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5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation

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Abstract

5α-Androstane-3α,17β-diol (3α-diol) is considered to have no androgenic effects in androgen target organs unless it is oxidized to 5α-dihydrotestosterone (5α-DHT). We used microarray and bioinformatics to identify and compare 3α-diol and 5α-DHT responsive gene in human prostate LNCaP cells. Through a procedure called ‘hypervariable determination’, a similar set of 30 responsive genes involving signal transduction, transcription regulation, and cell proliferation were selected in 5α-DHT-, 3α-diol-, and epidermal growth factor (EGF)-treated samples. F-means cluster and networking procedures showed that the responsive pattern of these genes was more closely related between 3α-diol and EGF than between 5α-DHT and 3α-diol treatments. We conclude that 3α-diol is capable of stimulating prostate cell proliferation by eliciting EGF-like pathway in conjunction with androgen receptor pathway.

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Acknowledgements

This work was supported by the NIH Grant DK54925 to HKL.

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Authors and Affiliations

  1. Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R A Zimmerman, E H Nunlist, X Li, R Cowan, D J Culkin & H-K Lin

  2. Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

    I Dozmorov, Y Tang, M Centola & M B Frank

Authors
  1. R A Zimmerman
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  2. I Dozmorov
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  3. E H Nunlist
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  7. M Centola
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  10. H-K Lin
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Correspondence to H-K Lin.

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Zimmerman, R., Dozmorov, I., Nunlist, E. et al. 5α-Androstane-3α,17β-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation. Prostate Cancer Prostatic Dis 7, 364–374 (2004). https://doi.org/10.1038/sj.pcan.4500761

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