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Figure 1: A schematic of the asymmetrical barcoding method. | Scientific Reports

Figure 1: A schematic of the asymmetrical barcoding method.

From: Asymmetrical barcode adapter-assisted recovery of duplicate reads and error correction strategy to detect rare mutations in circulating tumor DNA

Figure 1: A schematic of the asymmetrical barcoding method.

(a) Design of the asymmetrical random index sequencing adapter oligonucleotide. Self-annealing sites 1 and 2 are referred to as R1 and R2, respectively. (b) Sequencing library-preparation scheme using the asymmetrical random index sequencing adapter. The self-annealed barcodes in a were used in the adapter ligation step and subsequently amplified using the P7 flanking primer and the P5 index primer. (c) Comparison of the removal of duplicate candidates with a conventional duplication removal strategy. Molecules with the same barcodes and the same start/end positions were considered true duplicate pairs.

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