrihemes, J. Am. Chem. Soc., 1986, 108, 5288–5297.

    Article  CAS  Google Scholar 

  36. F. A. Walker, D. Reis, V. L. Balke, Models of the cytochromes b. EPR studies of low-spin iron(III) tetraphenylporphyrins, J. Am. Chem. Soc., 1984, 106, 6888–6898.

    Article  CAS  Google Scholar 

  37. R. Quinn, M. Nappa, J. S. Valentine, New five- and six-coordinate imidazole and imidazolate complexes of ferric tetraphenylporphyrin, J. Am. Chem. Soc., 1982, 104, 2588–2595.

    Article  CAS  Google Scholar 

  38. J. C. Salerno, S. Yoshida, T. E. King, Effects of protein-protein and protein-lipid interactions on heme site conformation in the mitochondrial b cytochromes, J. Biol. Chem., 1986, 261, 5480–5486.

    Article  CAS  Google Scholar 

  39. B. Lanne, B. G. Malmström, T. Vänngard, The influence of pH on the EPR and redox properties of cytochrome c oxidase in detergent solution and in phospholipid vesicles, Biochim. Biophys. Acta, 1979, 545, 205–214.

    Article  CAS  Google Scholar 

  40. M. A. Noordermeer, G. A. Veldink, J. F. G. Vliegenthart, Spectroscopic studies on the active site of hydroperoxide lyase; the influence of detergents on its conformation, FEBS Lett., 2001, 489, 229–232.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo. 202, E-50080, Zaragoza, Spain

    I. Yruela, M. V. Ramiro & R. Picorel

  2. Instituto de Ciencia de Materiales de Aragón, Consejo Superior de Investigaciones Científicas-Universidad de Zaragoza, Plaza San Francisco s/n, E-50009, Zaragoza, Spain

    I. García-Rubio, J. I. Martínez & P. J. Alonso

  3. Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-Consejo Superior de Investigaciones Científicas, Americo Vespucio s/n, E-41092, Sevilla, Spain

    M. Roncel & J. M. Ortega

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  1. I. Yruela
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  2. I. García-Rubio
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Yruela, I., García-Rubio, I., Roncel, M. et al. Detergent effect on Cytochrome bb559 electron paramagnetic resonance signals in the photosystem II reaction centre. Photochem Photobiol Sci 2, 437–442 (2003). https://doi.org/10.1039/b300187c

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  • DOI: https://doi.org/10.1039/b300187c